Registration Dossier
Registration Dossier
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EC number: 700-100-0 | CAS number: 39716-58-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pent-4-enoic acid
- EC Number:
- 209-732-7
- EC Name:
- Pent-4-enoic acid
- Cas Number:
- 591-80-0
- Molecular formula:
- C5H8O2
- IUPAC Name:
- pent-4-enoic acid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Source: Municipal sewage treatment plant, D-31137 Hildesheim
Pretreatment The activated sludge was washed twice with autoclaved tap water and maintained in an aerobic condition by aeration for 4 hours
and then homogenized with a mixer. Thereafter the sludge was filtered with folded filter and aerated with CO2-free air for 3 days. 25 mL/L were used to initiate inoculation.
Colony forming units of the inoculum: ca. 107 - 108 CFU/mL - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 35 mg/L
- Based on:
- other: analysed carbon content of 0.589 mg C/mg test item
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Duration: 28 d
Application: Once at test start
Test vessels: 2000 mL, Erlenmeyer flask
Volume of the test medium: 1000 mL
Water: Aqua demin.
Test medium: Mineral nutrient solution acc. to OECD 301 A / EC Directive 92/69 EC, C.4A
Temperature: 22 ± 2 °C
Photoperiod: Dark
Dispersion treatment: Continuously stirring
Samples were taken on days 0, 1, 3, 7, 14, 21 and 28.
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
- Test performance:
- toxicity control: 93 % elimination after 7 days, 98 % elimination after 28 days
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 3
- Sampling time:
- 1 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 92
- Sampling time:
- 3 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 95
- Sampling time:
- 7 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 96
- Sampling time:
- 14 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- day % degradation
1 57
3 92
7 96
14 97
21 100
28 99
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item must be regarded to be readily biodegradable in the 10-d-window and after 28 days.
The validity criteria of the test according to the guideline are fulfilled.
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