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EC number: 248-890-1 | CAS number: 28188-41-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction
Administrative data
- Endpoint:
- one-generation reproductive toxicity
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data from secondary source
Data source
Reference
- Reference Type:
- secondary source
- Title:
- A 28 day feeding study in mice with isophthalonitrile.
- Author:
- HPVIS database
- Year:
- 2 006
- Bibliographic source:
- HPVIS, United States Environmental protection, 2006
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Reproductive Toxicity study of 1,3 Benzenedicarbonitrile was performed on rats by oral route
- GLP compliance:
- not specified
- Limit test:
- no
- Justification for study design:
- No data available
Test material
- Reference substance name:
- 1,3-Benzenedicarbonitrile
- Cas Number:
- 626-17-5
- Molecular formula:
- C8H4N2
- IUPAC Name:
- 1,3-Benzenedicarbonitrile
- Details on test material:
- - Name of test material (IUPAC name): 1,3-Benzenedicarbonitrile
- Common name: 1,3-Dicyanobenzene
- Molecular formula: C8H4N2
- Molecular weight: 128.134 g/mol
- Smiles notation: c1c(cccc1C#N)C#N
- InChl: 1S/C8H4N2/c9-5-7-2-1-3-8(4-7)6-10/h1-4H
- Substance type: Organic
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): 1,3-Benzenedicarbonitrile
- Common name: 1,3-Dicyanobenzene
- Molecular formula: C8H4N2
- Molecular weight: 128.134 g/mol
- Smiles notation: c1c(cccc1C#N)C#N
- InChl: 1S/C8H4N2/c9-5-7-2-1-3-8(4-7)6-10/h1-4H
- Substance type: Organic
Test animals
- Species:
- rat
- Strain:
- not specified
- Details on species / strain selection:
- No data available
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Details on test animals and env. conditions
TEST ANIMALS
- Source:
- Age at study initiation: 45 days
- Weight at study initiation: (P) Males: x-x g; Females: x-x g; (F1) Males: x-x g; Females: x-x g
- Fasting period before study:
- Housing:
- Use of restrainers for preventing ingestion (if dermal): yes/no
- Diet (e.g. ad libitum): fed PurinaB Certified Rodent Chow No. 5002 ad libitum.
- Water (e.g. ad libitum): fresh tap water ad libitum.
- Acclimation period:16 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C):
- Humidity (%):
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light):
IN-LIFE DATES: From: To:
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- not specified
- Details on exposure:
- Details on exposure
PREPARATION OF DOSING SOLUTIONS:
Test material mixed with fed
DIET PREPARATION
- Rate of preparation of diet (frequency): After initiation of mating on Study Day 81, diets were prepared weekly at constant concentration based on Week 11 body weight and food consumption data for the remainder of the study
- Mixing appropriate amounts with (Type of food )
- Storage temperature of food: No data available
VEHICLE
- Justification for use and choice of vehicle (if other than water):
- Concentration in vehicle:
- Amount of vehicle (if gavage): No data available
- Lot/batch no. (if required): No data available
- Purity: No data available - Details on mating procedure:
- - M/F ratio per cage:
- Length of cohabitation: 14days
- Proof of pregnancy: [vaginal plug / sperm in vaginal smear] referred to as [day 0 / day 1] of pregnancy
- After ... days of unsuccessful pairing replacement of first male by another male with proven fertility.
- Further matings after two unsuccessful attempts: [no / yes (explain)]
- After successful mating each pregnant female was caged (how):
- Any other deviations from standard protocol: - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 122 days
- Frequency of treatment:
- Daily
- Details on study schedule:
- No data available
Doses / concentrations
- Remarks:
- 0, 5, 10, 25 and 50 mg/kg/day.
- No. of animals per sex per dose:
- Total:250
0 mg/kg bw/day:25 male and 25 female
5 mg/kg bw/day:25 male and 25 female
10mg/kg bw/day:25 male and 25 female
25mg/kg bw/day:25 male and 25 female
50mg/kg bw/day:25 male and 25 female - Control animals:
- yes, plain diet
- Details on study design:
- No data available
- Positive control:
- No data available
Examinations
- Parental animals: Observations and examinations:
- Parental animals observation and examinations
CAGE SIDE OBSERVATIONS: yes
DETAILED CLINICAL OBSERVATIONS: Yes
Time schedule: daily
BODY WEIGHT: Yes
Time schedule for examinations: daily (Rats were mated for a total of 14 days during which no body weight measurements were recorded. )
FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study): Yes Food consumption was determined weekly.
