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EC number: 279-976-7 | CAS number: 82486-82-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- This study was conduced between 25 January 2018 and 01 February 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-(butylnitroamino)ethyl nitrate
- EC Number:
- 279-976-7
- EC Name:
- 2-(butylnitroamino)ethyl nitrate
- Cas Number:
- 82486-82-6
- Molecular formula:
- C6H13N3O5
- IUPAC Name:
- butyl(nitro)[2-(nitrooxy)ethyl]amine
Constituent 1
- Specific details on test material used for the study:
- Identification Butyl-NENA
NIVA substance number G128
CAS No. 82486-82-6
Batch No. 160002
REACH registration No. Not stated
EC No. Not stated
Purity 100 %. Added stabilizer n-Methyl p-Nitro Aniline, 0.5 %
Substance appearance Yellow liquid, oily
Date received 18.05.2017
Storage conditions Closed in bottle, dry at ambient temperature
Expiry data June 2021
Stability Stable under normal temperature conditions and recommended use. Explosive when mixed with oxidizing substances.
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Remarks:
- Elendt M7 medium
- Details on test solutions:
- Preparation of test solutions
A stock solution of 100 mg/L, the highest test concentration in the study, were prepared by adding 500 ml M7 to 50.06 mg butyl-NENA. This solution was also used to prepare the remaining test concentrations as indicated in the following table.
Concentration (mg/L) Volume stock solution (ml) Volume medium (ml) Final volume (ml)
Control 0 250 250
1 2.5 247.5 250
3.2 8 242 250
10 25 225 250
32 80 170 250
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Name Daphnia magna
Source In house cultures at NIVA (DHI – clone)
Culture details The Daphnia are cultured in Elendt M7 media under the same conditions as used in the test according to a standard operating procedure. Cultures are fed with Psedukirchineriella subcapitata.
Life-stage/Age Animals used for the test were young daphnia, less than 24 hours old
Justification Daphnia magna were used as the test organism as they are easily cultured within the laboratory and are recommended for use in freshwater ecotoxicity assessments [1].
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20 ± 2”C
- pH:
- 7 ± 1
- Details on test conditions:
- Exposure conditions
The exposure vessels were placed in a climate controlled room set at 20ºC with a photoperiod providing 16 hours light: 8 hours dark. Daphnids were not fed during the exposure period.
Addition of test animals
Offspring of D. magna (DHI-clone) were separated from in house cultures and were less than 24h old at the initiation of the test. The test animals were randomly selected and added to each test vessel using a wide bore pipette. Care was taken to minimise the amount of medium transferred (< 2 ml per replicate added). Twenty animals, 5 per replicate (4 replicates per concentration) were added per concentration.
Test procedure and Observations
All test vessels were rinsed with appropriate test solution prior to being filled for the test. After preparation of the test solutions and rinsing of test vessels, the test solutions were dispensed into the test vessels to give approximately 40 ml of solution per replicate. Test solutions were dispensed moving up the concentration gradient and starting with the control. The test vessels were identified by study number, concentration, and replicate number.
The study was initiated with the addition of the daphnids. At 24 hour intervals, observations were made for immobilisation and these data were recorded in the study book. Immobilisation is defined as the inability of the organism to swim within 15 seconds after gentle agitation of the test vessel, even if movement of antennae is observed. The daphnids were not fed during the study. At the start and end of the test, samples were removed for physicochemical analysis.
Physicochemical analysis
The pH was measured with an ORION STAR A211 pH meter in the control and highest test solution at the start of the test and after 48h.
Temperature was measured using min./max. thermometer with the sensor placed in water at the level of the test vessels. Temperature was recorded at the beginning and the end of the test.
Dissolved oxygen was measured with an InoLab OXI 7310 meter in the control and highest test solution at the start of the test and after 48h.
At the start of the test measurements were made on excess test solution, measurements at the end of the exposure were on solution from one replicate.
Data evaluation
For each test concentration and the control, the data from the replicates were pooled and percentage of immobilisation after 24 and 48h calculated (Table 2). The raw data was imported to ToxRatPro© (Version 3.2.1) where the OECD 202 template was used for the statistical analysis.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 96 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Validity criteria
The test met all the validity criteria (Table 1) required by OECD guidelines [1], and is considered valid. (Table 1).
Physicochemical parameters
The physicochemical parameters are showed in Table 2.
