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EC number: 226-558-7 | CAS number: 5424-01-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 March 2013 to 20 March 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Version / remarks:
- 1996
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-aminopyrazine-2-carboxylic acid
- EC Number:
- 226-558-7
- EC Name:
- 3-aminopyrazine-2-carboxylic acid
- Cas Number:
- 5424-01-1
- Molecular formula:
- C5H5N3O2
- IUPAC Name:
- 3-aminopyrazine-2-carboxylic acid
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Molecular formula: C5H5N3O2
Molecular weight: 139.11
CAS Number: 424-01-1
Description: Tan powder
Batch: 203-2
Purity/Composition: 100 area%
Test substance storage: In refrigerator (2-8°C) protected from light, desiccated
Stability under storage conditions: Stable
Expiry date: 13 March 2014 (Retest date)
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Hygroscopic: Yes, store desiccated
Volatile: No
Reactivity: Reactive to light, moisture and oxygen
Test substance handling: No specific handling required for moisture and oxygen. Use amber-coloured glassware or wrap container in aluminium-foil
Stability in water: Unknown
Solubility in water: 2.4 g/l
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Singular samples for possible analysis were taken from all test concentrations and the control according to the schedule below.
Frequency: at t=0 h and t=48 h
Volume: 2 ml from the approximate centre of the test vessels
Storage: Samples were stored in a freezer until analysis.
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Additionally, singular reserve samples of 2 ml were taken for possible analysis. If not used, these samples were stored in a freezer.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The batch of Amiloride Compound 5 tested was a tan powder with a purity of 100 area% and completely soluble in test medium at the concentrations tested.
Preparation of test solutions started with a concentration of 100 mg/l applying 20 minutes of magnetic stirring followed by a 15-minute treatment with ultrasonic waves to accelerate the dissolving of the test substance in the test medium. The pH was adjusted to 6.5 using 1M NaOH (Merck, Darmstadt, Germany). The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. The final test solutions were clear and colourless to clear and slightly yellow in the highest test concentration.
Note that the preparation was performed under dimmed light.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species:Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by acyclical parthenogenesis under specified breeding conditions.
Source: In-house laboratory culture with a known history.
Reason for selection: This system has been selected as an internationally accepted invertebrate species.
Validity of batch: Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
Characteristics: For the test selection of young daphnids with an age of < 24 hours, from parental daphnids of more than two weeks old.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- -
- Post exposure observation period:
- no
Test conditions
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- 19.2-20.6
- pH:
- 6.8-8.0
- Dissolved oxygen:
- 8.8-9.3
- Salinity:
- not measured
- Nominal and measured concentrations:
- Nominal concentrations: 0.1, 1.0, 10, and 100 mg/l.
The actual concentrations were at the level of nominal (110% of nominal) and remained stable during the test (99% of initial). Consequently, the effects parameters were reported in terms of analytical confirmed nominal concentrations. - Details on test conditions:
- Test duration: 48 hours
Test type: Static
Test vessels: 100 ml, all-glass
Medium: Adjusted ISO medium
Number of daphnids : 20 each for the control and highest (limit) test concentration, 10 each for the remaining concentrations (combined range-finding test).
Loading: 5 per vessel containing 80 ml of test solution
Light: The test was performed under dimmed light conditions.
Feeding: No feeding
Aeration: No aeration of the test solutions.
Introduction of daphnids : Within 20 minutes after preparation of the test solutions. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: -
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: -
- Details on results:
- - Behavioural abnormalities:
- Other biological observations: none
- Mortality of control: no
- Other adverse effects control: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: The 48h-EC50 was 0.53 mg/l with a 95% confidence interval between 0.49 and 0.62 mg/l. - Reported statistics and error estimates:
- No EC50 could be calculated because the test substance proved to be non-toxic (EC50 > maximum concentration tested).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Amiloride Compound 5 did not induce acute immobilisation of Daphnia magna at an analytical confirmed nominal concentration of 100 mg/l after 48 hours of exposure.
The 48h-EC50 was beyond the range tested, i.e. exceeded an analytical confirmed nominal concentration of 100 mg/l.
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