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EC number: 266-831-8 | CAS number: 67634-26-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 September 2016 - 28 November 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Secondary activated sludge (22-09-2016) was obtained from the wastewater treatment plant Nieuwgraaf in Duiven, The Netherlands. This plant is an activated sludge plant treating predominantly domestic wastewater. The activated sludge was preconditioned to reduce the endogenous respiration rates. To this end, 0.40 g Dry Weight (DW)/L of activated sludge was aerated for one week. The sludge was diluted in the bottles to 2.0 mg/L. The inoculum was not pre-exposed to the test substance.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 1 mg/L
- Based on:
- test mat.
- Remarks:
- coated on silica gel
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Test bottles:
The test was performed in 0.30 L BOD (biological oxygen demand) bottles with glass stoppers.
Nutrients, stocks and administration:
The nutrient medium of the Closed Bottle test contained per liter of deionized water; 8.5 mg KH2PO4, 21.75 mg K2HPO4, 33.4 mg Na2HPO4·2H2O, 22.5 mg MgSO4·7H2O, 27.5 mg CaCl2, 0.25 mg FeCl3·6H2O. Ammonium chloride was omitted from the medium to prevent nitrification. Accurate administering of the test substance was accomplished by preparing a solid stock of 3.0 mg of the test substance per g of silica gel in a 50-mL serum flask. Only part of the top layer of the silica gel was brought into contact with the test substance. The serum flask was closed with a screw top and the content was mixed vigorously. Subsequently 0.10 g of silica gel with the test substance was added to the test bottles. The resulting concentration of test substance in the bottles was 1.0 mg/L. Next the bottles were filled with nutrient medium with inoculum and closed. Sodium acetate was added to the bottles using a stock solution of 1.0 g/L.
Test procedure:
Use was made of 10 bottles containing only inoculum (inoculum blank), 10 bottles containing inoculum and silica gel (silica gel control), 10 bottles containing inocolum, silica gel and test substance, and 6 bottles with inoculum and sodium acetate (positive control). The concentrations of the test substance, and sodium acetate in the bottles were 1.0 and 6.7 mg/L, respectively. Each of the prepared solutions was dispensed into the respective group of BOD bottles so that all bottles were completely filled without air bubbles. The zero time bottles were immediately analyzed for dissolved oxygen using an oxygen electrode. The remaining bottles were closed and incubated in the dark. Two duplicate bottles of all series were withdrawn for analyses of the dissolved oxygen concentration at day 7, 14, 21, and 28. One extension from the protocol of the Closed Bottle test was introduced. The Closed Bottle test was prolonged by measuring the course of the oxygen decrease in the bottles of day 28 using a special funnel. This funnel fitted exactly in the BOD bottle. Subsequently, the oxygen electrode was inserted in the BOD bottle to measure the oxygen concentration. The medium dissipated by the electrode was collected in the funnel. After withdrawal of the oxygen electrode the medium collected flowed back into the BOD bottle, followed by removal of the funnel and closing of the BOD bottle.
Test conditions:
The pH of the media were 7.3 at the start of the test. The pH of the media at day 28 were also 7.3 (both test and controls). Temperatures were within the prescribed temperature range of 22 to 24°C. - Reference substance:
- acetic acid, sodium salt
- Remarks:
- purity > 99%
- Test performance:
- The test is valid as shown by an endogenous respiration <1.5 mg/L (i.e. 0.9 mg/L) at day 28. Furthermore, the differences of the replicate values at day 28 were less than 20%. Sodium acetate was degraded by 81% of its theoretical oxygen demand after 14 days. Oxygen concentrations were >0.5 mg/L in all bottles during the test period.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 28
- Sampling time:
- 28 d
- Details on results:
- - The substance is biodegraded by 28% at day 28 in the Closed Bottle test. In the prolonged Closed Bottle test the test substance was biodegraded by 48% at day 60. The test item therefore did not fulfill the criteria for readily biodegradation (>60%) after 28 days of incubation. Hence the substance should be classified as non readily biodegradable.
- The calculated theoretical oxygen demand (ThOD) of the substance is 2.5 g/g. The ThOD of sodium acetate is 0.8 g/g
- No toxicity control was used in this test since possible toxicity of the test substance to microorganisms degrading acetate is not considered relevant. Inhibition of the endogenous respiration of the inoculum by the test substance at day 7 was not detected. Therefore, no inhibition of the biodegradation due to the "high" initial test substance concentration is expected.
- Functioning of the test system was checked by testing the reference item (sodium acetate), which showed a normal biodegradation curve. - Results with reference substance:
- 81% after 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- Endogenous respiration was 0.9 mg/L at day 28, the differences of the replicate values at day 28 were < 20%, the reference item was degraded by 81% of its ThOD after 14 days, oxygen concentrations were >0.5 mg/L in all bottles throughout the test
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The substance is biodegraded by 28% at day 28 in the Closed Bottle test and should therefore be classified as not readily biodegradable under the test conditions in this study.
- Executive summary:
In order to assess the biodegradation of the test substance, a screening test was performed according to OECD TG 301D (Closed Bottle test) and under GLP conditions. In this study activated sludge (2 mg/L) from a domestic wastewater treatment plant was exposed to 1 mg/L of the substance for 28 days. The test substance was biodegraded by 28% at day 28 in the Closed Bottle test and should therefore be classified as not readily biodegradable. The acceptability criteria were met and therefore the test was considered to be valid.
Reference
Description of key information
In order to assess the biodegradation of the test substance, a screening test was performed according to OECD TG 301D (Closed Bottle test) and under GLP conditions. In this study activated sludge (2 mg/L) from a domestic wastewater treatment plant was exposed to 1 mg/L of the substance for 28 days. The test substance was biodegraded by 28% at day 28 in the Closed Bottle test and should therefore be classified as not readily biodegradable. The acceptability criteria were met and therefore the test was considered to be valid.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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