Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 258-380-0 | CAS number: 53126-67-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Please refer to the attached read-across justification in section 13. - Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: CL = 65 - 83 %
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-02-13 to 2012-04-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Empirical formula: C8H19O4P
- Molecular mass: 210.2 g/mol - Analytical monitoring:
- yes
- Remarks:
- HPLC-UV
- Vehicle:
- no
- Details on test solutions:
- - Pre-treatment of test item and preparation of test item concentrations:
A stock solution was prepared using 100.3 mg of the test item which were added to 1 litre of dilution water and treated for 1 h on a magnetic stirrer. The pH was measured to be 6.3.
To produce the different test item concentrations appropriate amounts of the stock solution were diluted with dilution water to a volume of 100 mL and 0.556 mL of the algal inoculum was added to each replicate resulting in a final cell density of 5000 cells/mL. For each test item concentration and the control 3 replicates were prepared. All flasks were sealed with cotton/cellulose stoppers. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- - Name: Desmodesmus subspicatus (formerly Scene-desmus subspicatus)
- Strain: No. 86.81 SAG
- Source: Strain of the test species obtained from 'The Collection of Algal Cultures' of the Institute of Plant Physiology at the University of Göttingen, Germany.
- Maintenance and Acclimatisation: Exponentially growing stock cultures were maintained in the test facility under constant temperature conditions (21 - 24 °C with a maximum fluctuation of ± 2 °C) at a light intensity in the range of 60 to 120 μE x m-2 x s-1 (measured in the range 400 to 700 nm using a spherical quantum flux meter). The growth medium (according to BRINGMANN & KÜHN (1977)) was renewed once a week. Cell density measurements were made using a microcell counter, Sysmex F300, Digitana.
- Preparation of pre-cultures: Pre-cultures were set up three days before the start of a test. They were grown under identical exposure conditions as the stock cultures, except from the use of a different growth medium. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21 - 24 °C
- pH:
- at the start of the test: 6.8 - 7.8
at the end of the test: 5.8 - 8.8 - Nominal and measured concentrations:
- Nominal concentrations: 3.1, 6.3, 12.5, 25, 50 and 100 mg/L
measured: For the nominal concentration of 3.1 mg/L the nominal concentration was 2.5 mg/L after 0 and 2.467 mg/L after 72 h. For the nominal concentration of 100 mg/L the nominal concentration was 103.039 mg/L after 0 and 91.452 mg/L after 72 h. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type: closed with cotton/cellulose stoppers
- Material, size, headspace, fill volume: glass, 300 mL, fill volume of 100 mL
- Initial cells density: 5000 cells/mL
- No. of organisms per vessel: 0.556 mL algal inoculum
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Light intensity and quality: 60 to 120 μE x m-2 x s-1 or an equivalent range of 4000 to 8000 lux
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Cell densities were measured in a microcell counter (Sysmex F300, Digitana) by taking small aliquots from each test flask, which were not replaced. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: CL = 65 - 83 %
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- The ErC50 and ErC10 of the test item on green algae were determined to be >100 mg/L and 76 mg/L, respectively. A NOEC of 50 mg/L was calculated.
- Executive summary:
A study was performed to assess the adverse effects of the test item on the growth rate of the freshwater algal species Desmodesmus subspicatus (former name: Scenedesmus subspicatus) over several generations. The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) and OECD Guideline for Testing of Chemicals No. 201 (2006). Exponentially growing algal cells were exposed for a period of 72 hours to a range of nominal concentrations - 3.1, 6.3, 12.5, 25, 50 and 100 mg/L of the test item dissolved in dilution water. To prepare the test media a magnetic stirrer was used. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate, relative to control cultures grown under identical conditions. Growth rates were also used to calculate a No Observed Effect Concentration and a Lowest Observed Effect Concentration (NOEC). During 72 hours exposure an ErC50 of >100 mg/L and ErC10 of 76 mg/L were measured and a NOEC of 50 mg/L was calculated.
Referenceopen allclose all
Analytical measurements
At the beginning and at the end of the test the test items concentration was determined analytically in the control and all test concentrations. The lowest test item concentration, 3.1 mg/L, slightly dropped below the 80 % recovery limit during the 72-hour exposure period. This is considered as not significant, because the highest determined test item concentration remained stable over the exposure time and, therefore, all results are expressed in terms of nominal concentrations. Effective concentrations ranged from 80.6 % to 103.0 % of nominal values at 0 hours, and from 79.6 % to 91.5 % of nominal values at 72 hours.
