3,4-dihydroxybenzonitrile

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Version / remarks:

May 31, 2008

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Version / remarks:

January 22, 2001

Guideline:

other: EC Technical Guidance Document on Risk Assessment Part III Chapter 4.3: Use of (Quantitative) Structure Activity Relationships ((Q)SARs) - Soil and Sediment Sorption.

Version / remarks:

2003

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil

Radiolabelling:

no

Details on study design: HPLC method:

EQUIPMENT
Instrument Acquity UPLC system
(Waters, Milford, MA, USA)
Detector Acquity UPLC TUV detector (Waters)
Column Acquity UPLC HSS Cyano,
100 mm 2.1 mm i.d., dp = 1.8 µm (Waters)
Column temperature 35°C 1°C
Injection volume 5 µL
Mobile phase 55/45 (v/v) methanol/0.01M buffer pH 5.5
Flow 0.4 mL/min
UV detection 210 nm

MOBILE PHASES
A stock solution of the reference substance at a concentration of approximately 1 g/L in methanol were used. The stock solution was diluted to obtain an end solution of 55/45 (v/v) methanol/0.01M buffer pH 5.5.
A 1000 mg/L stock solution of the test item was prepared in methanol. The stock solution was diluted to obtain an end solution of 55/45 (v/v) methanol/0.01M buffer pH 5.5. The final concentration of the test item solution was 50.0 mg/L.

REFERENCE SUBSTANCES
- Identity: Acetanilide

REPETITIONS
Reference substance and test item solutions were injected in duplicate. Blank solutions were analyzed by single injection.

Type:

Koc

Value:

< 18.2 dimensionless

pH:

5.5

Type:

log Koc

Value:

< 1.26 dimensionless

pH:

5.5

Details on results (HPLC method):

UPLC-UV chromatograms of the test item solution and the corresponding blank are shown in Figure 6 and Figure 7, respectively. In the chromatogram of the test item solution, one peak with a retention time of 0.862 minutes was observed. Under the same analytical conditions, the retention time of acetanilide (log Koc = 1.26) was 0.902 minutes. It was concluded that the log Koc of test item was < 1.26 (Koc < 1.82 101).

Validity criteria fulfilled:

yes

Conclusions:

It was concluded that the log Koc of test item was < 1.26 (Koc < 1.82 * 10^1).

Key value for chemical safety assessment

Koc at 20 °C:

18.2