Octafluorocyclobutane

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1969

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

The limited information on the protocol does not allow verification of strict adherence to the guideline.

GLP compliance:

no

Test type:

fixed concentration procedure

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Octafluorocyclobutane

EC Number:

204-075-2

EC Name:

Octafluorocyclobutane

Cas Number:

115-25-3

Molecular formula:

C4F8

IUPAC Name:

octafluorocyclobutane

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source of test material: Organic Chemmicals Department of Jackson Laboratory.
- Expiration date and batch number: None given
- Purity test date: None given

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition & Stability of test material: not described in the report.
- Reactivity of the test substance with the solvent/vehicle /test medium: None

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Houseline air was used as a diluent to give the desired atmospheric concentration.
- Preliminary purification step: Unsaturates were removed from the sample Freon C-318 ® .

Test animals

Species:

rat

Strain:

other: ChR-CD

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Number : 6
- Source: not described in the report
- Age at study initiation: not described in the report
- Weight at study initiation: 258 to 268 grams
- Fasting period before study: not described in the report
- Housing: not described in the report
- Diet: not described in the report
- Water: not described in the report
- Acclimation period: not described in the report

ENVIRONMENTAL CONDITIONS
Not described in the report (no specific data on temperature, humidity or photoperiod).

Administration / exposure

Route of administration:

inhalation: gas

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: chamber (whole body)
- Exposure chamber volume: 16-liter exposure chamber
- Method of conditioning air: The test material was metered from an inverted cylinder into a heated copper coil.

TEST ATMOSPHERE
- Brief description of analytical method and equipment used: During the test, gas samples were taken every half hour from chamber exhaust port for atmosphere analysis. A triton X-100 column was used to separate the gases. Triton X-100 column is a Chromasorb ® W stainless steel column with 1/8'' diameter and 10' lenght, used in a Varian Aerograph Model 600-D gas chromatograph.
- Samples taken from breathing zone: yes

VEHICLE
- Composition of vehicle: Housline air used as a diluent
- Concentration of test material in vehicle: 50,000 ppm

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

5% (50000 ppm, v/v)

No. of animals per sex per dose:

6 male rats

Control animals:

no

Details on study design:

- Duration of observation period following administration: not detailed in study report
- Frequency of observations and weighing: not detailed in study report
- Necropsy of survivors performed: no
- Clinical signs including body weight: Irregular breathing; red ears, Normal weight gain

Results and discussion

Preliminary study:

None

Effect levels

Key result

Sex:

male

Dose descriptor:

LC50

Effect level:

> 50 000 ppm

Based on:

test mat.

Exp. duration:

4 h

Mortality:

Mortality ratio: 0/6

Clinical signs:

irregular respiration

Body weight:

Normal wright gain

Gross pathology:

None

Other findings:

Other observation: red ears

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

At the 5% level (50000 ppm, v/v), Freon C-318 (octafluorocyclobutane) purified by the procedure used for this sample was not toxic to rats during or after a four-hour exposure by inhalation.

Executive summary:

Acute toxicity of test material Freon® C-318 (octafluorocycobutane) was investigated in expermital test involving six male ChR-CD rats. Rats were exposed to a unique concentration of 50000 ppm of purified test material in a exposure chamber for four hours.

At the 5% level (50000 ppm, v/v), Freon C-318 (octafluorocyclobutane) purified by the procedure used for this sample was not toxic to rats during or after a four-hour exposure by inhalation.