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EC number: 210-578-8 | CAS number: 619-08-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reveiwed journal
- Justification for type of information:
- Data is from peer reveiwed journal
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- Determination of toxicity of chemical on Vibrio qinghaiensis sp.nov.Q67 by inhibiting the bioluminescence of microorganisms.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - IUPAC Name: 2-chloro-4-nitrophenol
- Common Name: Nitrofungin
- Mol. formula: C6H4ClNO3
- Molecular Weight: 173.555 g/mol
- Smiles: c1(cc(c(O)cc1)Cl)[N+](=O)[O-]
- InChI: 1S/C6H4ClNO3/c7-5-3-4(8(10)11)1-2-6(5)9/h1-3,9H
- Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- - Method: The stock solutions of the compounds were prepared with distilled water and stored at 31C, and all the tested concentrations were nominal.
- Test organisms (species):
- other: Vibrio qinghaiensis nov.Q67
- Details on inoculum:
- - Laboratory culture: Department of Biology, East China Normal University.
- Initial biomass concentration: 5.87 × 10-3 - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 20 min
- pH:
- 8.8-9
- Nominal and measured concentrations:
- 1.018 mg/l Nominal concentration
- Details on test conditions:
- - No. of vessels per concentration (replicates): 9
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 20 min
- Dose descriptor:
- EC50
- Effect conc.:
- 292.891 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Inhibition of bioluminescence
- Remarks on result:
- other: Bioluminescence of Q67 was then determined by Veritas TM luminometer. (-log EC50 = 4.40 mol/l)
- Reported statistics and error estimates:
- Concentration–response curves (CRCs) of all phenolic compounds and their mixtures were plotted on origin 7.5 software.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the inhibition of Bioluminescence of Q67 due to the exposure of chemical 2-Chloro-4-nitrophenol for 20 min, the EC50 was 292.891 mg/l.
- Executive summary:
Determination of toxicity of chemical on Vibrio qinghaiensis sp.nov.Q67 by inhibiting the bioluminescence of microorganisms Vibrio qinghaiensis nov.Q67. Test was performed in 96 well microplate. In the stud 36 blank controls (use deionized water in place of the toxicant) for a toxicant were performed. Vibrio was very sensitive to the chemical and thus exposed for 20 min only. Based on the inhibition of Bioluminescence of Q67 due to the exposure of chemical 2-Chloro-4-nitrophenol for 20 min, the EC50 was 292.891 mg/l (-log EC50 = 4.40 mol/l).
Reference
Description of key information
Determination of toxicity of chemical on Vibrio qinghaiensis sp.nov.Q67 by inhibiting the bioluminescence of microorganisms Vibrio qinghaiensis nov.Q67. Test was performed in 96 well microplate. In the stud 36 blank controls (use deionized water in place of the toxicant) for a toxicant were performed. Vibrio was very sensitive to the chemical and thus exposed for 20 min only. Based on the inhibition of Bioluminescence of Q67 due to the exposure of chemical 2-Chloro-4-nitrophenol for 20 min, the EC50 was 292.891 mg/l (-log EC50 = 4.40 mol/l).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 292.891 mg/L
Additional information
Based on the various experimental data and prediction data for the target chemical study have been reviewed to determine the toxic nature of 2-chloro-4-nitrophenol (619-08-9) on the microorganisms. The studies are as mentioned below:
In the first key study for 2-chloro-4-nitrophenol (619-08-9), Ecotoxicology and Environmental Safety 74 (2011), Determination of toxicity of chemical on Vibrio qinghaiensis sp.nov.Q67 by inhibiting the bioluminescence of microorganisms Vibrio qinghaiensis nov.Q67. Test was performed in 96 well microplate. In the stud 36 blank controls (use deionized water in place of the toxicant) for a toxicant were performed. Vibrio was very sensitive to the chemical and thus exposed for 20 min only. Based on the inhibition of Bioluminescence of Q67 due to the exposure of chemical 2-Chloro-4-nitrophenol for 20 min, the EC50 was 292.891 mg/l (-log EC50 = 4.40 mol/l).
Similarly in the second supporting study for 2-chloro-4-nitrophenol (619-08-9), (International Journal Environment Research 2011, and SAR QSAR Environ Res. 20110), toxicity of 2-chloro-4-nitrophenol was checked on the microorganisms. Determination of toxicity of chemical 2-Chloro-4-nitrophenol on the growth of Tetrahymena Pyriformis. Test performed in the static system. After the exposure of chemical growth inhibition was measured where 50% inhibition observed. Based on the growth inhibition of Tetrahymena Pyriformis due to the exposure of chemical, the IGC50 was 0.275 mg/l (– Log IGC50 = 1.59 mm/l).
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