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Diss Factsheets
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EC number: 947-854-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- No chemical analysis of test concentrations.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A pre-test was performed to examine the solubility of the test substance in the test medium. A solution was prepared at 100 mg/L. The test substance did not completely dissolve, even after half an hour of stirring. Instead, layer of foam appeared at the surface of the solution and undissolved test substance particles remained present. Treatment with ultra sonic waves increased the dissolving of the test substance substantially, but after overnight standing, the test substance had precipitated. Therefore, preparation of test solutions during the test included a stabilisation period of ca. 24 hours after preparation to obtain the maximum soluble concentration and at the same time to prevent testing with flocculated test substance. Preparation started with ultrasonic treatment and magnetic stirring as performed in the pre-test. The stabilised test solutions contained flocculated test substance and were filtered through a paper filter (Schleicher and Schuell 604) to remove the flocks of undissolved test substance (> ca. 5 µm). The resulting solutions (clear and colourless) were defined as the Water Accommodated Fractions (WAFs).
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age of parental stock (mean and range, SD): Maximum age of cultures was 4 weeks
- Feeding during test: none
- Breeding conditions: Each batch was started with newborn animals, i.e. less than three days old, by placing about 250 of them into 10 L of medium in an all-glass culture vessel. The temperature was 18-22 °C and daphnids were fed daily with a suspension of fresh water algae. The test medium was the same as for the exposure (M7 medium). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20.3 - 20.5 °C
- pH:
- 8.0 - 8.1
- Dissolved oxygen:
- 8.9 - 9.1 mg O2/L
- Nominal and measured concentrations:
- Nominal: control, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
Test vessel
- Material, size, headspace, fill volume: glass, 100 mL, headspace: 20 mL, fill volume: 80 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: same as test
- Intervals of water quality measurement: regularly
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED
- Mobility was recorded after 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: dilution range of 1-100% of a WAF prepared at 10 mg/L and to 100% WAF prepared at 100 mg/L.
- Results used to determine the conditions for the definitive study: A maximum of 10% immobilisation was recorded at the different dilutions tested. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Test solutions were clear and colorless after filtration. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Limit test: no
- Dose-response test: yes (control, 0.1, 0.18, 0.32, 056, 1.0, 1.8 mg/L)
- ECx: EC50 (48 h): 0.50 mg/L (95% CI: 0.45 - 0.61 mg/L)
Reference
Description of key information
EL50 (48 h) > 100 mg/L (nominal, OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
One experimental study is available investigating the short-term
toxicity of Fatty acids C16-18 (even numbered), mono and diesters with
sucrose (no CAS) to aquatic invertebrates. The study was performed
according to OECD 202 (GLP) with the water flea Daphnia magna.
A pre-test was performed to examine the solubility of the test substance
in the test medium. A solution was prepared at 100 mg/L. The test
substance did not completely dissolve, even after half an hour of
stirring. Instead, layer of foam appeared at the surface of the solution
and undissolved test substance particles remained present. Therefore,
preparation of test solutions during the test included a stabilisation
period of ca. 24 h after preparation to obtain the maximum soluble
concentration and at the same time to prevent testing with flocculated
test substance. Preparation started with ultrasonic treatment and
magnetic stirring as performed in the pre-test. The stabilised test
solutions contained flocculated test substance and were filtered through
a paper filter (Schleicher and Schuell 604) to remove the flocks of
undissolved test substance (> ca. 5 µm).
A Water Accommodated Fraction of nominal 100 mg/L was prepared and used
for testing. No chemical analysis was performed. However, the chemical
analysis in the acute fish study indicated that the substance is stable
in aqueous solutions. During the course of the test no immobilization of
D. magna was recorded and an EL50 (48 h) of > 100 mg/L was derived.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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