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EC number: 236-885-7 | CAS number: 13532-94-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Test phase 29-6-2020 to 3-7-2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Acclimatisation 7 days instead of 12 Test temp. 22.7 – 26.3oC. Fish stock tanks temp. 25.2 – 25.6oC
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: F1-9K024C
- Expiration date of the lot/batch: September 2020
- Purity test date: 99.5
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 4±2°C, protected from light
- Stability under storage conditions: stable
- Stability under test conditions: stable
- Solubility :395.7 mg/L - Analytical monitoring:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Individual aliquots of 2-Butoxyethyl methacrylate (EC 236-885-7) were weighed into separate glass aquaria containing 15L of media. At 48 hours the solution was stirred for 46 hours and 15 minutes. The first 100 – 200ml of aqueous phase was discarded and then the required volume (10L) of the remaining aqueous fraction was removed for use in the test. This was regarded as the test media. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra Fish
- Source:University College London
ACCLIMATION
- Acclimation period: 7 days
FEEDING DURING TEST
- Not fed during the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 22.7 – 23.9°C
- pH:
- 8.44 to 8.80
- Dissolved oxygen:
- >88.3% ASV
- Salinity:
- 0ppt
- Nominal and measured concentrations:
- 0 (control) 3mg/l 5mg/l 8mg/l 13mg/l 17.5mg/l reported as nominal as received and are expressed as loading rates, following preparation of water accommodated fractions (WAF’s).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 litre volume aquaria (H=20.5cm, W=20.5cm, L=30.5cm)
- Material, size, headspace, fill volume: glass
- Renewal rate of test solution (frequency/flow rate): test solutions replaced after 48 hours, the remaining live animals being transferred to freshly prepared test solutions.
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 1 replicate at 0 (control), 3, 5, 8, 13 17.5 mg/l.
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
The pH (to 0.01), dissolved oxygen (to 0.1% ASV), salinity (to 1ppt) and temperature (to 0.1°C) were measured in each test and control solution immediately prior to initiating the test. The test parameters were measured daily, before and after the change of test solution on day 2 and at the end of the 96-hour exposure period
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observations of the test media were made every 24 hours and prior to and after the renewal of solutions on day 2. Observations of the test organisms for mortality and visible sub-lethal effects were made after 1 hour, 24, 48, 72 and 96 hours as close to test initiation time as possible.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 2.0 and 17.4 mg/L
- Results used to determine the conditions for the definitive study: Initial results from this preliminary test indicated that the 96- hour EC50 was >17.4mg/l (by direct observation). - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: determined by direct observation as there was no mortality at 7.4mg/l. All calculations are based on nominal concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 4.592 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: (3.155 – 6.683mg/l LR)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 4.592 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LR (3.155 – 6.683mg/l LR)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 4.592 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LR (3.155 – 6.683mg/l LR)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 4.773 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LR (3.053 – 7.461mg/l LR)
- Sublethal observations / clinical signs:
See attached result table
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hour LC50 value of 2-Butoxyethyl methacrylate (EC 236-885-7) to Zebra fish was estimated to be 4.592mg/l LR (determined by Trimmed Spearman-Karber, trim = 42.9%). The 0 and 96hr NOEC and LOEC were determined by direct observation to be 3 and 5mg/l respectively. The lowest concentration giving 100% mortality of Zebra fish was >17.45mg/l LR. The full water quality measurements are given in the summary tables in Appendix 1 and were within acceptable limits. Specifically, the dissolved oxygen concentration remained greater than 60% of the air saturation value, which fulfils the validity criteria of the study.
No analytical confirmation of the test substance concentrations was performed, therefore, as 2- Butoxyethyl methacrylate (EC 236-885-7) was insoluble in water, concentrations are expressed as nominal LR’s. - Executive summary:
This study was undertaken to investigate the acute toxicity of 2-Butoxyethyl methacrylate (EC 236-885-7) to Zebra fish (Danio rerio). The objective of this study was to determine the 96-hour LC50 value, which is defined as the concentration that kills 50% of exposed fish after a 96-hour exposure period.
Test No. 203, Acute Toxicity Test to fish, OECD Guidelines for the Testing of Chemicals, Section 2 was used.
The 96 hour LC50 value of 2-Butoxyethyl methacrylate (EC 236-885-7) to Zebra fish was estimated to be 4.592mg/L LR (determined by Trimmed Spearman-Karber, trim = 42.9%). The 0 and 96hr NOEC and LOEC were determined by direct observation to be 3 and 5mg/L respectively. The lowest concentration giving 100% mortality of Zebra fish was >17.45mg/L LR. The full water quality measurements are given in the summary tables in Appendix 1 and were within acceptable limits. Specifically, the dissolved oxygen concentration remained greater than 60% of the air saturation value, which fulfils the validity criteria of the study.
No analytical confirmation of the test substance concentrations was performed, therefore, as 2- Butoxyethyl methacrylate (EC 236-885-7) was insoluble in water, concentrations are expressed as nominal LR’s.
Reference
Description of key information
The 96 hour LC50 value of 2-Butoxyethyl methacrylate (EC 236-885-7) to Zebra fish was estimated to be 4.592mg/l LR (determined by Trimmed Spearman-Karber, trim = 42.9%). The 0 and 96hr NOEC and LOEC were determined by direct observation to be 3 and 5mg/l respectively. The lowest concentration giving 100% mortality of Zebra fish was >17.45mg/l LR.
No analytical confirmation of the test substance concentrations
was performed, therefore, as 2- Butoxyethyl methacrylate (EC 236-885-7)
was insoluble in water, concentrations are expressed as nominal LR’s.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4.592 mg/L
Additional information
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