Reaction mass of 1-chlorodecane and 1-chlorotetraceane

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1995-12-01 to 1995-12-04

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC Guideline L133/118

Version / remarks:

EEC Guideline L133/118 from 1988-05-30

Deviations:

no

Principles of method if other than guideline:

The constant dilution factor for the 5 test substance concentration has not been fulfilled, but 5 different concentrations has been tested.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorotetradecane

EC Number:

219-368-0

EC Name:

1-chlorotetradecane

Cas Number:

2425-54-9

Molecular formula:

C14H29Cl

IUPAC Name:

1-chlorotetradecane

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Name and location of sewage treatment plant where inoculum was collected: Sewage sludge for a municipal STP
- Preparation of inoculum for exposure: The sewage sludge was treated by centrifugation and washing with drinking water (4x times).
- Initial biomass concentration: 3,5 g dry weight/L (1.4 g dry weight/L per test vessel)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

18-22 °C, test start 18-18.5 °C, during O2 measurement 19-20 °C

pH:

8.1

Dissolved oxygen:

24.32-24.68 mg O2/ L*h

Nominal and measured concentrations:

20 mg/L, 41.2 mg/L, 85.8 mg/L, 133 mg/L, 262.2 mg/L

Details on test conditions:

100 mL dest. water was added to the defined amount of test substance. 16 mL synthetic sewage water and 200 mL of the inoculum were added to the test substance and the volume was filled up to 500 mL with drinking water.
After 3 hours, the pH value has been determined and O2 consumption has been measured for 8 min.

2 controls without test substance; 3 reference controls with reference item; test vessels with 5 different test substance concentration

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 260 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other:

Remarks:

The test substance revealed no inhibition on the respiration of sewage sludge.

Details on results:

In the tested concentration range of 20 to 262.2 mg/L test substance, no inhibition on the respiration of sewage sludge by the test substance has been observed. Hence, a EC10 or an EC50 could not be determined.

Results with reference substance (positive control):

3,5-Dichlorophenol has been tested with a concentration of 5, 7, and 10 mg/L and an EC50 of 6.6 mg/L was measured.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the tested concentration range of 20; 41,2; 85,8; 133 and 262,2 mg / L, neither EC10 nor EC50 was achieved. The test substance did not have an inhibitory effect on the respiration of the activated sludge.