9,9-bis-(methoxymethyl)fluorene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L (nominal)
- Sampling method: Sample taken at t=0, 24h (before renewal), 24h (after renewal), 96h

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetonitrile
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 0.1 mL/L

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: Amazonas Commercial Aquarium, Shanghai
- Age at study initiation (mean and range, SD): 3 months
- Length at study initiation (mean and SD): 2.1 +/- 0.2 cm
- Weight at study initiation (mean and SD): 0.08 +/- 0.03 g

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): yes
- Feeding frequency during acclimation: fed everyday
- Health during acclimation (any mortality observed): not subnormal growth, no mortality

FEEDING DURING TEST
- Frequency: none - not fed during test

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg/L

Test temperature:

23°C +/- 1°C

pH:

7.78 - 8.3.1

Dissolved oxygen:

>70%

Nominal and measured concentrations:

mean measured concentrations

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 3L vessel
- Renewal rate of test solution (frequency/flow rate): once after 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light, 12 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Mortality and behaviour (after 3, 6, 24, 48, 72, 96 hours)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: limit of aqueous solubility

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: loading rate

Details on results:

- Behavioural abnormalities:all fish in test concentration were observed to be normal. One fish in each replicate experienced swimming disorders. No mortality was reported

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Mortality: yes
- LC50: 24h LC50 225 mg/L
- Other: expected 24h LC50 should between 200-400 mg/L

Sublethal observations / clinical signs:

The test substance remained stable throughtout the study confirmed by analytical determination.

Validity criteria fulfilled:

yes

Conclusions:

The 96h LC50 value was determined to be >100mg/L based on nominal concentrations and >1.6 mg/l based on mean measured concentration

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: Samples from the control, lowest, middle and highest concentrations were taken at t=24 (newly prepared media) and at t=48h from the same (spent media). Samples of ca. 100 mL were taken in brown glass bottles.
- Sample storage conditions before analysis: samples were analysed immediately after collection

Vehicle:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: No solvent was employed. Test medium with 100 mg/l of the
test substance was stirred for 20 hours and subsequently
left for 4 hours. The saturated solution was drawn off and
used as such or diluted with medium.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Length at study initiation (length definition, mean, range and SD): 2.25 +/- 0.19 cm
- Weight at study initiation (mean and range, SD): 0.096 +/- 0.027g

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

ca. 210 CaCO3 210 mg/L

Test temperature:

25 +/- 1C

pH:

8.0 - 8.3

Nominal and measured concentrations:

Measured

Details on test conditions:

TEST SYSTEM
- Test vessel: Beaker
- Material, size, headspace, fill volume: Glass, 2L, yes, 1.5L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): 24h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Ground water from Linschoten (The Netherlands)

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 8.12 mg/L

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 8.12 mg/L

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 8.12 mg/L

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 8.12 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: limit of solubility

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 8.12 mg/L

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

4.55 mg/L

Details on results:

% Concentration loss over test: 11 ... 29

Validity criteria fulfilled:

yes

Conclusions:

The 96h LC50 value was determined as >8.12 mg/L. This concentration was the limit of aqueous solubility. No mortality was observed in the test.

Description of key information

Two GLP-compliant, OECD guideline study conducted with Danio rerio indicate that no mortality or abnormal behaviour was seen up to the limit of aqueous solubility with 48h LC50 reported as >1.6 and >8.12 mg/l, respectively.

Additional information

It is not appropriate to include an LC50 value for the chemical safety assessment above as this is determined to be greater than the limit of aqueous solubility.