Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 235-406-9 | CAS number: 12220-06-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December from 4th to 8th, 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- The study described was conducted in accordance with SN EN 45001. No analytical determinations were conducted.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 17 July, 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- adopted 31 July, 1992, published 29 December 1992
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: Guppy.
- Length at study initiation: 1 - 3 cm
- Breeding: parental and young fish population was held in two 250 l glass aquaria. Illumination 14h a day and temperature of 23 ± 2 °C. The medium was continuously aerated and filtered tap water from Schlieren. The bottom of the aquaria was covered with gravel (<1.5 cm). Every month, one third of the total volume of the aquaria was replaced by fresh tap water.
- Feeding during test: fish were last fed 24 h before the start of the definitive test.
- Food type: sera micropan, Sera vipan (Sera apanristic GmbH, D-52525 Heinsberg, Germany) daily.
- Health during acclimation: during 7 days before the definitive test, mortality in the batch of which fish were used for the test was less than 5 %.
ACCLIMATION
- Acclimation period: to start the test fish were transferred into the test vessels about 1 h after formation of the test media. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 21 ± 1 °C
- pH:
- Control: 8.6 - 8.9
Test vessels: 7.5 - 8.8 - Dissolved oxygen:
- Control: 7.4 - 7.9 mg/l
Test vessels: 7.3 - 7.8 mg/l - Nominal and measured concentrations:
- 100 mg/l nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 l aquaria, all-glass.
- Aeration: aerated.
- No. of organisms: 7 fish per test concentration.
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: tap water from Schlieren. At least 1 h before the test the test vessels were filled with the medium to allow equilibration of pH, oxygen and temperature.
- pH: 7.1 - 7.4
- Conductivitv (20 "C): 510 - 585 µS/cm
- CO3 Hardness: 15.3 - 27.8 °F
- CO3 Hardness after Heyer: 15.2 - 26.9 °F
- Rest Hardness: 2.4 - 4.1 °F
- Total Hardness: 17.7 - 31.9 °F
- KMnO4-consumotion: 1.0 - 1.3 mg/l
- E. Coli (at 44 °C): 0 % ml
- Colony count: 0 % per ml at 20 "C; 0- 5 % per ml at 30 °C.
- Chloride: 7.5 - 12.0 mg/l
- Sulfate: 17.5 - 21.0 mg/l
- Phosphate as PO4-P: <0.002 - 0.038 mg/l
- Ammonia as NH4-N: <0.01 mg/l
- Nitrite as NO2-N: <0.002 mg/l
- Nitrate as NO3-N: 7.0 - 12.0 mg/l
OTHER TEST CONDITIONS
- Adjustment of pH: none.
- Photoperiod: 16 h photoperiod a day, supplied by overhead white fluorescent tubes.
EFFECT PARAMETERS MEASURED
Fish were considered to be dead when they showed no reaction after touching the caudal fin or no visible breathing movements.
Based on the binominal theory, the absence of mortality of a total of seven fish provides at least 99 % confidence that the LC50 is greater than the applied nominal test concentration of 100 mg/|.
MEASUREMENTS
- Oxygen, pH, temperature: prior to the addition of fish to the test medium and after 2, 24, 48, 72 and 96 h of exposure.
- Observations: observations of the fish were made after 2, 24, 48, 72 and 96 h of exposure. Moftality of the fish was recorded at the same time intervals. Dead fish were removed immediately from the test medium and weight and length recorded. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- At the nominal concentration of 100 mg/l none of a total of seven fish died after 96 h of exposure. Other toxic effects than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed.
The no-observed-effect concentration (NOEC) was higer than 100 mg/l.
No mortality was also observed in the blank control. - Sublethal observations / clinical signs:
Data of surviving fish after termination of the test.
Nominal concentration (mg/l) 100 length (mm) 22 21 20 23 24 19 13 weight (mg) 88 68 79 98 101 43 32 - Validity criteria fulfilled:
- not specified
- Remarks:
- no chemical analysis of the test substance concentration was conducted
- Conclusions:
- LC50 (96h) > 100 mg/l (nominal)
- Executive summary:
The acute lethal toxicity of the test substance to Poecilia reticulata (Guppy) was investigated under static exposure conditions over a period of 96 hours, following the OECD guideline 203. The only applied nominal test concentration was 100 mg/l. No chemical analysis of the test substance concentration was conducted.
At the nominal concentration of 100 mg/l none of a total of seven fish died after 96 hours of exposure. No mortality was also observed in the blank control.
Other toxic effects than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed.
The no-observed-effect concentration (NOEC) was higher than 100 mg/l.
Conclusion
LC50 (96h) > 100 mg/l (nominal)
Reference
Description of key information
LC50 (96h) > 100 mg/l (nominal)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The acute lethal toxicity of the test substance to fish was investigated under static exposure conditions over a period of 96 hours, following the OECD guideline 203. The only applied nominal test concentration was 100 mg/l. No mortality was also observed in the blank control. At the nominal concentration of 100 mg/l none of a total of seven fish died after 96 hours of exposure; other toxic effects than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed, thus the No-Observed-Effect Concentration (NOEC) resulted to be higher than 100 mg/l.
Although no chemical analysis was conducted, the test item is expected to be stable under the tested conditions and over the exposure period: Acid Orange 067 does not undergo to a rapid biotic neither abiotic transformation processes, which may impact the stability during the test.
Data regarding an old test are also available and indicate a LC50 value at 48 hours of 100 mg/l. There are no details on testing method and procedures followed; only the sheet of results is available (reference into CSR).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.