Food consumption for each animal determined and mean daily diet consumption calculated as g food/kg body weight/day: Yes ( Rats were mated for a total of 14 days during which no food consumption measurements were recorded. )
Compound intake calculated as time-weighted averages from the consumption and body weight gain data: Yes / No / No data: No data available
WATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study): No data
Time schedule for examinations: - Oestrous cyclicity (parental animals):
- No data available
- Sperm parameters (parental animals):
- No data available
- Litter observations:
- No data available
- Postmortem examinations (parental animals):
- Postmortem examinations (Parent Animal)
SACRIFICE :
Male animals: All surviving animals [describe when, e.g. as soon as possible after the last litters in each generation were produced.]: yes
Maternal animals: yes ( Ten animals per sex from each group were killed after at least 31 days on the test diets, and the remaining 15 of each sex per group on day 122
GROSS NECROPSY: yes
HISTOPATHOLOGY / ORGAN WEIGHTS: yes
The tissues indicated in Table [#] were prepared for microscopic examination and weighed, respectively.:
macroscopic examination was performed - Postmortem examinations (offspring):
- No data available
- Statistics:
- No data available
- Reproductive indices:
- No data available
- Offspring viability indices:
- No data available
Results and discussion
Results: P0 (first parental generation)
General toxicity (P0)
- Clinical signs:
- not specified
- Dermal irritation (if dermal study):
- not specified
- Mortality:
- not specified
- Body weight and weight changes:
- no effects observed
- Description (incidence and severity):
- Compound-related effects on body weight and body weight gain with corresponding changes in food consumption were observed in males at 25 and 50 mg/kg/day and females at 10, 25 and 50 mg/kg/day during the 28- day feeding phase of the study.
- Food consumption and compound intake (if feeding study):
- not specified
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- not specified
- Haematological findings:
- not specified
- Clinical biochemistry findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Clinical chemistry analyses revealed significant increases in ALT in males at 10 and 50 mg/kg/day and in females at 10 mg/kg/day and higher. In addition, serum cholesterol was in 50mg/kg bw dose group males than controls.
- Urinalysis findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Urine volume was higher and specific gravity was lower in males at 25 and 50 mg/kg/day and females at 50 mg/kg/day.
- Behaviour (functional findings):
- not specified
- Immunological findings:
- not specified
- Organ weight findings including organ / body weight ratios:
- not specified
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- Histopathologic examinations revealed compound-related increases in the incidence of centrilobular hepatocytomegaly in males and females at 50 mg/kg/day and males at 25 mg/kg/day. Furthermore, a compound related increase in hyaline droplet formation was noted in the kidneys of males when compared to controls. No females exhibited this finding. Hyaline droplet formation in the proximal tubular epithelium of kidneys in male rats is indicative of abnormal accumulation of alpha-2 m-globulin, a protein reported to be unique to the rat
- Histopathological findings: neoplastic:
- not specified
- Other effects:
- not specified
Reproductive function / performance (P0)
- Reproductive function: oestrous cycle:
- not specified
- Reproductive function: sperm measures:
- not specified
- Reproductive performance:
- no effects observed
- Description (incidence and severity):
- parental reproductive parameters appeared to be unaffected . No compound-related effects were seen on mating, fertility or gestation length .Apparent compound-related reproductive effects on offspring survival in utero were noted. This was evidenced by an increase in the number of stillborn pups with a corresponding decrease in the number of live born pups and litter size at 50 mg/kg/day. Similar effects were not observed at lower dietary concentrations,(10,25 mg/kg /day)
Effect levels (P0)
open allclose all
- Dose descriptor:
- NOAEL
- Effect level:
- 25 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- female
- Basis for effect level:
- reproductive performance
- Remarks on result:
- other: No effects were seen on mating, fertility or gestation length
- Dose descriptor:
- LOEL
- Effect level:
- 50 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- female
- Basis for effect level:
- other: on the bases of an increase in the number of stillborn pups with a corresponding decrease in the number of live born pups and litter size at 50 mg/kg/day.
- Remarks on result:
- other: on the bases of an increase in the number of stillborn pups with a corresponding decrease in the number of live born pups and litter size at 50 mg/kg/day.
Target system / organ toxicity (P0)
- Critical effects observed:
- not specified
- System:
- other: not specified
- Organ:
- not specified
- Treatment related:
- not specified
- Dose response relationship:
- not specified
- Relevant for humans:
- not specified
Results: F1 generation
General toxicity (F1)
- Clinical signs:
- not specified
- Dermal irritation (if dermal study):
- not specified
- Mortality / viability:
- not specified
- Body weight and weight changes:
- not specified
- Food consumption and compound intake (if feeding study):
- not specified
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- not specified
- Haematological findings:
- not specified
- Clinical biochemistry findings:
- not specified
- Urinalysis findings:
- not specified
- Sexual maturation:
- not specified
- Organ weight findings including organ / body weight ratios:
- not specified
- Gross pathological findings:
- not specified
- Histopathological findings:
- not specified
- Other effects:
- not specified
Developmental neurotoxicity (F1)
- Behaviour (functional findings):
- not specified
Developmental immunotoxicity (F1)
- Developmental immunotoxicity:
- not specified
Effect levels (F1)
- Dose descriptor:
- other: not specified
- Based on:
- test mat.