Data evaluation
No immobility was observed in the controls at 24h or 48h exposure. After 24h of exposure, immobility was observed for 1 of the 20 D. magna exposed to 32 mg/L, whereas 11 of the 21 D. magna exposed to 100 mg/L were immobile after 24h (Table 3). After 48h of exposure, 14 of 20 D. magna exposed to 32 mg/L were immobile, whereas 15 of 21 of the daphnia exposed to 100 mg/L were immobile after 48h. The results of the study are summarised in Table 4.
Any other information on results incl. tables
Table1. Summary of results for validity criteria
Parameter |
Criterion |
Observed |
Dissolved oxygen (at end of test) |
≥ 3 mg/L |
8.82-10.26 mg/L |
Percentage mortality in the control |
≤ 10% |
0 % |
Table2. Physico-chemical parameters
Concentration |
pH |
Dissolved oxygen (mg/L) |
Temperature (°C) |
|||
mg/L |
start |
end |
start |
end |
start |
end |
Control |
8.04 |
7.92 |
8.96 |
10.26 |
19.9-21.1 |
19.3-21.3 |
100 |
7.85 |
7.96 |
8.82 |
10.25 |
Table3. Survival data for D. magna at 24 and 48 hours after exposure toButyl-NENA
Test substance: Butyl-NENA |
|||||||||||||||||||||||||
Concentration (mg/L) |
24 hour (survival) |
48 hour (survival) |
Survived/added daphnia |
% mortality |
|||||||||||||||||||||
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
24 h |
48 h |
24 h |
48 h |
||||||||||||||
Control * |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20/20 |
20/20 |
0 |
0 |
|||||||||||||
1 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20/20 |
20/20 |
0 |
0 |
|||||||||||||
3.2 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
4 (1) |
20/20 |
19/20 |
0 |
5 |
|||||||||||||
10 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20/20 |
20/20 |
0 |
0 |
|||||||||||||
32 |
4 (1) |
5 |
5 |
5 |
0 (5) |
0 (5) |
4 (1) |
2 (3) |
19/20 |
6/20 |
5 |
70.0 |
|||||||||||||
100 |
2 (3) |
3 (2) |
2 (3) |
3 (3) |
1 (4) |
2 (3) |
1 (4) |
2 (4) |
10/21 |
6/21 |
52.4 |
71.4 |
*Shared control with study 17159/6 and 17159/7
Table 4. Summary of results
Test substance |
Butyl-NENA |
||||
Endpoint |
Concentration (mg/L) |
95 % Confidence intervals |
|||
24h NOEC |
32 |
NA |
|||
24h LOEC |
100 |
NA |
|||
24h EC50 |
96 |
70-174 |
|||
48h NOEC |
10 |
NA |
|||
48h LOEC |
32 |
NA |
|||
48h EC50 |
36 |
nd |
Where: NOEC = No observed effect concentration based on nominal loading.
LOEC = Lowest observed effect concentration based on nominal loading.
EC50represents the loading rate that results in 50 % immobilisation.
NA = Not applicable.
nd – not determined due to mathematical reasons
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 of the test substance for 24h and 48h exposure was 96 mg/L (24h EC50) and 36 mg/L (48h EC50). The NOEC and LOEC for immobility after 24h of exposure to butyl-NENA were 32 mg/L (NOEC) and 100 mg/L (LOEC). The NOEC and LOEC for immobility after 48h of exposure to butyl-NENA were 10 mg/L (NOEC) and 32 mg/L (LOEC).
- Executive summary:
The acute toxicity of Butyl-NENA to the freshwater crustaceanDaphnia magnawas investigated. The test was performed according to OECD 202 (2004)Daphnia sp.Acute immobilisation test [1]. The test animals were young daphnia, less than 24h old, from in house cultures (DHI clone) at NIVA.
A range of concentrations (1, 3.2, 10, 32, and 100 mg/L) were prepared in Elendt M7 medium. The M7 medium control was shared with study 17159/6 and 17159/7. Four replicate vessels, each containing fiveD. magnawere employed for the control and each treatment. Immobility was assessed at 24h and 48h.
The objective of the study was to determine the median effective concentration (EC50) of the test substance for 24h and 48h exposure, which was 96 mg/L (24h EC50) and 36 mg/L (48h EC50). The results are summarised as follows:
Test substance
Butyl-NENA
Endpoint
Concentration (mg/L)
95 % Confidence intervals
24h NOEC
32
NA
24h LOEC
100
NA
24h EC50
96
70-174
48h NOEC
10
NA
48h LOEC
32
NA
48h EC50
36
nd
Where: NOEC = No observed effect concentration based on nominal loading.
LOEC = Lowest observed effect concentration based on nominal loading.
EC50represents the loading rate that results in 50 % immobility.
NA = Not applicable.
nd – not determined due to mathematical reasons
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