sodium 5-(4-amino-6-(4-((E)-4-(4-(bis(2-hydroxyethyl)amino)-6-(8-hydroxy-7-((E)-phenyldiazenyl)-3,6-disulfonatonaphthalen-1-ylamino)-1,3,5-triazin-2-ylamino)-2-sulfonatostyryl)-3-sulfonatophenylamino)-1,3,5-triazin-2-ylamino)-4-hydroxy-3-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate

Toxicity to aquatic plants other than algae

Description of key information

ErC50 (7d) > 352 mg/L

Additional information

No information on the "toxicity to aquatic plants other than algae" of the Target Substance is available. However data on a Similar Substance has been taken into account for the assessment. More details on the substance similarity are reported in section 13.

The effects of test item on the growth of the monocotyledon species Lemna gibba was determined according to the principles of OECD 221 under GLP conditions. Each test concentration was freshly prepared with dilution water from a stock solution of 400 mg/L. The tested solutions were visually clear throughout the exposure. A semi-static exposure over 7 days was carried out. Based on a preliminary test, 5 nominal test item concentration levels were tested in a geometrical series with a dilution factor of 2.5: 10.2 - 25.6 -64 - 160 - 400 mg test item/L. Corrected for purity, the test concentrations were 8.98 - 22.5 - 56.3 - 141 - 352 mg/L. Three replicates were investigated for each test concentration and six for the control. Frond numbers were assessed on days 0, 3, 5 and 7. Environmental parameters (light, pH and temperature) were within the acceptable limits. The validity criteria of the test guideline were fulfilled. The concentrations of the test item and the control were analysed via spectrophotometric analysis at the beginning and end of the exposure and at every renewal day. Freshly prepared and old media were analysed. The measured concentrations of the test item in the fresh and old test media were in the range of 89 to 95 % and 92 to 103 % of the nominal values, respectively. All effect values are given below based on nominal test item concentration, corrected for purity.

Based on frond number (after 7 days):

 

NOEC (growth rate): 56.3 mg/L

LOEC (growth rate): 141 mg/L

ErC50: > 352 mg/L

ErC20: > 352 mg/L

ErC10: 52.4 mg/L (95% confidence limits 25.5 - 103)

NOEC (Yield): 56.3 mg/L

LOEC (Yield): 141 mg/L

EyC50: > 352 mg/L

EyC20: 65.4 mg/L (95% confidence limits < 8.98 – 140)

EyC10: < 8.98 mg/L

 

Based on dry weight (after 7 days):

 

NOEC (growth rate): < 8.98 mg/L

LOEC (growth rate): 8.98 mg/L

ErC50: > 352 mg/L

ErC20: 32.8 mg/L (21.8 - 45.8)

ErC10: < 8.98 mg/L

NOEC (Yield): < 8.98 mg/L

LOEC (Yield): 8.98 mg/L

EyC50: 58.1 (95% confidence limits 40.0 - 84.9)

EyC20: < 8.98 mg/L

EyC10: < 8.98 mg/L