trans-1-(1-oxohexadecyl)-4-[(1-oxohexadecyl)oxy]-L-proline

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

HPLC was used to estimate the adsorption coefficient of trans-1-(1-oxohexadecyl)-4-[(1-oxohexadecyl)oxyl]-L-proline in soil and in sewage sludge.
Analytical column: Zorbax CN 4.6 mm * 250 mm - 5 µm
(Guard column: Zorbax CN 4.6 mm * 12.5 mm - 5 µm)
Mobile phase: methanol/0.01M citrate buffer pH 6.0 (55%/45% v/v)
Wave lenght: 210 nm
Temperature: 30°C
Flow rate: 1 mL/min

Details on study design: HPLC method:

Measured HPLC retention data were obtained for 11 reference items (acetanilide, atrazine, monuron, 2,5-dichloroaniline, linuron, naphthalene, benzoic acid phenylester, 1,2,3 trichlorobenzene, fenthion, phenanthrene and DDT). Based on these data, a calibration graph of Log k' versus log Koc was established to correlate the measured HPLC retention data of the test item with its adsorption coefficients (Koc) for soil and sewage sludge .

The capcity factors k' were calculated according to the following equation:
k' = (tR-t0)/t0
where:
tR: HPLC retention time in minutes
t0: HPLC dead time in minutes (determined with formamide)

The test item was prepared at a concentration of 1000 mg/L in three replicates.The reference items have been injected before and after the reference items to confirm that retention times had not drifted.

Type:

log Koc

Value:

< 1.25

Remarks on result:

other: Estimation by HPLC for soil

Type:

log Koc

Value:

< 1.46

Remarks on result:

other: Estimation by HPLC for sewage sludge

Details on results (HPLC method):

The retention times obtained for the three replicates of the test item were below the lowest retention time obtained for the reference items.

Validity criteria fulfilled:

yes

Conclusions:

In the conditions of this HPLC test, the log Koc values of trans-1-(1-oxohexadecyl)-4-[(1-oxohexadecyl)oxyl]-L-proline for soil and sewage sluge were estimated to be < 1.25 and < 1.46 respectively.

Executive summary:

The adsorption coefficients (Koc) on soil and on sewage sludge of trans-1-(1-oxohexadecyl)-4-[(1-oxohexadecyl)oxyl]-L-proline were estimated by a HPLC in accordance with OECD guideline 121.

Measured HPLC retention data were obtained for 11 reference items (acetanilide, atrazine, monuron, 2,5 dichloroaniline, linuron, naphathalene, benzoic acid phenylester, 1,2,3 trichlorobenzene, fenthion, phenanthrene and DDT). Based on these data, a calibration graph was established to correlate the measured HPLC retention data of the test item with its adsorption coefficients (Koc) for soil and sewage sludge .

The retention times obtained for the three replicates of the test item were below the lowest retention time obtained for the reference items.

In the conditions of this HPLC test, the log Koc values of trans-1-(1-oxohexadecyl)-4-[(1-oxohexadecyl)oxyl]-L-proline for soil and sewage sluge were estimated to be < 1.25 and < 1.46 respectively.

Key value for chemical safety assessment

Koc at 20 °C:

28.84