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EC number: 225-806-1 | CAS number: 5089-72-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-01-24 to 2002-02-01
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions. The study was conducted under GLP, however exposure concentrations were not measured.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 50 mg/L stock solution was prepared by placing 0.049 ml (density of 1.028 g/ml) of aminosilane in a 1000 mL volumetric flask and diluting to volume with sterile AAP medium containing 0.10 mL of dimethylformamide (DMF, CAS No. 68-12-2). The resulting stock solution was observed to be clear and colourless with no visible undissolved test substance. Nominal concentrations were prepared by dilution of the 50 mg/L stock solution. A solvent control was prepared with AAP medium containg 0.1 mL/L of DMF. A blank control comprising only of AAP medium was also prepared. - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: 1648
- Source (laboratory, culture collection): The alga was obtained from Carolina Biological Supply Co., Burlington, North Carolina, and was maintained in stock culture at Springborn Smithers.
- Culture conditions: The stock cultures were maintained within the following conditions: a shaking rate of 100 ± 10 rpm, a temperature of 24 ± 1 °C and continuous illumination at the surface of the medium with an intensity of approximately 300 to 500 footcandles (3200 to 5400 lux). Lighting was supplied by Duro-Test® Vita-Lite® fluorescent bulbs. Culture flasks were agitated continuously on an orbital shaker.
- Growth/test medium: The culture medium used was Algal Assay Procedure (AAP) medium prepared with sterile, deionized water.
- Culture vessesl: Stock cultures were grown in 250 ml glass flasks, each containing 100 ml of culture medium. The flasks were closed with stainless steel caps that permitted gas exchange. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- No data but conductivity measured at start and end of test ranged between 75 and 90 μmhos/cm
- Test temperature:
- 23 to 24 °C
- pH:
- 7.2-8.7 at start of test
7.8-8.2 at end of test - Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal test concentrations: 0 (Control), 0 (Solvent control), 1.6, 3.1, 6.3, 13, 25 and 50 mg/l
- Details on test conditions:
- TEST SYSTEM
- Growth/test medium: The culture medium used was Algal Assay Procedure (AAP) medium prepared with sterile, deionized water.
- Exposure vessel type: The test was conducted in sterile 250-ml Erlenmeyer flasks containing 100-ml of test solution. All test vessels were fitted with stainless steel caps which permit gas exchange.
- Culture medium: The culture mediumused was Algal assay Procedure (AAP) medium prepared with sterile deionised water. The TOC concentration of a sample collected in January 2002 was 0.47 mg/l.
- Light levels and quality during exposure: 320 - 420 footcandles (3400 - 4500 lux). The photosynthetically-active radiation (PAR) of the test area measured at test initiation ranged from 50 to 69 µE/m2/s.
- Test Design: Approximately 30 minutes after the test solutions were added to the test flasks (100 ml per flask), a 0.323-ml inoculum of Pseudokirchneriella subcapitata cells, at a density of approximately 310 x 10E4 cells/ml, was aseptically introduced into each flask. This inoculum provided the required initial (0 hour) cell density of approximately 1.0 x 104 cells/ml. Three replicate test vessels were established for each treatment level, the dilution water control and the solvent control.
- Determination of cell concentrations: At the start of the test and at each subsequent 24 observation period, cell counts were made on each three replicates of each treatment using a hemacytometer (Neubauer improved).
- Range finding test: The test concentrations were set on the basis of the results of a range finding test conducted at concentyrations of 0.00095, 0.0095, 0.095 and 0.95 mg/l and a second test conducted at concentrations of 60, 130, 240, 480 and 960 mg/l. No effects were observed in the first test and effects at all treatment levels were observed in the second test. - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- growth rate
- Remarks on result:
- other: 2.3-34 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- biomass
- Remarks on result:
- other: 1.8-17
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- biomass
- Remarks on result:
- other: determined empiracally as the highest concentration with <10% inhibition
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- other: cell density
- Remarks on result:
- other: 2.2-61 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- other: cell density
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Control cell density at end of test: 1580000 cells/ml (Control), 1360000 cells/ml (Solvent control), 1470000 cells/ml (Pooled control) - Reported statistics and error estimates:
- The EC50 values were determined by linear regression using the method of inverse regression.
The NOECs were determined by either Williams' test (if the data sets passed the test homogeneity and normality) or the Kruskal-Wallis'test if they did not. If the statistical analyses did not yield a reasonable NOEC it was selected empiracally as the highest treatment level that resulted in <10% inhibition.
All statistical determinations were made at 95% level of certainty. - Validity criteria fulfilled:
- yes
- Conclusions:
- A 72-hour EC50 value of 8.8 mg/l and NOEC of 3.1 mg/l have been determined for the effects of the test substance on growth rate of Pseudokirchnerella subcapitata. The results are expressed relative to nominal concentrations of the test substance. However the substance is subject to rapid hydrolysis and under the test conditions it is therefore likely that exposure will have been to its hydrolysis products (methanol and trisilanols).
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-11-29 to 1994-12-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Cited as Directive 92/69/EEC, C.3
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The Dissolved Organic Carbon (DOC) concentration of the stock solution used to prepare the test media and the test media was determined at athe start and end of the test
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was added to the dilution medium at 1 g/l and stirred for 18 hours. After that the solution was filtered and the DOC determined. The stock solution was used to prepare the treatments by dilution. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: 86.81.SAG
- Source (laboratory, culture collection): Institute for Water, Ground and Air Hygiene, Berlin (Germany)
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: A pre-culture is produced from an original culture by super-inoculation three days before the test begins. From this culture the test cultures are inoculated at a density of about 20000 cells/ml. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- No data
- Test temperature:
- 24 +/-1ºC
- pH:
- 6.9-8.4 at start of test
8.6-9.5 at end of test - Dissolved oxygen:
- Not reported
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal test concentrations (based on measured DOC concentration in stock solution converted to the equivalent test substance concentration): 0 (Control), 4.4, 9.1, 20, 44, 91, 200 and 436 mg/l.
