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EC number: 200-644-4 | CAS number: 67-16-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 9 September 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide
- EC Number:
- 200-644-4
- EC Name:
- N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide
- Cas Number:
- 67-16-3
- Molecular formula:
- C24H34N8O4S2
- IUPAC Name:
- N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- Not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- To prepare the stock solution, the test item was dissolved or dispersed directly in M4 reconstituted water. The pH of this solution (M4) is 8.0 ± 0.5 and the sum of Ca and Mg ions in the solution is 2.5 mmol/L with a molar ratio of Ca to Mg ions of approximately 4:1, and of Na to K of 10:1. The obtained preparation was agitated mechanically for up to 24 hours in an attempt to completely dissolve or disperse the test item.
Test solutions was prepared by further dilution of the stock solution with M4 or directly from the test item. A solution containing a maximum of 100 mg/L of the test item plus two other range-finding concentrations was prepared. Test vessels was filled directly from the test solution containers immediately after preparation. The pH range considered as appropriate for a normal life of test organisms is circa 6 to 9.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna STRAUS, clone 5.
Reason for this choice: most sensitive clone of the species recommended in OECD and EEC guidelines.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Animals were inspected for immobilization at TO, T24 and T48 hours.
Test conditions
- Hardness:
- 272 mg/L at T0 and 289 mg/L at T48 on the control flask
- Test temperature:
- 19,7°C mini
20.2°C maxi - pH:
- pH = between 8.13 & 8.20 at T0
ph = between 8.35 & 8.37 at T48 - Dissolved oxygen:
- between 8.6 & 8.7 mg/L at T0
between 8.7 & 8.8 mg/L at T48 - Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal concentration of 1, 10 and 100 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobilisation was observed at tested concentrations.
Any other information on results incl. tables
Table I :physicochemical parameters
Control |
||||
Time |
Hardness (mg/L) |
Oxygen levels % |
Oxygen levels (mg/l) pH |
|
T 0 hours T 48 hours |
272 289 |
98 98 |
8,7 8,8 |
8,20 8,37 |
Highest tested concentration |
||||
Time Oxygen level % |
Oxygen level (mg/L) |
pH |
Nominal charge rate (mg (L) |
|
T 0 hours T 48 hours 97 |
8,6 8,7 |
8,13 8,35 |
100 100 |
Temperature measured in water between T2 hours and TO time: 19.8°C.
Temperature recorded in a beaker of water dilution placed in the conditions of the study: minimum 19.7°C / maximum 20.2°C.
Table 2:Summary table of mortality
Immobilization (n on 5 daphnia) at each nominal rate (mg/L)
|
0 |
1 |
10 |
100 |
|
Replicat 1 2 3 4 |
Replicat 1 2 3 4 |
Replicat 1 2 3 4 |
Replicat 1 2 3 4 |
T 0 hour |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
T 24 hours |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
T 48 hours |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
Applicant's summary and conclusion
- Conclusions:
- The highest rate of charge without immobilisation at T48h was > 100 mg/L.
The lowest charge rate with 100% of immobilisation at T48h was > 100 mg / L. - Executive summary:
The highest rate of charge without immobilisation at T48h was > 100 mg/L.
The lowest charge rate with 100% of immobilisation at T48h was > 100 mg / L.
EC50s at 24 h and 48 h were > 100 mg/L (charge rate).
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