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EC number: 235-920-3 | CAS number: 13047-13-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 October 2017 to 23 November 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, 2000.
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-(hydroxymethyl)-4-methyl-1-phenylpyrazolidin-3-one
- EC Number:
- 235-920-3
- EC Name:
- 4-(hydroxymethyl)-4-methyl-1-phenylpyrazolidin-3-one
- Cas Number:
- 13047-13-7
- Molecular formula:
- C11H14N2O2
- IUPAC Name:
- 4-(hydroxymethyl)-4-methyl-1-phenylpyrazolidin-3-one
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations at 0 and 24 h (fresh) and at 24 and 48 h (old) --> 2.0 mL from the approximate centre of the test vessels
- Sample storage conditions before analysis: anaylsed freshly
- sample preparation:diluted in a 1:1 (v:v) ratio with 1% L-ascorbic acid in ISO-medium and analyzed ( the substance is known to be stable in 0.5% L-ascorbic acid in water)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Preparation of test solutions started with the highest concentration of 100 mg/L with 21 minutes of magnetic stirring to accelerate dissolution of the test item in medium. Lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. At the end of the preparation procedure, all test solutions were clear and differed in color from very light yellow/brown to yellow/brown with increasing test concentration.
Due to the light sensitive nature of the test item, preparation was performed under dimmed light and the glassware was wrapped in aluminum foil.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus, 1820
- Source: in-house laboratory culture
- Age of parental stock (mean and range, SD): < 24 H
- Feeding during test: none
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L as CaCO3
- Test temperature:
- 20 - 21°C
- pH:
- 7.7-8.1
- Dissolved oxygen:
- 7.3-10 mg/L
- Salinity:
- NA
- Nominal and measured concentrations:
- Nominal: 2.2, 4.6, 10, 22, 46 and 100 mg/L
measured (TWA) : 0.85, 1.7, 3.8, 8.4, 20 and 44 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 60 mL glass with 50 mL test solution
- Aeration: No
- Renewall of test solution: at 24 h:
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS: according to the guideline (adjusted ISO medium)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: in dark
EFFECT PARAMETERS MEASURED: immobility at 24 and 48 hours
RANGE-FINDING STUDY
- Test concentrations: 0, 0.10, 1.0 and 10 mg/L
- Results used to determine the conditions for the definitive study: immobility at 48 h 0, 10, 60 and 100% at 0, 0.10, 1.0 and 10 mg/L - Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50 of potassium dichromate 0.41 mg/L with a 95% confidence interval between 0.34 and 0.46 mg/L
- Reported statistics and error estimates:
- 95% confidence interval substance EC50 3.2 – 5.1 mg/L
Probit analysis with ToxRat Professional v 3.2.1 (ToxRat Solutions® GmbH, Germany)
Any other information on results incl. tables
Immobility
Time (h) |
Replicate |
CH01610; Average exposure concentration (mg/L) |
||||||
Control |
0.85 |
1.7 |
3.8 |
8.4 |
20 |
44 |
||
0 |
A |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
B |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
C |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
D |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
Total introduced |
20 |
20 |
20 |
20 |
20 |
20 |
20 |
|
|
||||||||
24 |
A |
0 |
0 |
0 |
1 |
2 |
2 |
5 |
B |
0 |
0 |
0 |
1 |
3 |
4 |
5 |
|
C |
0 |
0 |
0 |
1 |
3 |
4 |
5 |
|
D |
0 |
0 |
0 |
0 |
3 |
4 |
5 |
|
Total immobilised |
0 |
0 |
0 |
3 |
11 |
14 |
20 |
|
Effect % |
0 |
0 |
0 |
15 |
55 |
70 |
100 |
|
|
|
|
|
|
|
|
||
48 |
A |
0 |
0 |
1 |
4 |
4 |
5 |
n.d. |
B |
0 |
0 |
0 |
2 |
5 |
5 |
n.d. |
|
C |
0 |
0 |
0 |
2 |
4 |
5 |
n.d. |
|
D |
0 |
0 |
0 |
4 |
3 |
5 |
n.d. |
|
Total immobilised |
0 |
0 |
1 |
12 |
16 |
20 |
n.a. |
|
Effect % |
0 |
0 |
5 |
60 |
80 |
100 |
n.a. |
sample analyses
CH01610 |
Measured concentrations (mg/L) |
Average exposure |
|||
t=0h (fresh) |
t=24h (old) |
t=24h (fresh) |
t=48h (old) |
||
2.2 |
1.8 |
0.39 |
1.7 |
0.42 |
0.85 |
4.6 |
3.5 |
0.67 |
3.7 |
0.85 |
1.7 |
10 |
8.2 |
1.5 |
8.5 |
2.1 |
3.8 |
22 |
18 |
3.5 |
18 |
4.4 |
8.4 |
46 |
38 |
8.6 |
39 |
11 |
20 |
100 |
83 |
23 |
n.d. |
n.d. |
441 |
n.d. – not determined,1 calculated based only on the results obtained in the first renewal, i.e. as mathematical means of the concentrations of the substance measured in the samples taken at the start (Ct=0), after 24 hours in old (Ct=24, old) and fresh medium (Ct=24, fresh) and at the end of the test (Ct=48).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 h EC50 of the substance for daphnia magna was 4 mg/L (based on measured concentrations)
- Executive summary:
In a test according to OECD 202 with a semi-static designe (renewal after 24 h), daphnids were exposed to the test substance at 2.2, 4.6, 10, 22, 46 and 100 mg/L (nominal) for 48 hours. Analyses of the fresh and old solutions showed that concentrations dropped to 18 -29% of initial. The time weight average concentrations were 0.85, 1.7, 3.8, 8.4, 20 and 44 mg/L. Immobilisation was observed at 3.8 mg/L and above. The EC50 was 4.0 mg/L.
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