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EC number: 233-251-1 | CAS number: 10101-50-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Sediment toxicity
Administrative data
Link to relevant study record(s)
- Endpoint:
- sediment toxicity: long-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- See the read-across report attached in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 42 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 285.9 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: Dissolved
- Basis for effect:
- other: Survival
- Duration:
- 42 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 627.2 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: Dissolved
- Basis for effect:
- other: Survival
- Duration:
- 42 d
- Dose descriptor:
- EC10
- Effect conc.:
- 188.5 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: Dissolved
- Basis for effect:
- other: Survival
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 96.4 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: Dissolved
- Basis for effect:
- growth rate
- Endpoint:
- sediment toxicity: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08/02/08-21/03/08
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- See the read-across report attached in Section 13.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 218 (Sediment-Water Chironomid Toxicity Test Using Spiked Sediment)
- Principles of method if other than guideline:
- This study was conducted according to the protocol, ''42- day Chronic Toxicity of Manganese to the freshwater amphipod crustacean, Hyalella aztecaThis protocol was designed to comply with methods described in the following references:
Environmental Canada(1997). Biological Test Methods: Test for Growth and Survival in Sediment Using the Freshwater Amphipod Hyalella azteca. Report EPS 1/RM/33.
U.S. Environmental Protection Agency(2000). Methods for measuring the toxicity and bioaccumulation of sediment associated contaminants with freshwater invertebrates. EPA 600/R-99/064,Duluth,MN. - GLP compliance:
- yes (incl. QA statement)
- Remarks:
- U.S. EPA GLP standard (40 CFR 792)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: were determined at test initiation and then determined twice a week
- Sampling method: Analytical samples from each treatment were collected for total recoverable and dissolved Mn analysis at test initiation. For the remainder of the test, samples for dissolved Mn analysis were collected from alternating replicates within each treatment twice a week.
- Sample storage conditions before analysis: Samples were preserved with nitric acid ( trade metal grade) to pH < 2 prior to analysis. - Vehicle:
- yes
- Details on sediment and application:
- PREPARATION OF SPIKED SEDIMENT
Artificially- formulated sediment was used as the sediment type in the toxicity test. Components and characteristics of the formulated sediments are provided in Table 1. Preparation instructions for the formulated sediments are detailed in OECD Guideline 218 (OECD 2004). - Test organisms (species):
- Hyalella azteca
- Details on test organisms:
- TEST ORGANISM
- Common name: terrestrial amphipods
- Source: Aquatic Biosystems (Fort Collins, CO, USA)
- Age of parental stock (mean and range, SD): Arrived at the testing laboratory as 5 to 7 day old organisms
- Age of animals at beginning of exposure: 7-9 days old
- Feeding during test
The organism were held in-house for 2 days as per PERL standard operating procedure(SOP) # 5102, with appropriate feeding s and water nenwals to maintain health in the organisms prior to test initiation.
ACCLIMATION
- Acclimation period: 2 days
- Acclimation conditions (same as test or not):The organism were held in-house for 2 days as per PERL standard operating procedure (# 5102, with appropriate feeding and water renewals to maintain health in the organisms prior to test initiation.
- Health during acclimation (any mortality observed): Visual observation, the organism appeared healthy at test initiation. - Study type:
- laboratory study
- Test type:
- flow-through
- Water media type:
- freshwater
- Type of sediment:
- artificial sediment
- Duration:
- 42 d
- Exposure phase:
- total exposure duration
- Post exposure observation period:
- Not reported
- Hardness:
- 104±5 mg/L as CaCO3
- Test temperature:
- 23±2°c
- pH:
- 7.3-8.1
- Dissolved oxygen:
- Dissolved oxygen concentration was maintained above 2.5 mg/L.
- Salinity:
- Not applicable
- Ammonia:
- <1.0 mg/L
- Nominal and measured concentrations:
- Nominal Concentration - 250, 500, 1000, 2000, 4000, and 8000 µg Mn/L
Average Measured Dissolved Mn Concentration - <62.5, 136.0, 285.9, 627.2, 1402.6, 3351.1, and 6663.6 µg/L - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1L glass jars with a mesh covered drainage hole.
- Sediment volume: Containers was filled with 100-mL of sterilised sand. A piece of nitex screen was placed in each chamber above the surface of the sand.
- Overlying water volume: The overlying volume of water in each chamber was approximately 500-600 mL.
EXPOSURE REGIME
- No. of organisms per container (treatment):10
- No. of replicates per treatment group:12
- No. of replicates per control / vehicle control:12
- Feeding regime: Daily. Flow to each test chamber was diverted for 2-3 hours to allow food to settle and become available to the organisms. If excess food or bacterial growth begins to form on the sediment surface, feeding may be suspended for one or more days.
- Type and preparation of food: Each chamber was fed YTC (Yeast, Trout Chow, Cereal Leaves).
