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EC number: 258-548-3 | CAS number: 53423-65-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 July 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Reference Identification: Sodium 2,4,5-trichlorobenzenesulphonate CAS#: 53423-65-7; EC# 258-548-3 Common Name: STB-FR
Product Sample: Sodium Trichlorobenzene Sulfonate; (STB-FR) Lot: 1804-11 Approx. 300g
Date and Quantity Received: 05 Jun 18; 314.3 g (GW)
Physical Description: Solid, white powder
Storage Conditions: Room temperature
Purity: 100% active ingredients per Sponsor information - Analytical monitoring:
- not required
- Details on sampling:
- The control, test, and reference sample solutions were measured for its dissolved oxygen content independently in series such that each bottle is measured immediately after the expiration of the three hour contact time and after less than 10 minutes post contact time.
- Vehicle:
- no
- Details on test solutions:
- The test item was added to 234 mL of deionized water in a quantity appropriate for the desired concentration. Synthetic sewage (16 mL) and 250 mL of microbial inoculum (activated sludge) were added to each test bottle. The test item was prepared in the following concentrations: 10 mg/L, 100 mg/L, and 1000 mg/L
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The activated sludge sample was determined to have a salinity of 0.49 ppt, total residual chlorine of 0.78 mg/L, a temperature of 30C at collection, a pH of 7.3, dissolved oxygen of 4.76, and a conductivity of 1013 mhos prior to the wash/settling procedures. The sludge was washed three times and allowed to settle for approximately 20 hours. A sample of the sludge was weighed and dried to determine the level of suspended solids and the sludge was treated with 50 mL/L of synthetic sewage feed for storage.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Respiration rates were measured for up to 10 minutes immediately following the exposure period.
- Nominal and measured concentrations:
- Nominal: 10 mg/L, 100 mg/L, and 1000 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- Based on O2 consumption
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- Based on O2 consumption
- Results with reference substance (positive control):
- EC20 = 6.77 mg/L based on O2 consumption
EC50 = 9.43 mg/L based on O2 consumption - Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance did not inhibit the respiration rate of activated sludge at concentrations up to and including 1000 mg/L.
- Executive summary:
In a GLP OECD 209 guideline study designed to measure the respiration rate of an predominately domestic activated sludge fed with a standard amount of synthetic sewage feed, the EC20 and EC50 of the test item was determined to be >1000 mg/L and can therefore be considered to not inhibit activated sludge if discharged to sewage treatment plants.
Reference
Oxygen Dissolution and Consumption Rate of Blanks:
Sample |
Contact Time |
Dissolved oxygen (mg/L) |
Oxygen uptake rate (mg/L)/hr |
O2 Uptake rate (mg/g)/hr |
Blank 1 |
3 hours |
8.67 |
240.1 |
196.0 |
10 minutes |
2.00 |
|||
Blank 2 |
3 hours |
8.74 |
329.0 |
268.6 |
10 minutes |
2.00 |
|||
Average |
284.6 |
|||
Standard Deviation |
62.9 |
Oxygen Dissolution and Consumption Rate of Test Item:
Sample |
Contact Time |
Dissolved oxygen (mg/L) |
Oxygen uptake rate (mg/L)/hr |
% Inhibition * |
Test Item (10 mg/L) |
3 hours |
8.48 |
352.8 |
-24.0 |
10 minutes |
2.00 |
|||
Test Item (100 mg/L) |
3 hours |
8.95 |
2465 |
-766.3 |
10 minutes |
2.00 |
|||
Test Item (1000 mg/L) |
3 hours |
8.64 |
2033 |
-614.5 |
10 minutes |
2.00 |
|||
Test Item (1000 mg/L) |
3 hours |
8.43 |
2409 |
-746.6 |
10 minutes |
2.00 |
|||
Test Item (1000 mg/L) |
3 hours |
8.46 |
23365 |
-720.9 |
10 minutes |
2.11 |
|||
Mean of 1000 mg/L Replicates |
2259.3 |
-694.0 |
* Negative values for % inhibition are considered 0% inhibition.
Oxygen Dissolution and Consumption Rate of Reference Item:
Sample |
Contact Time |
Dissolved oxygen (mg/L) |
Oxygen uptake rate (mg/L)/hr |
% Inhibition |
Reference Item (5 mg/L) |
3 hours |
8.85 |
1727 |
-506.9 |
10 minutes |
2.00 |
|||
Reference Item (10 mg/L) |
3 hours |
8.67 |
438.0 |
-53.9 |
10 minutes |
2.01 |
|||
Reference Item (20 mg/L) |
3 hours |
9.37 |
281.8 |
1.0 |
10 minutes |
2.13 |
|||
Reference Item (40 mg/L) |
3 hours |
8.99 |
43.4 |
84.7 |
10 minutes |
4.44 |
Oxygen Dissolution and Consumption Rate of Abiotic Controls:
Sample |
Contact Time |
Dissolved oxygen (mg/L) |
Oxygen uptake rate (mg/L)/hr |
Abiotic A - Test Item (1000 mg/L) |
3 hours |
8.77 |
57.6 |
10 minutes |
8.26 |
||
Abiotic B - Test Item (1000 mg/L) |
3 hours |
8.81 |
43.9 |
10 minutes |
8.57 |
||
Abiotic C - Test Item (1000 mg/L) |
3 hours |
8.80 |
56.4 |
10 minutes |
8.35 |
||
Average |
50.8 |
Description of key information
In a GLP OECD 209 guideline study designed to measure the respiration rate of an predominately domestic activated sludge fed with a standard amount of synthetic sewage feed, the EC20 and EC50 of the test item was determined to be >1000 mg/L and can therefore be considered to not inhibit activated sludge if discharged to sewage treatment plants.
Key value for chemical safety assessment
Additional information
EC20 >1000 mg/L
EC50 >1000 mg/L
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