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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study. Limitations in design and/or reporting but outcome considered acceptable for assessment.
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION:
A stock solution of 1250 mg/L was prepared by stirring the test substance in deionised water.
Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Laboratory culture: The used test strain of Pseudomonas putida DSM 50026 is purchased on regular times from DSM (Deutsche Sammlung von Mikroorganismen in Braunschweig / German collection of microorganisms in Braunschweig) and cultured further on agar slants in the Laboratory of Ecotoxicology of BASF AG in Ludwigshafen, Germany.
- Pretreatment: The pre-cultures are kept in Erlenmeyer flasks with a nominal volume of 300 mL. The vessels are filled with 100 mL pre-culture medium and inoculated with a smear from an inoculation loop with 10 TE/F bacteria suspension at 24 ± 1 °C and incubated for 7 h.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Remarks on exposure duration:
according to guideline
Test temperature:
20 °C
Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 7.81, 15.63, 32.25, 62.5, 125, 250, 500 and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: closed tubes of glass with flat bottom (nominal volume: 50 mL, fill volume: 10 mL)

TEST MEDIUM / WATER PARAMETERS
- Test medium: AK-Medium according to DIN 38412, Part 8

EFFECT PARAMETERS MEASURED
- Optical density at a wavelength of 436 nm. Measurements are made at t=16h in all inoculated replicates, whereby the uninoculated replicate of each concentration is used as background measurement.
Reference substance (positive control):
no
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
567.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks:
(inhibition of cell multiplication)
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
933.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks:
(inhibition of cell multiplication)
Duration:
16 h
Dose descriptor:
other: EC90
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks:
(inhibition of cell multiplication)

Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
933.2 mg/L

Additional information