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EC number: 408-090-7 | CAS number: 100418-33-5 METHYLGELB
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-((4-methyl-2-nitrophenyl)amino)ethanol
- EC Number:
- 408-090-7
- EC Name:
- 2-((4-methyl-2-nitrophenyl)amino)ethanol
- Cas Number:
- 100418-33-5
- Molecular formula:
- C9H12N2O3
- IUPAC Name:
- 2-[(4-methyl-2-nitrophenyl)amino]ethan-1-ol
- Test material form:
- solid: crystalline
- Details on test material:
- red powder
Batch # : BRA1/285
Constituent 1
Test animals
- Species:
- other: rat and mouse
- Strain:
- other: Wistar rats, CF1 mice
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 10 % gummi arabicum solution
- Doses:
- Rats: 900, 1700 and 2500 mg/kg bw
Mice: 1000, 1500, 2000 and 2500 mg/kg bw - No. of animals per sex per dose:
- Rats: 6 animals per sex and dose
Mice: 10 animals per sex and dose
Results and discussion
Effect levelsopen allclose all
- Sex:
- female
- Dose descriptor:
- LD50
- Remarks:
- rat
- Effect level:
- 1 436 mg/kg bw
- Based on:
- test mat.
- Sex:
- male
- Dose descriptor:
- LD50
- Remarks:
- rat
- Effect level:
- 1 564 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Remarks:
- mouse
- Effect level:
- 1 750 mg/kg bw
- Based on:
- test mat.
- Sex:
- male
- Dose descriptor:
- LD50
- Remarks:
- mouse
- Effect level:
- 1 600 mg/kg bw
- Based on:
- test mat.
Any other information on results incl. tables
Table 1: Mortality rates in rats and mice after a single oral application:
Species | Dose [mg/kg bw] | |||||
900 | 1000 | 1500 | 1700 | 2000 | 25000 | |
Mice (female) | n.t. | 0/10 | 4/10 | n.t. | 6/10 | 10/10 |
Mice (male) | n.t. | 2/10 | 4/10 | n.t. | 7/10 | 10/10 |
Rats (female) | 1/6 | n.t. | n.t. | 3/6 | n.t. | 6/6 |
Rats (male) | 2/6 | n.t. | n.t. | 3/6 | n.t. | 6/6 |
n.t. = not tested
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The above described study allows a formally valid classification in terms of the acute oral toxicity of Hydroxyethyl-2-nitro-p-toluidine. According to the results obtained, Hydroxyethyl-2-nitro-p-toluidine is harmful if swallowed.
- Executive summary:
The test substance, suspended in a 10 % gummi arabicum solution, was administered at 3 dose levels from 900 to 2500 mg/kg bw to rats and at 4 dose levels from 1000 to 2500 mg/kg bw to mice once by oral gavage. Mortality and clinical signs were checked daily for the 14-day observation period. Body weights were recorded weekly and all animals were submitted to a gross necropsy at the end of the observation period. Doses tested in the main study were based on a pretest in female mice (3 per test doses of 1, 2 and 4 g/kg bw), which revealed a median lethal dose between 1000 and 2000 mg/kg bw. The only clinical signs reported were reduced activity in both rats and mice. In both species the urine and extremities were coloured orange up to 24h after administration. Based on the observed mortality rates, the following LD50 figures were calculated by the method of Spearman-Kärber:
LD50 rat ♀: 1436 mg/kg bw
LD50 rat ♂: 1564 mg/kg bw
LD50 mouse ♀: 1750 mg/kg bw
LD50 mouse ♂: 1600 mg/kg bw
The available acute oral toxicity study was not performed according to the respective OECD guideline (OECD 401) but according to the recommendations by the FDA in place at that time. The study fulfils the formal GLP requirements. The investigations/data recorded are in line with or exceed the requirements described in OECD 401, e.g. with regard to number of animals in the study with rats. Furthermore a second rodent species (mouse) was also investigated. The study is therefore considered as valid without restrictions and allows a scientifically sound evaluation of the acute oral toxicity of HYDROXYETHYL-2-NITRO-p- TOLUIDINE
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