6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

This represents a deviation of the study plan which is not considered to have an influence on the integrity of the study

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione

EC Number:

249-682-3

EC Name:

6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione

Cas Number:

29529-99-5

Molecular formula:

C11H20N4S2

IUPAC Name:

6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Specimens were drawn from freshly prepared treatments and those incubated for 72 hr. The supporting analysis of the specimens could not be performed on the same day of collection of the specimens. Therefore, all specimens from freshly prepared test solutions and those incubated for 72hrs were stored deep-frozen at ≤ -18°C in glass bottles and analysed after thawing. Before analysis the treatments were diluted as follows: 10 mg/L specimens werde diluted 1:10, 20 mg/L specimens were diluted 1:20, 40 mg/L specimens were diluted 1:40, 80 mg/L and 160 mg/L specimens were diluted 1:100 using a mixture of 900 mL 0.2 mM ammoniumacetate + 100 mL acetonitrile being spiked 0.1% with formic acid

Test solutions

Vehicle:

not specified

Details on test solutions:

The screening test was performed using concentration steps 1 mg/L, 10 mg/L, and 100 mg/L dilution water. The test item was introduced into the ELENDT M4 dilution water whilst shaking on a shaking machine for 24 hr. Thereafter the solutions were filtrated through glass fibre filters and membrane filters in order to eliminate non dissolved parts of the test item. The prepared test solutions (filtrates/eluates) were filled into two test vessels using 5 animals each parallel. The daphnia were not fed during the test. Using a spacing factor of 2.0, the main test was performed accordingly using 10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L and 160 mg/L test item. The test solutions were filled into four test vessels using 5 animals each parallel. The daphnia were not fed during the test

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS (clone 5, obtained from Dr. M. Bergtold, BASF, D-67117 Limburgerhof; the strain originally derived from the Institut National de Recherche Chimique Appliquée, France in 1978) of an SGS INSTITUT FRESENIUS laboratory breed cultivated in medium M4 acc. ELENDT was used for the test. In order to verify the sensitivity of the test system towards toxic items the effect of potassium dichromate towards different animals of this breed was tested prior to beginning of the test. The value of the EC50 (24 hr) in this period of time was at 0.97 mg/L potassium dichromate. This value is in accordance with the recommendations of 0.6 to 2.1 mg/L K2Cr2O7 given in OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test). The cultivation of the daphnia is performed in a way that the animals are transferred in new test medium (see below) at an interval of 1 to 3 days followed by feeding with Desmodesmus subspicatus- and/or Chlorella-cells. Daphnias used for the test are not older than 24 hr. Therefore, in every case, daphnia are transferred into fresh medium one day before starting of the daphnia test in order to get daphnia for the test younger than 24 hr. During the test the daphnia were not fed.

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

The daphnia are exposed to different concentrations of the test item for 48 hr at 20 ± 1°C with a light-dark cycle. After 24 hr and 48 hr the number of immobilised daphnia at each concentration of the test item is recorded.

Post exposure observation period:

not specified

Test conditions

Hardness:

not specified

Test temperature:

20.4- 20.9

pH:

6.72 - 8.98

Dissolved oxygen:

7.1-7.4[mg O2/L]

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

Nominal Concentration of Test Item t0 mg/L t48hr % of t0 Mean*) measured Concentration of
6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione (“WAF”) Test Item (“WAF”) Test Item [mg/L]
[mg/L] mg/L Test Item(“WAF”)


0 0 0 - 0
10 9.921 10.088 101.7 10.004
20 20.176 20.260 100.4 20.218
40 40.186 40.130 99.9 40.158
80 81.271 84.053 103.4 82.650
160 166.944 1 69.169 101.3 168.053

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

94 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Basis: Nominal Concentrations
[mg 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione /L] (“WAF”)
t24hr t48hr
NOEL 80.0 80.0
LOEL 160.0 160.0
EL10 50.1 27.9
EL20 85.3 42.4
EL50 236.2 94.0

Any other information on results incl. tables

6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione was tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 (version dated 13-Apr-2004). In order to investigate the influence of the test item towards the daphnias, the swimming behaviour of the animals was recorded. The test was performed using five concentrations of “WAFs” (Water accommodated fractions) in the nominal range of 10 to 160 mg/L of 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione.Under the conditions used for the test, there was an immobilisation of the daphnia at concentrations ≥ 10 mg 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione/L. As a conclusion of the analytical part of this study, it can be stated that the concentrations 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione remained sufficiently stable during incubation of 48hr.As a result of the supporting analyse it can be stated that the results of the biological part should be based on the nominal concentrations applied because the recoveries of the nominal concentrations were all >80%.The followingEL-values (“Effective Loading”) were obtained:

	Basis: Nominal Concentrations [mg6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione/L](“WAF”)
	t24hr	t48hr
NOEL	80.0	80.0
LOEL	160.0	160.0
EL10	50.1	27.9
EL20	85.3	42.4
EL50	236.2	94.0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Basis: Nominal Concentrations
[mg 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione /L] (“WAF”)
t24hr t48hr
NOEL 80.0 80.0
LOEL 160.0 160.0
EL10 50.1 27.9
EL20 85.3 42.4
EL50 236.2 94.0

Executive summary:

6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithionewas tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 (version dated 13-Apr-2004). In order to in­vestigate the influence of the test item towards the daphnias, the swimming behaviour of the animals was recorded. The test was performed using five concentrations of “WAFs” (Water accommodated fractions) in the nominal range of 10 to 160 mg/L of6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione.Under the conditions used for the test, there was an immobilisa­tion of the daphnia at concentrations≥ 10 mg 6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione/L. As a conclusion of the analytical part of this study, it can be stated that the concentrations6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithioneremained sufficiently stable during incubation of 48hr.As a result of the supporting analyse it can be stated that the results of the biological part should be based on thenominal concentrationsapplied because the recoveries of the nominal concentrations were all >80%.The following EL-values (“Effective Loading”) were obtained:

	Basis: Nominal Concentrations [mg6-(dibutylamino)-1,3,5-triazine-2,4(1H,3H)-dithione/L](“WAF”)
	t24hr	t48hr
NOEL	80.0	80.0
LOEL	160.0	160.0
EL10	50.1	27.9
EL20	85.3	42.4
EL50[JM2]	236.2	94.0

 

 

The test is considered valid as all conditions for validity were met.