- Sex:
- not specified
- Basis for effect level:
- other: not specified
- Remarks on result:
- other: not specified
Target system / organ toxicity (F1)
- Critical effects observed:
- not specified
- System:
- other: not specified
- Organ:
- not specified
- Treatment related:
- not specified
- Dose response relationship:
- not specified
- Relevant for humans:
- not specified
Overall reproductive toxicity
- Reproductive effects observed:
- no
- Treatment related:
- not specified
- Relation to other toxic effects:
- not specified
- Dose response relationship:
- not specified
- Relevant for humans:
- not specified
Applicant's summary and conclusion
- Conclusions:
- No Observed Adverse Effect Level (NOAEL) was considered to be 25mg/kg/day, on the bases of No compound-related effects were seen on mating, fertility or gestation length and LOAEL was considered to be 50mg/g bw , on the bases of an increase in the number of stillborn pups with a corresponding decrease in the number of live born pups and litter size at 50 mg/kg/day. When male and female rats were treated with 1,3-Benzenedicarbonitrile (626-17-5) orally.
- Executive summary:
The reproductive toxicity study of1,3-Benzenedicarbonitrile (626-17-5) was performed in male and female rats. The teat material mixed with fedPurinaB Certified Rodent Chow No. 5002 in dose concentration 0, 5, 10, 25 and 50 mg/kg/day. The concentration of test material administered to the rats was adjusted weekly in order to achieve as closely as possible the desired dietary intake of treatment. The achieved dosages of test material were calculated at the end of each week based on the dietary concentrations and body weights and food consumption for that week. After initiation of mating on Study Day 81, diets were prepared weekly at constant concentration based on Week 11 body weight and food consumption data for the remainder of the study. Control animals were fed PurinaB Certified Rodent Chow No. 5002 without test substance ad libitum and fresh tap water was available ad libitum. 25 rats of each sex were assigned to each group. Ten animals per sex from each group were killed after at least 31 days on the test diets, and the remaining 15 of each sex per group were exposed to test material for a total of at least 122 days. For the one-generation reproduction phase, mating commenced of Week 11. Rats were mated for a total of 14 days during which no body weight or food consumption measurements were recorded.
Compound-related effects on body weight and body weight gain with corresponding changes in food consumption were observed in males at 25 and 50 mg/kg/day and females at 10, 25 and 50 mg/kg/day during the 28- day feeding phase of the study. Histopathologic examinations revealed compound-related increases in the incidence of centrilobular hepatocytomegaly in males and females at 50 mg/kg/day and males at 25 mg/kg/day. Furthermore, a compound related increase in hyaline droplet formation was noted in the kidneys of males when compared to controls. No females exhibited this finding. Hyaline droplet formation in the proximal tubular epithelium of kidneys in male rats is indicative of abnormal accumulation of alpha-2 m-globulin, a protein reported to be unique to the rat. Clinical chemistry analyses revealed significant increases in ALT in males at 10 and 50 mg/kg/day and in females at 10 mg/kg/day and higher. In addition, serum cholesterol was in 50mg/kg bw dose group males than controls.
Urine volume was higher and specific gravity was lower in males at 25 and 50 mg/kg/day and females at 50 mg/kg/day. parental reproductive parameters appeared to be unaffected . No compound-related effects were seen on mating, fertility or gestation length .Apparent compound-related reproductive effects on offspring survival in utero were noted. This was evidenced by an increase in the number of stillborn pups with a corresponding decrease in the number of live born pups and litter size at 50 mg/kg/day. Similar effects were not observed at lower dietary concentrations,(10,25 mg/kg /day).Hence in the 28-day feeding phase ,LOEL was considered to be 5mg/kg bw in male rats because of the increased incidence of hyaline droplet formation in the kidneys of males at all dietary levels. The LOEL was considered to be 10mg/kg bw for females. Based on the reductions in body weight, body weight gain, food consumption and increases in ALT at 10 mg/kg/day and higher, While for the reproductive phase No Observed Adverse Effect Level (NOAEL) was considered to be 25mg/kg/day, on the bases ofNo compound-related effects were seen on mating, fertility or gestation length.and LOAEL was considered to be 50mg/g bw , on the bases of an increase in the number of stillborn pups with a corresponding decrease in the number of live born pups and litter size at 50 mg/kg/day.When male and femalerats were treated with1,3-Benzenedicarbonitrile (626-17-5)orally.
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