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EC number: 238-692-3 | CAS number: 14643-87-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 22, 2018 to January 25, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
- Version / remarks:
- Adopted Jul. 29, 2016
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.40 (In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test (TER))
- Version / remarks:
- Dated May 30, 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Zinc acrylate
- EC Number:
- 238-692-3
- EC Name:
- Zinc acrylate
- Cas Number:
- 14643-87-9
- Molecular formula:
- C3H4O2.1/2Zn
- IUPAC Name:
- zinc acrylate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Test Substance Name: Zinc acrylate
Batch no: SC1703131C
Test Facility Identification Number: 17102308G
Appearance: White powder
Composition: 99.5% Zinc acrylate
Purity: 99.5% Zinc acrylate
Homogeneity: Homogeneous
Expiry date: Sep. 09, 2018
Storage: Room emperature: (20 ± 5°C), keep away from humidity.
Constituent 1
In vitro test system
- Test system:
- other: EpiDermTM Kit (EPI-212-SCT; Batch# 25875)
- Source species:
- other: Human-derived
- Cell type:
- other: Human-derived epidermal keratinocytes which have been cultured to form a multi-layered, highly differentiated model of the human epidermis.
- Cell source:
- other: Supplier: MatTek In Vitro Life Science Laboratories, Bratislava.
- Source strain:
- not specified
- Justification for test system used:
- The test system consists of organized basal, spinous and granular layers, and a multi-layered stratum corneum containing intercellular lamellar lipid layers arranged in patterns analogous to those found in vivo.
- Vehicle:
- water
- Remarks:
- Demineralised water, prepared by LAUS GmbH, from an ion-exchanger, batch no: 20170815.
- Details on test system:
- - Environmental Condition during incubation / exposure:
37 ± 1°C and 5.0 ± 0.5% CO2
- Amount of test substance applied: 26.0 and 26.1 mg (3 minutes exposure); 26.6 mg (1 h exposure) - Control samples:
- yes, concurrent vehicle
- yes, concurrent positive control
- Amount/concentration applied:
- 3 minutes exposure: 26.0 mg (Tissue 1); 26.1 mg (Tissue 2)
1 h exposure: 26.6 mg (Tissue 1 and 2) - Duration of treatment / exposure:
- 3 minutes exposure
1 h exposure - Number of replicates:
- 2
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 3 minutes treatment
- Value:
- ca. 72.5
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: Inconclusive, to be determined based on 1 h reading
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 1 h treatment
- Value:
- ca. 10.7
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of irritation
- Other effects / acceptance of results:
- All Validity criteria for the Experiment were met:
1. The criterion for optical density of the negative control (≥ 0.8 and ≤ 2.8) was fulfilled: optical density was 2.1 (3 minutes); 2.0 (1 h).
2. The positive control showed clear corrosive effects. The criterion for the viability of the 1 h experiment, expressed as % of the negative control (< 15%), was fulfilled, too. The mean value of relative tissue viability was 9.9%.
3. Values for negative control and for positive control were within the range of historical data of the test facility
Any other information on results incl. tables
The mean value of relative tissue viability of the test substance was reduced to 72.5% after 3 minutes treatment. Per criteria for assessment of corrosivity, this value is above the threshold for corrosively (50%). After 1 h treatment, the mean value of relative tissue viability of the test substance was reduced to 10.7%, lying below the threshold for corrosively (15%). Therefore, the test substance is considered as corrosive to skin.
The values of the negative control met the required acceptability criterion of mean OD ≥ 0.8 and ≤ 2.8 for both treatment intervals, thus showing the quality of the tissues. The positive control has met the validity criterion too, thus ensuring the validity of the test system.
Absorbance values blank isopropanol (OD 570 nm) | |||||||
Replicate | 1 | 2 | 3 | 4 | 5 | 6 | Mean |
Absorbance | 0.039 | 0.038 | 0.039 | 0.04 | 0.038 | 0.038 | 0.039 |
Replicate | 7 | 8 | 9 | 10 | 11 | 12 | |
Absorbance | 0.04 | 0.038 | 0.043 | 0.038 | 0.039 | 0.038 |
Absorbance values (OD 570 nm) of negative control, test substance and positive control | ||||||
Incubation | Negative Control | Test substance | Positive Control | |||
Tissue 1 | Tissue 2 | Tissue 1 | Tissue 2 | Tissue 1 | Tissue 2 | |
3 min | 2.125 | 2.117 | 1.456 | 1.576 | 0.404 | 0.447 |
2.115 | 2.09 | 1.527 | 1.587 | 0.427 | 0.446 | |
2.147 | 2.093 | 1.527 | 1.593 | 0.423 | 0.446 | |
1 h | 2 | 1.974 | 0.25 | 0.248 | 0.26 | 0.224 |
2.093 | 2.009 | 0.259 | 0.25 | 0.244 | 0.221 | |
2.116 | 1.988 | 0.259 | 0.251 | 0.246 | 0.225 |
Mean Absorbance Values of the 3 Minutes Experiment | |||
Designation | Negative Control | Test substance | Positive Control |
Mean – blank (tissue 1) | 2.09 | 1.464 | 0.379 |
Mean – blank (tissue 2) | 2.061 | 1.546 | 0.407 |
Mean of the two tissues | 2.076 | 1.505 | 0.393 |
RSD | 1.00% | 3.90% | 5.10% |
Mean Absorbance Values of the 1 h Experiment | |||
Designation | Negative Control | Test substance | Positive Control |
Mean – blank (tissue 1) | 2.031 | 0.217 | 0.211 |
Mean – blank (tissue 2) | 1.951 | 0.211 | 0.184 |
Mean of the two tissues | 1.991 | 0.214 | 0.198 |
RSD | 2.80% | 2.10% | 9.50% |
Comparison of Tissue Viability | ||
Incubation | Positive Control | Test substance |
3 min | 18.90% | 72.50% |
1 h | 9.90% | 10.70% |
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (corrosive) based on GHS criteria
- Conclusions:
- Under the study conditions, the test substance was considered as corrosive to skin.
- Executive summary:
A study was conducted to determine skin corrosion potential of the test substance using the reconstructed human epidermis (RHE) test method according to OECD Guideline 431, in compliance with GLP. Two tissues of the human skin model EpiDermTM were treated with the test substance for 3 min and 1 h, respectively. Demineralised water was used as negative control and potassium hydroxide (8M) was used as positive control. After treatment, the respective substance was rinsed from the tissues. Then, cell viability of the tissues was evaluated by MTT, which can be reduced to a blue formazan. Formazan production was evaluated by measuring the optical density (OD) of the resulting solution. After treatment with the test substance, the mean value of relative tissue viability was reduced to 72.5% (3 min exposure) and 10.7% (1 h exposure), which qualifies as corrosive. Under the study conditions, the test substance was therefore considered as corrosive to skin (Andres, 2018).
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