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Diss Factsheets
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EC number: 241-997-4 | CAS number: 18096-62-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 - 24 Jul 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Gewerbeaufsicht in Niedersachsen, Staatliches Gewerbeaufsichtsamt Hildesheim, Germany
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 100 mg/L
- Sampling method: Samples were taken at 0 and 96 h.
- Sample storage conditions before analysis: All original samples were stored at 6 ± 2 °C. Prepared samples were stored at room temperature in the autosampler until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item amount was weighed and applied to the test media in the aquaria. The test media were mixed with an ultraturrax (3 min, 17000 rpm) and aerated for 30 min.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: Originally supplied from Umweltbundesamt, Berlin, Germany.
- Length at study initiation (length definition, mean, range and SD): 2.24 cm (2.2 - 2.3 cm)
- Weight at study initiation (mean and range, SD): 0.183 g (measurement of all fish together)
- Feeding during test: none
ACCLIMATION
- Acclimation period: 12 d
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Sera vipan, SERA GmbH, Heinsberg, Germany; 4% of fish body weight
- Feeding frequency: 3 times per week; fish were not fed 24 h before start
- Health during acclimation (any mortality observed): Mortality < 5% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 56.1 mg CaCO3/L (control)
- Test temperature:
- 22.6 - 23.2 °C
- pH:
- 7.31 - 7.57
- Dissolved oxygen:
- 97 - 100%
- Nominal and measured concentrations:
- nominal: control, 100 mg/L
measured (0 h): < LOQ, 89.7 mg/L
measured (96 h): < LOQ, 91.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria
- Material, size, headspace, fill volume: glass, 25.5/18/28 cm (13 L), headspace: 3 L, fill volume: 10 mL
- Aeration: yes
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.128 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH-value, temperature and oxygen saturation were measured in all test vessels at the start of the exposure and every 24 hours. Total hardness was determined at the start of the exposure out of the control. During the test the water temperature was recorded continuously (once per hour) with a data logger. The light intensity on the surface of the test aquaria was measured at the start of the exposure.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
- Light intensity: 7-750 lux
EFFECT PARAMETERS MEASURED
- Mortality: Mortality was recorded after 2, 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Range finding study: no - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Sublethal observations / clinical signs:
All effect concentrations were based on the nominal concentrations since the measured concentrations were in the range of > 80 - < 120% of nominal (90 and 91%).
- Validity criteria fulfilled:
- yes
- Conclusions:
- No mortality of Danio rerio was observed at the limit concentration of 100 mg/L in a guideline study according to OECD 203 (GLP). Hence, the LC50 (96 h) was derived to be > 100 mg/L (nominal).
- Executive summary:
The study was performed according to OECD 203 (GLP). Danio rerio was exposed to a limit concentration of 100 mg/L (nominal) under static conditions. The exposure concentrations were verified by HPLC-DAD analysis and resulted in 90% (0 h) and 91% (96 h) of nominal. Thus, the effect concentrations were based on the nominal concentrations. After a period of 96 h no mortality was recorded in the control and exposure vessels. Hence, the LC50 (96 h) was derived to be > 100 mg/L. All validity criteria were met.
Reference
Description of key information
No mortality of Danio rerio was observed at a nominal concentration of 100 mg/L in a guideline study according to OECD 203 (GLP). Hence, the LC50 (96 h) was derived to be > 100 mg/L (nominal).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
One experimental study is available investigating the short-term toxicity of the test substance to fish (2015). The study was performed according to OECD 203 (GLP). Danio rerio was exposed to a limit concentration of 100 mg/L (nominal) under static conditions. The exposure concentrations were verified by HPLC-DAD analysis and resulted in 90% (0 h) and 91% (96 h) of nominal. Thus, the effect concentrations were based on the nominal concentrations. After a period of 96 h no mortality was recorded in the control and exposure vessels. Hence, the LC50 (96 h) was derived to be > 100 mg/L. All validity criteria were met.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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