Pyrazole

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

guideline study (no analytics, no GLP)

Qualifier:

equivalent or similar to guideline

Guideline:

other: DIN 38 412

GLP compliance:

no

Analytical monitoring:

no

Details on test solutions:

The test solution was prepared by adding the test item to the test media without any pre-treatment. Then, the fish were placed into the test aquaria.

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe (Leuciscus idus)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range): 8.4 cm (range 7.5 - 9.3 cm)
- Weight at study initiation (mean and range): 6.0 g (range 4.4 - 7.6 g)
- Feeding during test: Withdrawal of food one day before and during exposure

ACCLIMATION
- Health during acclimation (any mortality observed): 0%

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 mmol/L

Test temperature:

20 to 21°C

pH:

7.1 to 7.5

Dissolved oxygen:

7.9 to 8.7 mg/L

Nominal and measured concentrations:

0, 1000, 2150, 4640 and 10000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: All glass aquaria (30 x 22 x 24 cm), Volume of water: 10 L
- Aeration: Continuous aeration with oil-free air, slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 6 g fish/L test water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982
- Alkalinity: pH about 8.0
- Ca/Mg ratio: 4 : 1

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
observations: mortality and symptoms after 1, 4, 24, 48, 72, and 96 h
measurements: pH and oxygen content after 1, 24, 48, 72 and 96 h

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 2 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Behavioural abnormalities: Symptoms of intoxication: Narcotic-like state, Tumbling [LOEC: 2150 mg/L]

Results with reference substance (positive control):

- LC50: about 31 mg/L (after 48 h)

Validity criteria fulfilled:

yes

Conclusions:

96-hour LC50 (Leuciscus idus): ca. 2200 mg/L

Executive summary:

The acute toxicity of the test substance to fish was studied in a method equivalent to DIN 38 412. Leuciscus idus were exposed to the test substance at concentrations of 1000, 2150, 4640, and 10000 mg/L. The 96-hour LC50 was ca. 2200 mg/L.

Description of key information

96-hour LC50 (Leuciscus idus): ca. 2200 mg/L; DIN 38 412; Reliability = 2

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

2 200 mg/L