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Diss Factsheets
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EC number: 210-762-8 | CAS number: 622-97-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Federal Hazardous Substances Act , September 27, 1973
- GLP compliance:
- no
Test material
- Reference substance name:
- 4-methylstyrene
- EC Number:
- 210-762-8
- EC Name:
- 4-methylstyrene
- Cas Number:
- 622-97-9
- Molecular formula:
- C9H10
- IUPAC Name:
- 1-ethenyl-4-methylbenzene
- Test material form:
- liquid
- Details on test material:
- - Name as cited in study report: MCTR-243-77
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- New Zealand White rabbits weighing 2.05 to 2.15 kg.
Test system
- Type of coverage:
- open
- Preparation of test site:
- other: abraded and non-abraded site on each rabbit
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- Single dermal application of 0.5 mL to clipped and abraded rabbit skin. No occlusion. Not stated if wash off performed.
- Duration of treatment / exposure:
- Single application. 24 h exposure.
- Observation period:
- Observed at 24 and 72 h. Primary Index calculated.
- Number of animals:
- 3 male and 3 female
- Details on study design:
- The site of application was clipped and abraded on all rabbits. Observations were made at 24 and 72 h following application for signs of irritation and corrosivity. Irritation scores were recorded and the primary index was calculated. The test substance was applied to the backs and occlusive wrap was not used because the test substance reacted with the dental dam creating unwanted variable. The rabbits were restrained by being put in a Newman harness for the 24 h initial exposure.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- primary dermal irritation index (PDII)
- Basis:
- mean
- Time point:
- other: 24 and 72 h
- Score:
- 1.5
- Reversibility:
- other: reversibility not investigated
- Irritation parameter:
- erythema score
- Basis:
- animal: 1-6
- Time point:
- 24 h
- Score:
- 1.5
- Max. score:
- 2
- Reversibility:
- not fully reversible within: 72 h
- Remarks on result:
- other: for both intact and abraded skin
- Irritation parameter:
- edema score
- Basis:
- animal: 1-6
- Time point:
- 24 h
- Score:
- 0.3
- Max. score:
- 1
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- other: for both intacts and abraded skin
- Irritation parameter:
- erythema score
- Basis:
- animal: 1-6
- Time point:
- 48 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- edema score
- Basis:
- animal: 1-6
- Time point:
- 48 h
- Remarks on result:
- not measured/tested
- Irritation parameter:
- erythema score
- Basis:
- animal: 1-6
- Time point:
- 72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- not fully reversible within: 72 h
- Remarks on result:
- other: for noth intact and abraded skin
- Irritation parameter:
- edema score
- Basis:
- animal: 1-6
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 1
- Reversibility:
- fully reversible
- Remarks on result:
- other: for both intact and abraded skin
- Irritant / corrosive response data:
- Well defined erythema (score 2) in 3/6 rabbits with very slight edema (score 1) in other 3/6 rabbits was apparent at 24 h. At 72 h only very slight erythema (score 1) and edema (score 1) remained.
Mean value of > 1.5 - < 2.3 for erythema/eschar or for oedema from gradings in at least 2 of 3 tested animals from grades at 24, 48 and 72 h or, if reactions are delayed, from grades on 3 consecutive days after the onset of dermal reactions (when not included in the irritant category above).
In this study reactions were only measured at 24 and 72 h. However, at 24 hurs 3/6 animals had a score of 2 for erythema and 3/6 a score of 1. At 24 h 6/6 had a score of 1. Therefore the mean score for 3/6 rabbits is 1.5
Applicant's summary and conclusion
- Conclusions:
- Under the study conditions, the calculated primary index for the test substance was 1.5.
- Executive summary:
A study was conducted to determine the skin irritation/corrosion potential of the test substance in rabbits following a single dermal exposure according to Federal Hazardous Substances Act, September 27, 1973. Six New Zealand White rabbits (2.05 to 2.15 kg) were dosed dermally with a single application of 0.5 mL of the test substance. The site of application was clipped and abraded on all rabbits. Observations were made at 24 and 72 h following application for signs of irritation and corrosivity. Irritation scores were recorded and the primary index was calculated. The test substance was applied to the backs and an occlusive wrap was not used. Reactions were only measured at 24 and 72 h. At 24 h, 3/6 animals had a score of 2 for erythema and 3/6 a score of 1. At 24 h, 6/6 had a score of 1. Under the study conditions, the calculated primary index for the test substance was 1.5 (Adamik, 1997).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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