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EC number: 225-713-6 | CAS number: 5025-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- For the calibration, a dilution series in acetonitrile/water 50/50 (% v/v) was prepared, using a stock solution. The method was validated for the parameters specificity, linearity, instru-ment repeatability, carryover, accuracy and precision. Stability of the test item was tested in a stock solution (1000 mg/L in demineralised water), standard solution samples with the concentration 50 mg/L in acetonitrile/water 50/50 (% v/v) and under test conditions for Daphnia immobilization test.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Genus Daphnia
Species magna
Strain STRAUS / BERLIN
Taxonomic position Arthropoda - Crustacea
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin - Test type:
- static
- Water media type:
- other: demineralised water
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Using a glass tube, the daphnia were caught and lifted from the beaker. They were put on a small sieve, and the medium surrounding the animals was sucked off using absorbent pa-per. Immediately after that, the animals were put into the respective test solution.
The test vessels were left to stand for 48 hours. After 24 and 48 hours, the immobilised daphnia were counted. Daphnia are considered immobilised when they do not perform any movements or are only able to move their antennae when the beaker is gently agitated. Daphnia which are trapped at the surface of the test solution are also considered immobi-lised.
The pH, the concentration of dissolved oxygen and the content of the test item in the test vessels were measured at the beginning and at the end of the test.
All values were documented in the raw data. - Test temperature:
- 19.5 – 20.3 °C
- Nominal and measured concentrations:
- The measured concentration at the beginning of the test was 100 % of the nominal concen-tration and after 48 hours the measured concentration was 106 % of the nominal concen-tration. Therefore, the determination of the biological results was based on the nominal concentration.
- Details on test conditions:
- Date of performance: 21. – 23. Jun. 2022
Treatment: 100 mg/L nominal concentration
The concentration to be tested was based on the result of a non-GLP pre-test.
Temperature: 19.5 – 20.3 °C
within one test, temperature did not vary more than 2 °C
Photo period: 16/8 hours, using LED’s
Duration: 48 hours
Observation times: 24 and 48 hours
Medium renewal: none
Replicates (Treatment): 4 vessels, each containing 20 ± 5 mL test solution and 5 daphnia
Replicates (Blank control): 4 vessels, each containing 20 ± 5 mL M4 medium and 5 daphnia - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol (C6H4Cl2O, CAS No. 591-35-5) was used as positive controls in a current reference study (202204R201). The 48 h-EC50 against 3,5-Dichlorphenol was determined as 2.04 mg/L.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Validity criteria fulfilled:
- yes
- Conclusions:
- The following result was determined for the test item 3-acetylthiazolidine-4-carboxylic acid (species: Daphnia magna):
48h-EC50 > 100 mg/L (nominal) - Executive summary:
One valid experiment was performed.
The study was performed as a limit test at the concentration 100 mg/L (nominal). For the test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
The tested concentration showed no toxicity. One of the animals was immobilised in the blank control which can be stated as not significant.
3,5-Dichlorphenol (C6H4Cl2O, CAS No. 591-35-5) was used as positive control in a current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV -determination. The concentration determined at the start of the test was 100 % of the nominal concentration. At the end of the test the determined concentration was 106 % of the nominal concentration. Therefore, the determination of the biological result was based on the nominal concentration (see Guidance Doc. No.23 (§176)).The following result was determined for the test item 3-acetylthiazolidine-4-carboxylic acid (species: Daphnia magna):
48h-EC50 > 100 mg/L (nominal)
Reference
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
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