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EC number: 207-623-9 | CAS number: 485-72-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 March - 19 November 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Lot No. of test material: 07-170-FIL-2/3
- Appearance: White powder
- Expiration date: May 2010
- Purity: >98.4%
- Storage condition of test material: Ambient - Analytical monitoring:
- yes
- Details on sampling:
- The concentration of test substance was determined in each test solution at 0 and 96 hours by standard analytical methods (not described in the report).
- Vehicle:
- yes
- Remarks:
- Dimethyl formamide (DMF)
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test material was determined to be insoluble in water. Therefore, a water-soluble fraction was prepared by adding the required nominal amount of test substance in moderately hard synthetic freshwater and mixing the solution for 20-24 hours. After this mixing time, the undissolved test substance was separated from the solution and only this water-soluble fraction was used for testing.
- Controls: Positive control (3 flasks treated with 10 mg/L zinc chloride) and solvent control (3 flasks containing 0.25 mL DMF in 500 mL of dilution water added to stock solution)
- Chemical name of vehicle: Dimethyl formamide (DMF)
- Concentration of vehicle in test medium: DMF was added as a solvent to the water-soluble fraction at 0.25 mL per 500 mL dilution in water. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Selenastrum capricornutum
- Source: StillMeadow, Inc. cultures
- Age of inoculum (at test initiation): 3 - 7 ± 1 days
- Method of cultivation: Three days prior to testing, 1000 mL of autoclaved algal stock culture medium (Woods Hole) was inoculated. This new mass culture was then placed in a ~25°C environmental chamber and maintained on continuous aeration. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- No data
- Test temperature:
- 24 ± 2°C (actual temperature 23°C)
- pH:
- INITIAL pH:
- Control 7.4
- 2.0 mg/L 7.4
TERMINAL pH:
- Control: 8.0 - 8.1
- Test vessels: 8.0 - 8.2
- Positive control: 7.3 - 7.4
- Solvent control: 8.3 - 8.5 - Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal: 0.25, 0.50, 0.75, 1.0 and 2.0 mg/L
Measured: See table 2 - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL sterile Erlenmeyer flasks with stoppers and aeration tubing; filled with 100 mL of the appropriate dilution
- Type: stoppered flasks
- Aeration: constant aeration
- Agitation: constant agitation at approximately 100 cycles/minute
- Initial cells density: 10000 cells/mL
- Control end cells density: 368 - 426 x 10^4 cells/mL after 96 hours
- No. of vessels per concentration (replicates): 3 flasks per test concentration
- No. of vessels per control (replicates): 3 flasks
- No. of vessels per vehicle control (replicates): 3 flasks
GROWTH MEDIUM
- Medium used: Algal stock culture medium (Woods Hole) with EDTA up to 0.3 mg/L
- Detailed composition: Not specified
TEST MEDIUM / WATER PARAMETERS
Not specified
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: No
- Photoperiod: continuous lighting
- Light intensity and quality: 400 ± 40 ft.c
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Observations of inhibition were made for each test container at 24, 48, 72 and 96 hours following dosing by measuring the mean cell density with a haemocytometer.
TEST CONCENTRATIONS
- Range finding study: There was one flask of test medium for each concentration. The algae were introduced to each concentration at an initial cell density of 10000 cells/mL and at 96 hours the terminal density was measured.
- Test concentrations: 1, 10, 100, 500 and 1000 mg/L
- Results used to determine the conditions for the definitive study: Not specified - Reference substance (positive control):
- yes
- Remarks:
- Zinc chloride (3 flasks treated with 10 mg/L)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- IC50
- Effect conc.:
- 0.24 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- - Control: The growth rate increased by a factor of at least 16 and the 96 hour terminal control increased to at least 3.5x10^6 cells/mL.
- Results with reference substance (positive control):
- - Results with reference substance valid?: Yes
- Validity criteria fulfilled:
- yes
- Remarks:
- The 72 hour interim control density and growth rate increased by a factor of at least 16 and the 96-hour terminal control increased to at least 3.5x10^6 cells/mL.
- Conclusions:
- The test concentration of Formononetin that resulted in 50% inhibition (IC50) was determined to be 0.24 mg/L with 95% confidence limits of 0.22 - 0.38 mg/L. The NOEC was determined to be <0.25 mg/L.
Reference
Table 1: Daily cell counts:
Test concentration (mg/L) |
Replicate |
Cell density x 104cells/mL |
|||
24 hours |
48 hours |
72 hours |
96 hours |
||
Control |
A |
10 |
24 |
76 |
368 |
B |
9 |
11 |
98 |
402 |
|
C |
5 |
22 |
111 |
426 |
|
0.25 |
A |
8 |
12 |
58 |
175 |
B |
5 |
18 |
84 |
191 |
|
C |
11 |
20 |
95 |
210 |
|
0.50 |
A |
6 |
8 |
43 |
134 |
B |
4 |
12 |
45 |
156 |
|
C |
5 |
8 |
58 |
117 |
|
0.75 |
A |
5 |
14 |
51 |
125 |
B |
6 |
12 |
59 |
152 |
|
C |
4 |
12 |
48 |
147 |
|
1.0 |
A |
4 |
16 |
44 |
119 |
B |
4 |
14 |
34 |
130 |
|
C |
3 |
7 |
40 |
123 |
|
2.0 |
A |
2 |
6 |
31 |
84 |
B |
4 |
7 |
35 |
91 |
|
C |
3 |
10 |
38 |
76 |
|
Positive control 10 mg/L ZnCl2 |
A |
0 |
0 |
1 |
0 |
B |
1 |
1 |
0 |
1 |
|
C |
0 |
2 |
1 |
1 |
|
Solvent control (DMF) |
A |
9 |
17 |
88 |
379 |
B |
7 |
28 |
95 |
362 |
|
C |
10 |
42 |
106 |
418 |
Table 2: Dose verification
|
Control |
mg/L |
Positive control |
Solvent control |
||||
Time point |
NA |
0.25 |
0.50 |
0.75 |
1.0 |
2.0 |
10 mg/L ZnCl2 |
DMF |
Day 0 |
0.53 |
0.58 |
0.57 |
0.61 |
0.59 |
0.68 |
0.51 |
0.52 |
96 hour |
0.65 |
0.56 |
0.69 |
0.54 |
0.58 |
0.61 |
0.63 |
0.59 |
Description of key information
A GLP study was performed in accordance with OECD Guideline 201, to determine the toxic effects of the test substance, Formononetin, on the growth of the freshwater algae Selenastrum capricornutum in a 96 hour test. The test concentration of Formononetin that resulted in 50% inhibition (IC50) was determined to be 0.24 mg/L with 95% confidence limits of 0.22 - 0.38 mg/L. The NOEC was determined to be <0.25 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.24 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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