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EC number: 201-803-0 | CAS number: 88-14-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard OECD test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared by dissolving 100mg of test chemical in 100ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively. Test chemical concentrations used for the study were 0, 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l and 100 mg/l, respectively.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Feeding during test: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 155.5 mg of CaCO3
- Test temperature:
- 20±2°C
- pH:
- 7.1
- Nominal and measured concentrations:
- Nominal concentrations: 0, 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l and 100 mg/l, respectively.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
- Light intensity: 1000 – 1500 Lux - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
- Conclusions:
- Based on nominal concentrations, experimental median effective concentrations [EC50 (48 h)] for test chemical on Daphnia magna was determined to be > 100 mg/L.
- Executive summary:
An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test solution was prepared by dissolving 100mg of test chemical in 100ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively. Test chemical concentrations used for the study were 0, 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l and 100 mg/l, respectively. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions with light intensity 1000 – 1500 Lux, respectively. One control vessel was also run simultaneously during the study. The animals in control and test chemical concentrations were exposed for a period of 48 hour. No Immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be >100 mg/L. Thus, based on the EC50 value, chemical was considered as non-toxic to aquatic invertebrates at environmental relevant concentrations and hence, considered to be 'not classified' as per CLP classification criteria.
Reference
Observations:
Table: Immobility
Nominal Concentration (mg/l) |
Number of Daphnids |
Immobility |
||
0 h |
24 h |
48 h |
||
Control |
10 |
- |
- |
- |
6.25 |
10 |
- |
- |
- |
12.5 |
10 |
- |
- |
- |
25 |
10 |
- |
- |
- |
50 |
10 |
- |
- |
- |
100 |
10 |
- |
- |
- |
Table: pH and oxygen content
Nominal Concentration (mg/l) |
Number of Daphnids |
pH (mg/l) |
|
0 h |
48 h |
||
Control |
10 |
7.5 |
7.7 |
6.25 |
10 |
7.6 |
7.8 |
12.5 |
10 |
7.6 |
7.8 |
25 |
10 |
7.7 |
7.9 |
50 |
10 |
7.7 |
7.9 |
100 |
10 |
7.7 |
7.9 |
Nominal Concentration (mg/l) |
Number of Daphnids |
DO (mg/l) |
|
0 h |
48 h |
||
Control |
10 |
7.0 |
6.8 |
6.25 |
10 |
7.0 |
6.7 |
12.5 |
10 |
7.0 |
6.7 |
25 |
10 |
7.0 |
6.7 |
50 |
10 |
7.0 |
6.6 |
100 |
10 |
7.0 |
6.6 |
Table: Test temperature
Nominal Concentration (mg/l) |
Number of Daphnids |
Temparature |
|
0 h |
48 h |
||
Control |
10 |
21.2 |
21.5 |
6.25 |
10 |
21.2 |
21.5 |
12.5 |
10 |
21.2 |
21.5 |
25 |
10 |
21.2 |
21.5 |
50 |
10 |
21.2 |
21.5 |
100 |
10 |
21.2 |
21.5 |
Description of key information
An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna (Study report). The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test solution was prepared by dissolving 100mg of test chemical in 100ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively. Test chemical concentrations used for the study were 0, 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l and 100 mg/l, respectively. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions with light intensity 1000 – 1500 Lux, respectively. One control vessel was also run simultaneously during the study. The animals in control and test chemical concentrations were exposed for a period of 48 hour. No Immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be >100 mg/L. Thus, based on the EC50 value, chemical was considered as non-toxic to aquatic invertebrates at environmental relevant concentrations and hence, considered to be 'not classified' as per CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Experimental study of the test chemical and various supporting weight of evidence studies for its read across chemical were reviewed for short term toxicity to aquatic invertebrate end point which are summarized as below:
In an experimental study from study report, an acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test solution was prepared by dissolving 100mg of test chemical in 100ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively. Test chemical concentrations used for the study were 0, 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l and 100 mg/l, respectively. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions with light intensity 1000 – 1500 Lux, respectively. One control vessel was also run simultaneously during the study. The animals in control and test chemical concentrations were exposed for a period of 48 hour. No Immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be >100 mg/L. Thus, based on the EC50 value, chemical was considered as non-toxic to aquatic invertebrates at environmental relevant concentrations and hence, considered to be 'not classified' as per CLP classification criteria.
In a supporting weight of evidence study, short term toxicity to aquatic invertebrate study was conducted for 48 hrs for assessing the effect of test chemical (authoritative database, 2018). The study was performed under static conditions using Daphnia magna (Water flea) as a test organism. On the basis of the effect of test chemical on mobility of the test organism Daphnia magna, the 48 hr median effect concentration (EC50) value was determined to be 27 mg/l. Thus, test chemical was considered as toxic to aquatic invertebrates at environmental relevant concentrations. Since, the test chemical is readily biodegradable in water, chemical was considered as non-toxic and hence, considered to ‘not classified’ as per the CLP classification criteria.
Another short term toxicity to aquatic invertebrate study was conducted for 48 hrs for assessing the effect of test chemical. The study was performed following the principles of the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) under static conditions using Daphnia magna (Water flea) as a test organism. On the basis of the effect of chemical on mobility of the test organism Daphnia magna, the 48 median effect concentration (EC50) value was determined to be 110 mg/l. Thus, test chemical was considered as non-toxic to aquatic invertebrates at environmental relevant concentrations and hence, considered to be ‘not classified’ as per the CLP classification criteria.
On the basis of the above results, it can be concluded that the test chemical was considered to be toxic to aquatic invertebrates. Since, the test chemical is readily biodegradable in water, chemical was considered as non-toxic to aquatic invertebrates and hence, considered to be not classified as per the CLP classification criteria.
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