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EC number: 260-280-7 | CAS number: 56602-77-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 June 1991 - 20 September 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- other: Environmental and Health Affairs Department Notification: No.5
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Pharmaceutical Affairs Bureau Notification: No.615
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: 49 Basic Industries Bureau Notification: No.392
- Deviations:
- no
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- 5.0 g (bulk) of the test substance were weighed into a 500 mL graduated flask, and ion-exchanged water was added up to the mark on the flask to prepare a 1 %-concentration (w/v) solution. This solution was used as the stock solution for preparing testing water at the different administration concentration levels. 5 liters each of the testing liquids - 0, 75, 100, 133 (mg/L), were prepared by diluting the stock solution with dechlorinated water as described below.
37.5 mL of 1 %-concentration (w/v) stock solution were diluted to 5 liters (75 mg/L)
50 mL of 1 %-concentration (w/v) stock solution were diluted to 5 liters (100 mg/L)
66.5 mL of 1 %-concentration (w/v) stock solution were diluted to 5 liters (133 mg/L)
Control: 5 liters of dechlorinated city water - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Length at study initiation: 3.0 - 3.8 cm (average: 3.4 cm)
- Weight at study initiation: 0.23 - 0.47 g (average: 0.33 g)
ACCLIMATION
- Acclimation period: 7 days before the test. Fish were kept in a fish-keeping bath in the room up to the start of the acclimatization after completion of immersion bathing. - Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 24.2 - 25.1 °C
- pH:
- 7.1 - 9.9
- Dissolved oxygen:
- 4.6 - 7.9 mg/L
- Nominal and measured concentrations:
- Based on the results of a preliminary test for 48-hour LC50 value (100 mg/L or above), the following administration concentration levels were set: 0, 75, 100, 133 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel fill volume: 5 L
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1
EFFECT PARAMETERS MEASURED
Survival was assessed at 1, 3, 6, 24 and 48 hours
- Reference substance (positive control):
- yes
- Remarks:
- Mercuric chloride
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 133 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- 48-hour LC50 value: 133 mg/L or above
No abnormality was detected in the behaviour of the test fish actually exposed to the test material and the test results reflect the results of the preliminary test, the value of the rate suggests that the test material is practically non-toxic to the test fish. - Results with reference substance (positive control):
- In parallel with the LC50 measurement test, test for determination of resistance of the same acclimated lot of Orizias latipes against mercuric chloride was conducted for the purpose of judging the validity of the results of the LC50 measurement test.
The results were as follows:
24-hour death rate at Hg 0.5 ppm: 30 % (specified value: 20 % or lower)
24-hour survival rate at Hg 1.0 ppm: 0 % (specified value: 20 % or lower)
The 24-hour death rate of 30 % deviates to some extent from the judgment criteria specified for the test in SOP T-414 (Acute toxicity test on fish for the setting of the concentration of test substances) of the Laboratory. However, since no abnormality was detected in the behaviour of the test fish actually exposed to the test substance and the test results described reflect the results of the preliminary test, the value of the rate suggests that the test substance is practically non-toxic to the test fish. Therefore, it was considered that there was no need for conducting a test again, and the results were judged to be valid. The results of the test conducted for determination of resistance against mercuric chloride were within the range of the specified value. - Sublethal observations / clinical signs:
Results
Test material concentration (mg/L)
Exposure period (hours)
1
3
6
24
48
0
0/10
0/10
0/10
0/10
0/10
75
0/10
0/10
0/10
0/10
0/10
100
0/10
0/10
0/10
0/10
0/10
133
0/10
0/10
0/10
0/10
0/10
Dead fish/ Tested fish
- Validity criteria fulfilled:
- yes
- Conclusions:
- 48-hour LC50 value: 133 mg/L or above.
- Executive summary:
The acute toxicity of the test material to fish was investigated in a study which was conducted in accordance with
Environment and Health Affairs Department Notification: No.5
Pharmaceutical Affairs Bureau Notification: No.615
49 Basic Industries Bureau Notification: No.392
During the study groups of 10 Orizias Latipes were exposed to test material concentrations of 0, 75, 100 and 133 mg/L. Survival was assessed at 1, 3, 6, 24 and 48 hours.
Under the conditions of the study none of the fish died and no abnormality was detected in the behaviour of the test fish exposed to the test material. The 48 hour LC50 was concluded to be in excess of 133 mg/L, the highest concentration tested.
Reference
Description of key information
48 -hour LC50 ≥ 133 mg/L (Oryzias latipes), Environmental and Health Affairs Department Notification: No.5, Environmental and Health Affairs Department Notification: No.5, 49 Basic Industries Bureau Notification: No.392 (1991)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 133 mg/L
Additional information
The acute toxicity of the test material to fish was investigated in a study which was conducted in accordance with Environment and Health Affairs Department Notification: No.5
Pharmaceutical Affairs Bureau Notification: No.615
49 Basic Industries Bureau Notification: No.392
During the study groups of 10 Orizias Latipes were exposed to test material concentrations of 0, 75, 100 and 133 mg/L. Survival was assessed at 1, 3, 6, 24 and 48 hours.
Under the conditions of the study none of the fish died and no abnormality was detected in the behaviour of the test fish exposed to the test material. The 48 hour LC50 was concluded to be in excess of 133 mg/L, the highest concentration tested.
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