Octadecane-1-thiol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in mammalian cells

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

comparable to guideline study GLP, Comparable to OECD 476 and OPPTS 870.5300 Reliability 1

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)

Deviations:

yes

Remarks:

yes Information on number of cells used and other important parts of the experimental design are not provided.

Principles of method if other than guideline:

Similar to OECD 476 and OPPTS 870.5300

GLP compliance:

yes

Type of assay:

in vitro mammalian cell gene mutation tests using the thymidine kinase gene

Test material

Test material information

Constituent 1

Reference substance name:

Dodecane-1-thiol (CAS # 112-55-0)

IUPAC Name:

Dodecane-1-thiol (CAS # 112-55-0)

Method

Target gene:

TK+

Species / strain

Species / strain / cell type:

other: L5178 TK+/- lymphoma cell

Metabolic activation:

with and without

Test concentrations with justification for top dose:

30, 20.1, 13.5, 9.0, 6.0, 4.0, 2.7, and 1.8 µg/mL

Vehicle / solvent:

Solvent control used

Controls

Untreated negative controls:

yes

Positive controls:

yes

Remarks:

ethylmethane sulfonate (620 ug/ml) without metabolic activation and 3-methylholanthrene (3 ug/ml) with metabolic activation

Positive control substance:

3-methylcholanthrene

ethylmethanesulphonate

Results and discussion

Test results

Key result

Species / strain:

other: mouse lymphoma L5178Y cells mammalian cell line

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: other: Acceptable growth inhibition exhibited at 30 ug/ml.

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

not measured/tested

Any other information on results incl. tables

Cells  exposed to eight  graded  doses of test substance with and  without  metabolic  activation  did  not  exhibit  a dose-dependednt increase in mutation frequency at the TK +/- locus. A test was considered positive if dose-related response at two or more test concentrations is at least two or three-fold higher than mutation frequency of solvent control.

Applicant's summary and conclusion

Conclusions:

Interpretation of results: Negative

Executive summary:

In a mammalian cell gene mutation assay focused on the T/K locus, L5178Y Mouse Lymphoma cells cultured in vitro were exposed to dodecane-1-thiol at concentrations at 1.8, 2.7, 4.0, 6.0, 9.0, 13.5, 20.1, and 30.0 µg/mL in the presence and absence of mammalian metabolic activation.    
 
Concurrent  positive  controls  were  ethylmethane  sulfonate  (620  ug/ml)  without  metabolic activation  and  3-methylcholanthrene  (3  ug/ml)  with  metabolic  activation.  A  negative and solvent control were also run concurrently. Under experimental conditions, dodecane-1thiol did not
exhibit a positive response and is considered to be not mutagenic in this test system. This study received a Klimisch score of 1 and is classified as reliable without restriction because the study adhered closely to OECD guidelines.