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EC number: 611-575-8 | CAS number: 577953-88-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 25th to January 28th 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Done under GLP and OECD methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Method: other: "Algal inhibition test" (C.3) Directive 67/548/EWG (Draft 1992), comparable to OECD TG 201
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dicyclohexylamine
- EC Number:
- 202-980-7
- EC Name:
- Dicyclohexylamine
- Cas Number:
- 101-83-7
- IUPAC Name:
- N-cyclohexylcyclohexanamine
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Purity: 99.71%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- MONITORING DATA: - cell density measurements of growth (daily) - analysis: twice (at test start, after 72 h)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- test concentration: 8 (0.008, 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1.0 mg/l) plus 1 control - application of test substance: mixed using an ultraturrax 8000 rpm for 1 m
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM: - stock culture: non-axenic strain obtained from the Institute of Plant Physiology at the University of Göttingen (Germany); exponentially-growing stock cultures maintained under constant temperature conditions (23 +/- 2 °C), light intensity (120 µE / m² x s); nutrient medium renewed once a week; cell density measured using a microcell counter - preculture: set up three days before test started; grown under identical exposure conditions except from the use of a different nutrient medium
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- pH:
- - pH: 8.2 (controls at test start), 10.4 (controls after 72); 8.3 (test substance incubations at test start), 10.3-10.4 (test substance incubations after 72 h)
- Details on test conditions:
- Test vessel: 300 ml Erlenmeyer flasks with ground glass stoppers (closed system) - culturing apparatus: temperature 21 to 25 °C, continuous uniform illumination (spectral range 400 to 700 nm) - light intensity: 120 µE / m² x s - test cultures: final cell density 10 E+4 cells/ml - test concentration: 8 (0.008, 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1.0 mg/l) plus 1 control - application of test substance: mixed using an ultraturrax 8000 rpm for 1 min
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.02 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- not specified
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- not specified
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- not specified
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.016 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- not specified
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.031 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- not specified
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 0.063 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- not specified
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 0-72 hr ErC50 of test substance biomass was calculated to be 0.38 mg/l. The 0-72hr ErC50 of test substance growth rate was calculated to be > 1 mg/l and NOEC 0.016 mg/l.
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