- Amount of food: Each chamber was fed 1.0 mL of an 1800 mg/L stock.
RENEWAL OF OVERLYING WATER
- Details on volume additions: A Mariotte bottle was used to continuously deliver an appropriate volume of Mn stock solution to the diluter. A flow-splitting chamber was used for each test concentration( treatment) to promote mixing of the test solution and to allocate equally the test solution among the twelve replicate test chambers.
- Flow-rate: The stock solution was delivered to the dilutor at a rate of 5.0 mL/mixture. The flow rate to each test chamber was approximately 10 mL/min providing approximately 24 volume exchanges per replicate per day.
OVERLYING WATER CHARACTERISTCS
- Type of water : The dilution and control water used for this study was laboratory-blended water ( well water blended with reverse osmosis-treated water with a targeted hardness of 80- 120 mg/L as CaCO3).
- Alkalinity:129±5 mg/L as CaCO3
- Conductivity: 339±22 µS/cm
- Total organic carbon:<0.50 mg/L (DOC)
- Unionized ammonia:<1.0 mg/L
- Residual chlorine:37.3 mg/L
CHARACTERIZATION OF ARTIFICIAL SEDIMENT
Components and Characteristics of the formulated sediment are provided in Table 1. Preparation instruction for the formulated sediment is as per OECD guideline 218 (OECD 2004).
OTHER TEST CONDITIONS
- Photoperiod: 16- hours and 8-hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observations of live and dead organisms was recorded daily from test initiation until termination. Dead organism was recorded and immediately removed. At test day 28 organisms from four of the replicates was used for survival and growth measurements. The surviving organisms from the remaining eight replicates were replaced into water only chambers (without sand) and returned to the flow- through exposure system. On day 35, offspring was counted and removed from each chamber. Endpoint measured include survival (Day 28, 35, and 42), growth (as dry weight on Day 28 and 42), and reproduction (number of young/ femaleproduced from day 28 to Day 42). For dry weight measurement, organisms from each chamber was placed into a pre-weighed aluminium pan and dried for at least 12 hours at 105°C to determine dry weight
VEHICLE CONTROL PERFORMED: Yes
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Six test treatments was tested using a 0.5 dilution scheme.
- Range finding study
- Test concentrations: 320 µg Mn/L
- Results used to determine the conditions for the definitive study: These concentration were selected based on findings from a previous chronic life-cycle H. azteca Mn test where concentration up to 320 µg Mn/L resulted in no effect on exposed organism. - Reference substance (positive control):
- no
- Duration:
- 42 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 285.9 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- other: Survival
- Duration:
- 42 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 627.2 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- other: Survival
- Duration:
- 42 d
- Dose descriptor:
- EC10
- Effect conc.:
- 188.5 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- other: Survival
- Remarks on result:
- other: 95% CL
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 96.4 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL
- Details on results:
- Not reported
- Results with reference substance (positive control):
- Not reported
- Reported statistics and error estimates:
- All statistical analyses were performed using the mean dissolved Mn concentrations. Differences in survival (at Days 28, 35, and 42), growth (at day 28 and 42), and reproduction (at day 35 and 42) were evaluated using a statistical computer package (comprehensive Environmental Toxicity Information System [CETIS], McKinleyville, CA, USA, and Toxicity Relationship Analysis Program [TRAP], Duluth, MN, USA). If the data met the assumptions of normality and homogeneity, the NOEC and LOEC were estimated using an analysis of variance to compare (p≤0.05) organism performance in the experimental treatments with that observed in the control. The effective concentration required to reduce the survival, growth, or reproduction by 10% relative to control performance (EC10 and EC20) were estimated using a piecewise linear regression analysis. The median-lethal concentration (LC50) was also estimated using a piecewise linear regression analysis. Exposure concentration were log-transformed before determination of EC10 and EC20 and LC50 values.
- Validity criteria fulfilled:
- yes
- Remarks:
- Control survival was greater than 80% at the end of the test
- Conclusions:
- Under the conditions of the study the lowest NOEC (survival) was 0.29 mg Mn/L.
Referenceopen allclose all
Table 6. Survival Data1
Average Measured Dissolved Mn (mg/L) |
% Survival (±SD) |
||
Day 28 |
Dat 35 |
Day 42 |
|
<62.5 |
91±10 |
81±15 |
51±32 |
136.0 |
83±22 |
60±38 |
56±30 |
285.9 |
91±11 |
79±27 |
48±42 |
627.2 |
72±24* |
48±26* |
13±15* |
1402.6 |
58±35* |
37±34* |
20±23* |
3351.1 |
23±23* |
8±11* |
0±0* |
6663.6 |
0±0* |
- |
- |
1Survival is reported as the average survival within a treatment (combination of all replicates within a treatment).
* Significant less than control (p≤0.05).
