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EC number: 225-207-5 | CAS number: 4717-96-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-04-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of the test substance with a concentration of 5000 mg/L was prepared. - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Activated sludge was obtained from Kurume central sewage treatment center (Kurume-shi, Fukuoka, Japan) that consists of an aeration reactor of a sewage treatment plant receiving predominantly domestic sewage.
- Method of cultivation: The activated sludge (2.5 L) was fed with synthetic sewage (125 mL) and cultivated for 18.5h at 20 ± 2°C under aerobic condition in order to acclimate the sludge to the test temperature.
- Initial biomass concentration: 3020 mg/L (concentration of suspended solid) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- To reduce the loss of the test substance as it is volatile, exposure time was shorten to 30 minutes.
- Test temperature:
- 20 +- 2°C
- pH:
- 7.5 +- 0.5°C
- Nominal and measured concentrations:
- Nominal: 1000 mg/L
Measured: 540 mg/L (mean) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL glass vessel
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 mL
- Aeration: yes, airpump (controllable flow rate at 0.5 - 1 L/min)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
OTHER TEST CONDITIONS
- Adjustment of pH: No - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- >= 540 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid?
- Relevant effect levels: Yes, the EC50 of 3,5-dichlorophenol as reference substance was 13 mg/L and met the criterion values between 2 and 25 mg/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 was determined to be greater than 540 mg/L based on mean measured concentration.
- Executive summary:
An activated sludge respiration inhibition test according to OECD 209 and in compliance with GLP was conducted to determine the inhibition potential of Tetrahydr-4 -methyl-2H-pyran on microorganisms. The activated sludge obtained from a sewage treatment plant was exposed to the test substance in a nominal concentration of 1000 mg/L, equivalent to 540 mg/L mean measured concentration, for 30 minutes. The concentration was analytically verified by GC-FID. As no inhibition of the respiration of the microorganisms was observed, the EC50 was determined to be greater than 540 mg/L based on mean measured concentration.
Reference
Table 1: Oxygen consumption rate, percentage inhibition of respiration and measured concentration of the test substance after 30 minutes exposure.
Nominal loading concentration (mg/L) |
Measured concentration (mg/L) |
Oxygen consumption rate (mgO2/L_h) |
Percentage inhibition of respiration (%) |
Average percentage inhibition (%) |
1000 |
570 |
33.9 |
18 |
14 |
1000 |
540 |
34.2 |
17 |
|
1000 |
520 |
39 |
6 |
Description of key information
EC50 (30 min.) > 540 mg/L based on mean measured concentration
Key value for chemical safety assessment
- EC50 for microorganisms:
- 540 mg/L
Additional information
An activated sludge respiration inhibition test according to OECD 209 and in compliance with GLP was conducted to determine the inhibition potential of Tetrahydr-4 -methyl-2H-pyran on microorganisms. The activated sludge obtained from a sewage treatment plant was exposed to the test substance in a nominal concentration of 1000 mg/L, equivalent to 540 mg/L mean measured concentration, for 30 minutes. The concentration was analytically verified by GC-FID. As no inhibition of the respiration of the microorganisms was observed, the EC50 was determined to be greater than 540 mg/L based on mean measured concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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