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EC number: 207-529-8 | CAS number: 479-27-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin irritation:
No skin irritating or corrosive property were reported for 1,8-naphthylenediamine in two 3D skin models.
Eye irritation:
No eye irritating or corrosive property is predicted for 1,8-naphthylenediamine in a 3D-cornea model.
Key value for chemical safety assessment
Skin irritation / corrosion
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin:
A study for predicting non-specific, corrosive potentials of 1,8 -naphthylenediamine was performed by using reconstructed human skin. The experiment was carried out on a reconstructed human epidermis EST-1000 (CellSystems, ST. Katharinen, Germany) for detection of topically applied skin corrosives with the test item. Corrosive skin effects of substances are defined as irreversible damage of skin; namely, visible necrosis through the epidermis and into the dermis, following the application of a test substance for up to 4 hours. A 100% concentration was tested on the skin/ epidermal equivalents in triplicate. For the determination of time related cytotoxic effects the incubation periods were 3 min. and 60 min. respectively. Thus the study was conducted in accordance with the 431 guideline as well as with an EC guideline (amending Council Directive 67/548/EEC, B.40 Skin Corrosion) using the test item concentrations and incubation periods recommended there. These tests are also related to the revised OECD 404 guideline "Acute Dermal Irritation/Corrosion". To check the reliability of this test procedure a blinded positive/negative control study is conducted at regular interval in the lab. The reliability check is always performed by two technicians, in two laboratories in parallel using the same batches of the control test items.
The test item was applied at a 100% concentration, i.e. 25 mg per insert (plus 50µl 0.9% NaCl to moisten and ensure good contact with the skin).
The test result shows that no corrosive property of the test item was determined by the assay used.
Another study which allows the hazard identification of irritant substances in accordance with UN GHS category 2, was performed. The experiment was carried out according to EU Test Method B.46 using commercially available reconstructed human epidermis (RHS) model EST-1000 (CellSystems, St.Katharinen, Germany). Undiluted test substance was applied topically to the RHS model, i.e. 30 mg per insert (plus 30 µg 0.9% NaCl to moisten and ensure good contact with the skin; three replicates). After an exposure period of 20 minutes, followed by a 42 hours post treatment incubation period, the cell viability was measured to be 99.56% in the MIT (Methylthiazoletetrazolium) conversion assay. The results of the concurrent negative control (NC, 0.9% NaCl) and positive control (PC, 5% SOS) demonstrated the viability (NC) and sensitivity (PC) of the test model. Thus, the results show that the test substance is considered to have no skin irritation category.
Eye:
In an vitro study for assessing ocular irritation of compounds a human epithelial corneal cell model is used. The model used is standardized and commercially available (SkinEthic™ Human Corneal Epithelial Model (HCE); SkinEthic, France).
Undiluted 1,8-naphthylenediamine was applied topically to the HCE tissue, i.e. 30 mg per insert (plus 30 µl PBS to moisten and ensure good contact with the skin; three replicates). After an exposure period of 60 minutes, followed by a 16 hours post-treatment incubation period, the cell viability was 57% (rounded) as measured by a MTT conversion assay. The results of the concurrent negative (NC, PBS) and positive control (PC, 1 H-1 ,2,4-Triazole-3-thiol) demonstrated the viability (NC) and sensitivity (PC) of the test model. The results show that 1,8 -naphthylenediamine is predicted as non-irritant under the conditions of this test method.
Justification for classification or non-classification
Due to the negative results of the 3D-skin models and the negative results of the 3D-cornea model a classification is not justified.
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