Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 294-304-2 | CAS number: 91697-43-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 01 Jul 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP - Guideline study, tested with the source substance ethyl linoleate (CAS No. 544-35-4). According to the ECHA guidance document “Practical guide 6: How to report read-across and categories (March 2010)”, the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Food and Consumer Product Safety Authority, Den Haag, The Netherlands
Test material
- Reference substance name:
- Ethyl linoleate
- EC Number:
- 208-868-4
- EC Name:
- Ethyl linoleate
- Cas Number:
- 544-35-4
- Molecular formula:
- C20H36O2
- IUPAC Name:
- ethyl octadeca-9,12-dienoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- A development and validation of an analytical method for the test substance in ISO medium was performed
- Concentrations: 0.5 and 12.0 mg/L
- Sampling method: additional test, separate samples to respiration inhibition test
- Sample storage conditions before analysis: fresh and stored at room temperature for at least 6 days
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Since the test substance was hardly soluble in water, test concentrations were prepared separately in Milli-RO water (tap water purified by reverse osmosis). Two 1-L test bottles were filled with 250 mL test substance mixtures with initial loading rates of 200 mg/L. These mixtures were stirred in closed dark brown glass bottles for 25 hours. Subsequently, 16 mL synthetic sewage feed, 200 mL sludge and Milli-RO water up to 500 mL were added resulting in a loading rate of 100 mg/L.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: Municipal sewage treatment plant: "Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving mainly domestic sewage
- Method of cultivation: Sludge was used one day after collection and kept aerated at test temperature until use
- Preparation of inoculum for exposure: Sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of sludge was weighed and dried overnight at ca. 105 °C to determine the amount of suspended solids
- Initial biomass concentration: 3.8 g suspended solids/L of sludge
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 18.3-18.9°C
- pH:
- 8.0 - 8.3
- Dissolved oxygen:
- constant aeration
- Nominal and measured concentrations:
- 0 and 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1L test bottles (glass)
- Volume: 500 mL
- constant stirring and aeration with clean oil-free air via pipette
- No. of vessels per concentration (replicates): 2
- No. of controls: 2
- No. of vessels per reference substance (replicates): three concentrations (5, 12 and 30 mg/L)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-RO water
- Following the overnight stirring period, synthetic sewage feed (16 mL) and the activated sludge (200 mL) were added to the test substance mixture. Milli-RO water was added to provide a final volume of 500 mL.
- Composition of synthetic sewage feed: 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2*2H2O, 0.2 g MgSO4*7H2O, 2.8 g K2HPO4; made up to 1 L dissolved in MilliQ water, pH was 7.5 ± 0.5
EFFECT PARAMETERS MEASURED: inhibition of the respiration rate
- after 3 hour contact time, a well mixed sample of the content was poured into a 300 mL oxygen bottle (glass) and the flask was sealed with an oxygen electrode connected to a recorder (flatbed recorder SE 102), forcing the air out of the vessel. Oxygen consumption was measured and recorded for approx. 10 minutes. During measurement, no aeration took place, but there was constant stirring via magnetic stirrer. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - The difference between controls was within 15% of each other
- Nominal analytical concentrations of 0.5 and 10.0 mg/L were analysed as 0.424, 0.393, 0.411, 0.362 and 0.406 mg/L and as 12.0, 11.6, 9.91, 10.2 and 10.3 mg/L, respectively - Results with reference substance (positive control):
- - The EC50 of 3,5-dichlorophenol was in the accepted range of 5-30 mg/L (9.3 mg/L)
- Regression line y = 51.34 x + 0.3, r2 = 0.9950
Any other information on results incl. tables
Oxygen concentration and oxygen consumption:
Flask | Conc. of test substance (mg/L) | Oxygen conc. at the start (mg O2/L) | Oxygen consumption (mg O2/L/h) | % Inhibition respiration rate |
control 1 | 0 | 7.6 | 41 | - |
control 2 | 0 | 8.0 | 42 | - |
T1 | 100 | 5.3 | 45 | -8* |
T2 | 100 | 6.9 | 42 | -*1 |
* A negative value indicates stimulation of the sludge. This value is considered as not significant
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.