SD = Standard Deviation
Table 7. Organism Growth Data (Biomass)1
Average Measured Dissolved Mn Concentration (µg/L) |
Average Dry weight per original organism in mg (±SD) |
|
Day 28 |
Day 42 |
|
<62.5 |
0.18±0.01 |
0.08±0.07 |
136.0 |
0.19±0.01 |
0.15±0.09 |
285.9 |
0.12±0.04 |
0.12±0.08 |
627.2 |
0.10±0.05* |
0.04±0.04 |
1402.6 |
0.16±0.052,* |
0.07±0.03 |
3351.1 |
0.03±0.01* |
- |
6663.6 |
- |
- |
1Biomass (average dry weight per original organism) is reported as a combination of all replicates within a treatment
2Day 28 average includes ‘outliers’ replicate.
*Significantly less than control (p≤0.05).
SD = Standard Deviation.
Table 8. Organism Growth Data1
Average Measured Dissolved Mn Concentration (µg/L) |
Average Dry weight per original organism in mg (±SD) |
|
Day 28 |
Day 42 |
|
<62.5 |
0.22±0.03 |
0.15±0.05 |
136.0 |
0.20±0.01 |
0.21±0.08 |
285.9 |
0.14±0.05* |
0.18±0.03 |
627.2 |
** |
** |
1402.6 |
** |
** |
3351.1 |
** |
** |
6663.6 |
** |
** |
1Dry weight is reported as the average weight per organism (combination of all replicates within a treatment).
*Significantly less than control (p≤0.05).
** Data was not used in statistical analysis (hypothesis testing) because a survival effect was observed at this concentration.
SD = Standard Deviation.
Table 8. Organism Reproduction Data1
Average Measured Dissolved Mn Concentration (µg/L) |
Average Reproduction (±SD) (young/female) |
|
Day 35 |
Day 42 |
|
<62.5 |
0.75±1.1 |
1.55±2.57 |
136.0 |
0.12±0.24* |
0.12±0.24 |
285.9 |
0.0±0.0* |
0.28±0.45 |
627.2 |
** |
** |
1402.6 |
** |
** |
3351.1 |
** |
** |
6663.6 |
** |
** |
1 Reproduction is reported as the average number of young per female (combination of all replicates within a treatment)
*Significantly less than control (p≤0.05).
** Data was not used in statistical analysis (hypothesis testing) because a survival effect was observed at this concentration.
SD = Standard Deviation
Table 10. Summary of Survival Statistics (µg Mn/L)1
Endpoint |
Survival |
||
28 day |
35 day |
42 day |
|
NOEC |
285.9 |
285.9 |
285.9 |
LOEC |
627.2 |
627.2 |
627.2 |
EC10 (95% Cl) |
568.6 (349.9-923.9) |
382.8(104.7-1399.5) |
188.47(27.47-1293.2) |
EC20 (95% Cl) |
752.6(494.0-1146.4) |
512.6(170.1-1544.3) |
255.9(51.9-1260.5) |
1NOEC = No Observed Effect Concentration. LOEC = Lowest Observed Effect Concentration. EC10= Effective Concentration for 10% reduction in survival relative to control performance. EC20 = Effective Concentration for 20% reduction in survival relative to control performance. CI – Confidence interval.
Table 11. Summary of Growth Statistically (µg Mn/L)1
Endpoint |
Mean Dry Biomass |
Dry Weight |
||
28 day2 |
42 day |
28 day |
42 day |
|
NOEC |
285.9 |
1402.6 |
136.0 |
285.9 |
LOEC |
627.2 |
>1402.6 |
285.9 |
>285.9 |
EC10( 95% CI) all replicates |
1349.8 (138.2-13184.3) |
N/A |
1720.5 9N/A) |
N/A |
EC10( 95% CI) Without outlier replicate |
1063.1 (549.0-2058.5) |
N/A |
96.4(12.9-720.7) |
N/A |
EC20(95% CI) all replicates |
1610.2(256.0-10126.5) |
N/A |
1949.0(N/A) |
N/A |
EC20(95% CI) Without outlier replicate |
1248.6(706.4-2206.9) |
N/A |
203.4(39.5-1046.9) |
N/A |
1NOEC = No Observed Effect Concentration. LOEC = Lowest Observed Effect Concentration. EC10= Effective Concentration for 10% reduction in survival relative to control performance. EC20 = Effective Concentration for 20% reduction in growth relative to control performance. CI– Confidence interval. N/A = Non-applicable/determinable.
Description of key information
Under the conditions of the study the lowest NOEC (survival) for the read across source substance was 0.29 mg Mn/L.
Key value for chemical safety assessment
- EC10, LC10 or NOEC for freshwater sediment:
- 0.2 mg/kg sediment dw
Additional information
Under the conditions of the study the lowest NOEC (survival) for the read across source substance was 0.29 mg Mn/L. The study was awarded a reliability score of 2 in accordance with the criteria set forth by Klimisch et al. (1997).
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