2-[(3-aminopropyl)methylamino]ethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-12-13 to 2007-04-09

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

results presented are based on nominal concentrations, no analytical verification of test concentrations performed

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Test Item Name: 2-[(3-Aminopropyl) methylamino]ethanol
- Label Identification: XTA-758
- Batch Number: PFW060026
- Expiration date of the lot/batch: not provided
- Purity test date: no data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage Conditions: Ambient
- Stability under test conditions: no data
- Solubility and stability of the test substance in the solvent/vehicle: no data

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: both definitive tests: concentrations of 100, 150, 225, 338 and 506 mg test substance/L in three replicates of each test concentration, with ten daphnids per replicate.
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no data

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water-flea
- Source: Stillmeadow, Inc. culture laboratory
- Age of parental stock: At the start of the test, the organisms used in the test were less than 24 hours old.
- Feeding during test: no

ACCLIMATION
- Acclimation period: no acclimatisation was necessary, since the breed of the daphnia was under identical conditions to those of the test.
- Health during acclimation (any mortality observed): The daphnia were healthy, no mortality was observed.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

40-180 mg/L CaCO3

Test temperature:

Definitive Test 1: 2007-02-13 to 2007-02-15: 20°C
Definitive Test 2: 2007-02-21 to 2007-02-23: 20°C

pH:

Definitive Test 1: 2007-02-13 to 2007-02-15
0h: 8.0-10.3
24h: 9.9-10.1
48h: 8.1.-9.8

Definitive Test 2: 2007-02-21 to 2007-02-23
0h: 8.0-10.2
24h: 9.9-10.1
48h: 7.9-9.7

Dissolved oxygen:

Definitive Test 1: 2007-02-13 to 2007-02-15
0h: 8.2-8.4 mg/L
24h: 7.6-7.8 mg/L
48h: 7.7-7.9 mg/L

Definitive Test 2: 2007-02-21 to 2007-02-23
0h: 8.7-8.9 mg/L
24h: 7.6-7.8 mg/L
48h: 7.1-7.2 mg/L

Salinity:

not relevant

Conductivity:

Definitive Test 1: 2007-02-13 to 2007-02-15
0h: 305-405 µmhos/cm
24h: 370-400 µmhos/cm
48h: 300-365 µmhos/cm

Definitive Test 2: 2007-02-21 to 2007-02-23
0h: 305-405 µmhos/cm
24h: 385-400 µmhos/cm
48h: 310-350 µmhos/cm

Nominal and measured concentrations:

Range-finder test
Nominal concentrations: 1.0, 10, 50, 100, 500 and 1000 mg/L

Test concentrations for both definitive tests:
Nominal concentrations: 100, 150, 225, 338 and 506 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers
- Material, size, headspace, fill volume: glass, 250 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no, static test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates):no

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Moderately hard synthetic freshwater with total hardness of 40-180 mg/L CaCO3 and a pH of 6.0-8.0

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:16-hour light to 8-hour dark cycle

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility of the daphnids and other non-lethal effects (e.g. abnormal behavior or appearance) after 24 and 48 hours of exposure.

RANGE-FINDING STUDY:
- Test concentrations: 1.0, 10, 50, 100, 500 and 1000 mg/L
- Results used to determine the conditions for the definitive study: yes.

Two definitive tests were conducted. The first test was conducted using nominal concentrations of 100, 150, 225, 338 and 506 mg/L. A mobility rate of 100% was observed in the control organisms. Mobility rates of 97% and 40% were observed in organisms treated with 100 mg/L and 150 mg/L resp. A 0% mobility rate was observed with 225, 338 and 506 mg/L.
The second test was conducted using the same nominal concentrations of 100, 150, 225, 338 and 506 mg/L. A mobility rate of 100% was observed in the control organisms. Mobility rates of 46% were observed in organisms treated with 150 mg/L. A 0% mobility rate was observed with 225, 338 and 506 mg/L.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

145.01 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% C.L.: 137.71-152.46 mg/L

Details on results:

- Behavioural abnormalities: no abnormalities in the control and the test concentration of 100 mg/L.
- Mortality of control: no
- Other adverse effects control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Reported statistics and error estimates:

The NOEC and EC50 were determined by a commercially available statistical program (ToxCalc Version 5.0). Several models are available for EC50 determination: Probit, Trimmed Spearmand-Karber and Linear Interpolation. The most appropriate model was selected for the determination in this case.

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test substance to Daphnia magna was determined in a 48 hour static test according to the approved protocol SOPs and OPPTS, series 850, section 1010. The test item had no acute toxic effects on Daphnia magna in a static 48-hour test up to the nominal concentration of 100 mg/L under the conditions of the test. Based on nominal concentrations, the 48-h EC50 was determined to be 145.01 mg/L with a 95% confidence interval ranging from 137.71 to 152.46 mg/L. The results of the test can be considered reliable without restriction. The results of the test can be considered reliable without restriction. It can be concluded that the test item is not hazardous to aquatic invertebrates.

Description of key information

The study by Rodrigue (2007), investigating the acute toxicity of the test substance in Daphnia magna according to SOPs and OPPTS, series 850, section 1010, was considered as the key study for endpoint coverage.

The 48-h EC50 was determined to be 145.01 mg/L (> 100 mg/L). Based on the results it can be considered that the test item is not harmful to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

145.01 mg/L