3-pyridyl dimethylcarbamate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001-26-04 to 2001-27-11

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 9888 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Static test (Zahn-Wellens method)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-pyridyl dimethylcarbamate

EC Number:

257-297-7

EC Name:

3-pyridyl dimethylcarbamate

Cas Number:

51581-32-9

Molecular formula:

C8H10N2O2

IUPAC Name:

pyridin-3-yl N,N-dimethylcarbamate

Specific details on test material used for the study:

colourless liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge:
1. Return activated sludge of biological waste water pretreatment plant DSM (BAV) and
2. Return activated sludge of municipal waste water treatment plant Welser Heide
- Preparation of inoculum for exposure: both return activated sludges were mixed, equal shares
- Water filtered: no

Duration of test (contact time):

18 d

Initial test substance concentration

Initial conc.:

112 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

TOC removal

Details on study design:

TEST CONDITIONS
- Test temperature: 23 - 20.5°C
- Sludge concentration: 1 g/L
- Substrate dilution: 167 μL/L (theoretical correspondance: 112 mg TOC/L)

SAMPLING
- Sampling frequency: T = 0, 1, 3, 7, 11, 18, 21 days

CONTROL AND BLANK SYSTEM
- Abiotic control
- Other: Reference substance: easily degradable substrate: Na-Benzoate

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (TOC removal)

Value:

>= 10 - <= 11

Sampling time:

18 d

Remarks on result:

readily biodegradable based on QSAR/QSPR prediction

Details on results:

see Table 1 in 'Any information on results incl. tables'

Any other information on results incl. tables

Table 1: Test results

Date	Days	Blank value (only sludge, no probe)	Control (Na-benzoate)	Test 1	Test 2	Blank (Probe without sludge)	Elimination in test substance 1	Elimination in test substance 2	Elimination Average
		mg TOC/L					%	%	%
24.09.01	0	5	344	121	122	117	0	0	0
25.09.01	1	7	235	120	121	115	3	3	3
27.05.01	3	9	39	117	117	114	7	8	7
01.10.01	7	12	12	117	112	112	9	15	12
05.10.01	11	11	13	117	110	114	9	15	12
12.10.01	18	14	-	11	10	122	103	103	103
15.10.01	21	13		10	11	111	103	102	102

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Pyridobase is readily biodegradable.

Executive summary:

The ready biodegradability of the test item was investigated in accordance with ISO 9888. In 2 test sequences, the test substance was applied at an initial concentration of 121 and 122 mg TOC/L, respectively. Biodegradation was followed by measurement of TOC removal.

The degradation extent of the test item at the end of the 21 day testing period was 103 and 102 %, respectively.