Indole

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data is from peer reviewed journals

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VI

Author:

Smyth et.al

Year:

1962

Bibliographic source:

American Industrial Hygiene Association Journal, 1962

Reference 2

Reference Type:

publication

Title:

Fragrance raw materials monographs

Author:

D.L.J. Opdyke

Year:

1974

Bibliographic source:

Food and Cosmetics Toxicology Volume 12, Issues 7–8, December 1974, Page 915

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

A range finding acute inhalation study was carried out to estimate the longest inhalation period which caused no mortality in the dosed rats.

GLP compliance:

not specified

Test type:

concentration x time method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Indole

EC Number:

204-420-7

EC Name:

Indole

Cas Number:

120-72-9

Molecular formula:

C8H7N

IUPAC Name:

1H-indole

Test material form:

solid

Details on test material:

Name: Indole
IUPAC Name: 1H-Indole
Molecular Formula: C8H7N
Molecular Weight: 117.15 g/mol
SMILES: c1ccc2[nH]ccc2c1
Substance Type: Organic
Physical State: Solid

Test animals

Species:

rat

Strain:

other: albino

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: The vapor-air mixture was generated by passing 2.5 liters/minute of dried air at room temperature through a fritted glass disc immersed to a depth of at least one inch in approximately 50 ml of the test chemical contained in a gas-washing bottle.
- Exposure chamber volume: 2.5 lt
- Method of holding animals in test chamber:
- Source and rate of air (airflow): The vapor-air mixture was generated by passing 2.5 liters/minute of dried air at room temperature through a fritted glass disc immersed to a depth of at least one inch in approximately 50 ml of the test chemical contained in a gas-washing bottle.
- Method of conditioning air:
- System of generating particulates/aerosols:
- Method of particle size determination:
- Treatment of exhaust air:
- Temperature, humidity, pressure in air chamber:

TEST ATMOSPHERE
- Brief description of analytical method and equipment used:
- Samples taken from breathing zone: yes/no
- Time needed for equilibrium of exposure concentration before animal exposure

VEHICLE
- Composition of vehicle (if applicable):
- Concentration of test material in vehicle (if applicable):
- Justification of choice of vehicle:
- Lot/batch no. (if required):
- Purity:

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution:
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.):

CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration:

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

8 h

Remarks on duration:

Inhalations are continued for time periods in a logarithmic series with a ratio of two extending from one-fourth to eight hours

Concentrations:

Concentrated vapor inhalation

No. of animals per sex per dose:

groups of six male or female albino rats

Control animals:

not specified

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC0

Based on:

test mat.

Exp. duration:

8 h

Remarks on result:

other: not specified

Mortality:

8 hr was the maximum period of concentrated vapour inhalation that caused no deaths in rats

Clinical signs:

other: not specified

Any other information on results incl. tables

Range -finding toxicity data

CAS	Concentrated vapor inhalation by rats, Maximum time for Zero deaths
120-72-9	8 h

Applicant's summary and conclusion

Interpretation of results:

other:

Conclusions:

8 hr was the maximum period of concentrated vapour inhalation that caused no deaths in rats.

Executive summary:

A range finding acute inhalation study was carried out to estimate the longest inhalation period which caused no mortality in the dosed rats. Concentrated vapor inhalation consists of subjecting groups of six male or female albino rats to a flowing stream of vapor-ladened air. The vapor-air mixture was generated by passing 2.5 liters/minute of dried air at room temperature through a fritted glass disc immersed to a depth of at least one inch in approximately 50 ml of the test chemical contained in a gas-washing bottle. Inhalations were continued for time periods in a logarithmic series with a ratio of two extending from one-fourth to eight hours, until the inhalation period killing about half the number of rats within 14 days was defined. 8 hr was the maximum period of concentrated vapour inhalation that caused no deaths in rats.