6,10-dimethylundecan-2-one

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6,10-dimethylundecan-2-one

EC Number:

216-509-8

EC Name:

6,10-dimethylundecan-2-one

Cas Number:

1604-34-8

Molecular formula:

C13H26O

IUPAC Name:

6,10-dimethylundecan-2-one

Details on test material:

- Name of test material (as cited in study report): Tetrahydrogeranylaceton
- Lot/batch No.: B 633
- Analytical purity: 97.5%
water solubility: < 80 mg/L (Laboratory observation).

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Activated sludge from Iaboratory wastewater plant treating municipal sewage. Concentration of dry substance 30
mg/I.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

26 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

other: TOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: according to OECD 301
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 +/- 2 °C
- pH: 7.3 - 7.5
- pH adjusted: yes
- Suspended solids concentration: 6 g/L dry matter
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: stirring
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency: variable
- Sample storage before analysis: no

CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Toxicity control: 1


STATISTICAL METHODS: not applicable

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

0 - 10

Sampling time:

3 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

10 - 20

Sampling time:

5 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

50 - 60

Sampling time:

17 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

70 - 80

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Kinetic of control substance (in %):
day 3: 0 - 10 %
day 5: 10 - 20 %
day 14: 60 - 70 %
day 28: 90 %

Applicant's summary and conclusion

Interpretation of results:

other: CO2 of ThCO2; biodegradable, not readily biodegradable according to OECD criteria;