Table 1: Analysis
Test item concentration [mg/L] |
HPLC values [mg/L] |
|
|
0 h |
72 h |
Control |
n.d. |
< 0.0421 |
3.1 |
2.500 |
2.467 |
100 |
103.039 |
91.452 |
Table 2: pH values at the start and the end of the study
Test item concentration [mg/L] |
pH values |
|
|
0 h |
72 h |
Control |
7.8 |
8.8 |
3.1 |
7.8 |
8.5 |
6.3 |
7.8 |
8.4 |
12.5 |
7.7 |
8.3 |
25 |
7.6 |
8.2 |
50 |
7.3 |
8.0 |
100 |
6.8 |
5.8 |
Table 3: Growth rate dependent on concentration and time
Section-by-section growth rate in Desmodesmus subspicatus as dependent on concentration of the test item and time; Mean: arithmetic mean; Std.Dev.: standard deviation; CV: coefficient of variation (calculated from InputRawData)
Treatment [mg/L] |
Control |
3.1 |
6.3 |
12.5 |
25 |
50 |
100 |
Mean at 0 h |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Std. Dev. |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
CV |
|
|
|
|
|
|
|
Mean 0-24 h |
1.386 |
1.386 |
1.386 |
1.386 |
1.386 |
1.386 |
1.386 |
Std. Dev. |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
CV |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Mean 24-48 h |
1.297 |
1.386 |
1.426 |
1.386 |
1.504 |
1.381 |
1.099 |
Std. Dev. |
0.0771 |
0 |
0.0680 |
0 |
0 |
0.1257 |
0 |
CV |
5.943 |
0 |
4.770 |
0 |
0 |
9.105 |
0 |
Mean 48-72 h |
1.859 |
1.776 |
1.716 |
1.616 |
1.646 |
1.614 |
1.259 |
Std. Dev. |
0.1337 |
0.0653 |
0.1127 |
0.0621 |
0.0124 |
0.1394 |
0.1644 |
CV |
7.192 |
3.676 |
6.563 |
3.842 |
0.754 |
8.632 |
13.049 |
Table 4: Yield dependent on concentration and time
Yield of Desmodesmus subspicatus cells as dependent on concentration of the test item and time; Mean: arithmetic mean; Std.Dev.: standard deviation; CV: coefficient of variation (calculated from InputRawData)
Treatment [mg/L] |
Control |
3.1 |
6.3 |
12.5 |
25 |
50 |
100 |
Mean at 0 h |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Std. Dev. |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
CV |
|
|
|
|
|
|
|
Mean 24 h |
1.5 |
1.5 |
1.5 |
1.5 |
1.5 |
1.5 |
1.5 |
Std. Dev. |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
CV |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Mean 48 h |
6.833 |
7.5 |
7.5 |
7.5 |
8.5 |
6.5 |
5.5 |
Std. Dev. |
0.5774 |
0 |
0.5774 |
0 |
0 |
1 |
0 |
CV |
8.449 |
0 |
7.370 |
0 |
0 |
13.333 |
0 |
Mean 72 h |
46.5 |
46.833 |
45.833 |
39.833 |
46.167 |
39.5 |
20.833 |
Std. Dev. |
2.6458 |
3.0551 |
2.0817 |
2.5166 |
0.5774 |
1 |
3.5119 |
CV |
5.690 |
6.523 |
4.542 |
6.318 |
1.251 |
2.532 |
16.857 |
Description of key information
The ErC50 and ErC10 of the source item on green algae were determined to be >100 mg/L and 76 mg/L, respectively. A NOEC of 50 mg/L was calculated.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 76 mg/L
Additional information
No study on aquatic algae with the target substance is available. A read-across to Dibutyl hydrogen phosphate (CAS 107-66-4) was performed to address the endpoint of toxicity to aquatic algae and cyanobacteria.
A study was performed to assess the adverse effects of the test item on the growth rate of the freshwater algal species Desmodesmus subspicatus (former name: Scenedesmus subspicatus) over several generations. The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) and OECD Guideline for Testing of Chemicals No. 201 (2006). Exponentially growing algal cells were exposed for a period of 72 hours to a range of nominal concentrations - 3.1, 6.3, 12.5, 25, 50 and 100 mg/L of the test item dissolved in dilution water. To prepare the test media a magnetic stirrer was used. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate, relative to control cultures grown under identical conditions. Growth rates were also used to calculate a No Observed Effect Concentration and a Lowest Observed Effect Concentration (NOEC). During 72 hours exposure an ErC50 of >100 mg/L and ErC10 of 76 mg/L were measured and a NOEC of 50 mg/L was calculated.
In conclusion, the source substance showed no significant toxic effects on the tested animals. Therefore, it is expected that the target substance has no significant toxic effects on fish and the EC50 after 72 h exposure is >100 mg/L and the EC10 is 76 mg/L, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.