The measured concentrations at the start and end of the test were with 20% of the nominal values and therefore the test results are interpreted with reference to nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open Erlenmeyer flasks
- Material: glass
- Aeration: none
- Initial cells density: 20000 cells/ml
- Control end cells density: 660000 cells/ml
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no
- Intervals of water quality measurement: start and end of test
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 8000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Algal cell counts were made photometrically at 0, 24, 48 and 72 hrs.
- Determination of cell concentrations: spectrophotometer (absorption at 685 nm)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.1-2.2 - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- other: biomass and growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 126 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 59 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 352 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Reported statistics and error estimates:
- EC50 values were determined by Probit Analysis
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 72-hour EC50 value of 352 mg/l and NOEC of 20 mg/l have been determined for the effects of the test substance on growth rate of Scenedesmus subspicatus (new name: Desmodesmus subspicatus). The results are expressed relative to nominal concentrations of the test substance. However the substance is subject to rapid hydrolysis and under the test conditions it is therefore likely that exposure will have been to its hydrolysis products (methanol and trisilanols).
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the attached justification for grouping of substances in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- growth rate
- Remarks on result:
- other: 2.3-34 mg/L
- Remarks:
- Springborn Smithers 2002
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- Springborn Smithers 2002
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 352 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- Hüls 1994
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 59 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- Hüls 1994
Referenceopen allclose all
Table 1. Test results
Nominal concentration (mg/l) |
Mean cell concentration after 24 hours (cells/ml) |
Mean cell concentration after 48 hours (cells/ml) |
Mean cell concentration after 72 hours (cells/ml) |
Mean cell concentration after 96 hours (cells/ml) |
Percentage reduction in cell concentration at end of the test* |
Percentage reduction in biomass at end of the test* |
Percentage reduction in 0 -72 -hour growth rate* |
0 (Control) |
24200 |
75000 |
435000 |
1580000 |
- |
- |
- |
0 (Solvent control) |
21700 |
75000 |
408000 |
1360000 |
- |
- |
- |
0 (Pooled control) |
20000 |
80000 |
420000 |
1470000 |
- |
- |
- |
1.6 |
17500 |
75800 |
390000 |
1420000 |
3 |
7.0 |
2.0 |
3.1 |
10800 |
52500 |
413000 |
1590000 |
-8 |
14 |
1.0 |
6.3 |
7500 |
35800 |
256000 |
1220000 |
17 |
48 |
15** |
13 |
800 |
10000 |
87000 |
860000 |
41** |
89 |
44** |
25 |
800 |
800 |
3000 |
10000 |
99** |
108** |
128** |
50 |
800 |
5000 |
3000 |
0 |
100** |
106 |
128** |
*A negative sign (-) indicates that biomass or growth rate was higher than in the Control.
** Significantly different compared with pooled control
Table 1. Test results
Nominal concentration (mg/l) | Nominal cell concentration at start of test (cells/ml) | Mean cell concentration after 24 hours (cells/ml) | Mean cell concentration after 48 hours (cells/ml) | Mean cell concentration after 72 hours (cells/ml) | Percentage reduction in biomass at end of the test* | Percentage reduction in 0 -72 -hour growth rate* |
0 (Control) | 20000 | 80000 | 230000 | 660000 | - | - |
4.4 | 20000 | 60000 | 170000 | 740000 | 6.8 | -3.3 |
9.1 | 20000 | 70000 | 210000 | 850000 | -11.0 | -7.2 |
20 | 20000 | 60000 | 200000 | 900000 | -3.4 | -5.5 |
44 | 20000 | 70000 | 170000 | 540000 | 22.0 | 5.7 |
91 | 20000 | 70000 | 130000 | 310000 | 48.3 | 21.6 |
200 | 20000 | 60000 | 100000 | 180000 | 66.1 | 37.2 |
436 | 20000 | 60000 | 60000 | 110000 | 78.8 | 51.3 |
*A negative sign (-) indicates that biomass
or growth rate was higher than in the Control.
Description of key information
ErC50 (72 h)= 8.8 mg/l and NOErC (72 h)= 3.1 mg/l based on read across from CAS 1760 -24-3 (OECD 201)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 8.8 mg/L
- EC10 or NOEC for freshwater algae:
- 3.1 mg/L
Additional information
Measured data are not available for N-[3-(triethoxysilyl) propyl]ethylenediamine (CAS 5089-72-5), therefore read across from the comparable substance, N-(3 (trimethoxysilyl) propyl) ethylenediamine (CAS 1760-24-3) was deemed acceptable for assessment.
A 72 h EC50 value of 8.8 mg/l and NOEC of 3.1 mg/l have been determined for the effects of the test substance on growth rate of Pseudokirchneriella subcapitata (2002). The results are expressed relative to nominal concentrations of the test substance. However the substance is subject to rapid hydrolysis under the tested conditions, it is therefore likely that exposure will have been to its hydrolysis products (methanol and N-(3-(trihydroxysilyl) propyl) ethylenediamine). This result has been selected as the lowest EC50 value for effects on growth rate from four reliable studies.
A further study on the analogue substance N-(3 (trimethoxysilyl) propyl) ethylenediamine (CAS 1760-24-3) is available (1994). Desmodesmus subspicatus was exposed to nominal concentrations of 0 (Control), 4.4, 9.1, 20, 44, 91, 200 and 436 mg/l. The study resulted in a 72 -h EC50 value of 352 mg/l and a corresponding NOEC of 59 mg/l.
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