1-chloro-2-(dichloromethyl)benzene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2-(dichloromethyl)benzene

EC Number:

201-849-1

EC Name:

1-chloro-2-(dichloromethyl)benzene

Cas Number:

88-66-4

Molecular formula:

C7H5Cl3

IUPAC Name:

1-chloro-2-(dichloromethyl)benzene

Details on test material:

Purity > 99%

Method

Target gene:

In the Salmonella typhimurium strains (TA 1535, TA 100, TA 1537, TA 1538, TA 98) the amino acid histidine locus is the target gene.
In the Escherichia coli strain (WP2 uvrA) the amino acid tryptophan locus is the target gene.

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

Rat liver S-9 mix from Arochlor 1254 induced rats

Test concentrations with justification for top dose:

0, 0.8, 4, 20, 100, 500, 2500 µg/plate

Controls

Untreated negative controls:

yes

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Applicant's summary and conclusion

Conclusions:

o-Chlorobenzal chloride is not mitagenic in these bacterial test systems either with or without exogenous metabolic activation at the does levels investigated.

Executive summary:

o-Chlorobenzal chloride was tested for mutagenicity with the strains TA 100, TA 1535, TA 1537, TA 1538, TA 98 of Salmonella typhimurium and on Escherichia coli WP2uvrA.

The mutagenicity studies were conducted in the absence and in the presence of a metabolizing system derived from rat liver homogenate. A dose range of 6 different doses from 0.8 µg/plate to 2500 µg/plate was used.

Control plates without mutagen showed that the number of spontaneous revertant colonies was similar to that described in the literature. All positive control compounds gave the expected increase in the number of revertant colonies.

Toxicity: The test compound proved to be toxic to most of the bacterial strains at 100 µg/plate. 2500 µg/plate was chosen as top dose level for the mutagenetic study.

Mutagenicity: In the absence of the metabolic activation system the test compound did not show a dose dependent increase in the number of revertants in any of the bacterial strains. Also in the presence of a metabolic activation system, treatment of the cells with o-chlorobenzal chloride did not result in relevant increases in the number of revertant colonies.

Summarizing, it can be stated that o-chlorobenzal chloride is not mutagenic in these bacterial test system either with or without exogenous metabolic activation at the does levels investigated.