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EC number: 614-114-9 | CAS number: 67746-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 September 2013 - 20 November 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- in accordance with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- (Prolongation up to 60 days, ammonium chloride omitted from medium to prevent oxygen consumption due to nitrification (no nitrogen limitation as shown by biodegradation of reference compound), inoculum activated sludge instead of effluent/surface water)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Linseed standoil
- IUPAC Name:
- Linseed standoil
- Reference substance name:
- 67746-08-1
- Cas Number:
- 67746-08-1
- IUPAC Name:
- 67746-08-1
- Details on test material:
- - Storage condition of test material: at ambient temperature in the dark
- Solubility in water: poor
- Calculated ThOD (Theoretical Oxygen Demand) of the test substance: 2.9 mg/mg
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Secondary activated sludge (12-09-13) from the STP Nieuwgraaf in Duiven, The Netherlands - treating predominatntly domestic wastewater.
- Storage conditions: activated sludge was preconditioned to reduce the endogenous respiration rates: 400 mg dry Weight (DW)/L of activated sludge was aerated for one week. Sludge was diluted in the biological oxygen demand (BOD) bottles (van Ginkel and Stroo, 1992). - Duration of test (contact time):
- 60 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Nutrients per liter of deionized water:
8.5 mg KH2PO4,
21.75 mg K2HPO4,
33.4 mg Na2HPO4•2H2O,
22.5 mg MgSO4•7H2O,
27.5 mg CaCl2,
0.25 mg FeCl3•6H2O.
Ammonium chloride was omitted to prevent nitrification.
- Solubilising agent: Dichloromethane (Sigma-Aldrich)
Sodium acetate was added to the bottles using an aqueous stock solution of 1.0 g/L. Linseed standoil was added to the bottles using a stock of 1.0 g/L in DCM.
For the Closed Bottle test, the test substance in DCM (0.6 mL) was directly added to the bottles. The bottles were placed on a roller bank in a ventilated hood for a few hours to allow the solvent to evaporate and to obtain an even distribution of the test substance on the walls of the bottles.
- Test temperature: 22°C -24°C
- pH: at day 0: 7.2
at day 28: 7.3 (both controls) and 7.2 (test)
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 0.30 L BOD (biological oxygen demand) bottles with glass stoppers
- Number of culture flasks/concentration: 10 bottles containing only inoculum; 6 bottles containing inoculum and sodium acetate; 10 bottles containing inoculum and test substance; and 10 bottles treated with DCM containing inoculum.
- Measuring equipment: electronical measurement of the oxygen concentration using an oxygen electrode (WTW TrioXmatic EO 200) and meter (WTW OXI 530) (Retsch, Ochten, The Netherlands); pH was measured using a Eutech Cyberscan pH11 pH meter (Eutech Instruments, Nijkerk, The Netherlands)
SAMPLING
- Sampling frequency: day 0, 7, 14, 21 and 28
- Sampling method: two duplicate bottles of all series were withdrawn for dissolved oxygen analyses on the respective days; One extenstion of the Closed bottle test by measuring the course of the oxygen decrease in the bottles of day 28 using a special funnel. An oxygen electrode was inserted in the BOD bottle to measure the oxygen concentration. Medium dissipated by the electrode flowed back into the BOD bottle after withdrawal of the oxgen electrode from the funnel (van ginkel and Stroo 1992). This method allowed measurement at day 42 and 60.
CONTROL AND BLANK SYSTEM
- Inoculum blank: inoculum alone and inoculum with DCM
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 33
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 41
- Sampling time:
- 60 d
- Remarks on result:
- other: prolonged Closed Bottle test
- Details on results:
- See table under 'Any other information'
The calculated theoretical oxygen demand (ThOD) of linseed standoil is 2.9 mg/mg and the ThOD of sodium acetate is 0.8 mg/mg.
The inhibition of the degradation of sodium acetate by the test substance in the Closed Bottle test was not determined due to irrelevent possible toxicity of linseed standoil to microorganisms degrading acetate. Inhibition of the endogenous respiration of the inoculum by the test substance at day 7 was not detected. Thus, no inhibition of the biodegradation expected.
BOD5 / COD results
- Results with reference substance:
- The biodegradation percentage of the reference compound, sodium acetate, at day 14 was 81.
Any other information on results incl. tables
Oxygen consumption (mg/L) and biodegradation (%) of linseed standoil (BOD/ThOD) and sodium acetate (BOD/ThOD) in the Closed Bottle test.
Time (days) |
Oxygen consumption (mg/L) |
Biodegradation (%) |
||
|
Test substance |
Acetate |
Test substance |
Acetate |
0 |
0.0 |
0.0 |
0 |
0 |
7 |
0.9 |
4.1 |
19 |
76 |
14 |
1.3 |
4.4 |
22 |
81 |
21 |
1.7 |
|
29 |
|
28 |
1.9 |
|
33 |
|
42 |
2.0 |
|
34 |
|
60 |
2.4 |
|
41 |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Difference of the replicate values at day 28 < 20%; Biodegradation percentage of the reference compound (sodium acetate) was 81 at day 14; Oxygen concentration > 0.5 mg/L in all bottles during the test period.
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Linseed standoil is biodegraded by 33% at day 28 and 41% at day 60 (prolonged Closed Bottle test). Linseed standoil is therefore not readily biodegradable, but can be classified as inherently biodegradable.
- Executive summary:
A ready biodegradability test in an aerobic aqueous medium was conducted to assess the biotic degradation of linseed standoil. The used Closed Bottle test was performed according to slightly modified OECD, EU and ISO Test Guidelines and in compliance with the OECD principles of Good Laboratory Practice.
Linseed standoil is considered non-inhibitory to the inoculum as it did not cause a reduction in the endogenous respiration. The Linseed stand was degraded by 33% on day 28 and 41% on day 60 in the Closed Bottle test. Thus the test substances revealed to not be readily biodegradable, but can be classified as inherently biodegradable.
The test fullfils the validity criteria by an endogenous respiration of 0.9 mg/L and by the total mineralization of the reference compound, sodium acetate. Sodium acetate was degraded by 81% of its theoretical oxygen demand after 14 days. Also, all bottles contained an oxygen concentration of >0.5 mg/L during the entire test period.
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