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EC number: 426-840-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start of Experiment: October 23, 1996 - Completion of Experiments: November 01, 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted April 04, 1984
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- SAMPLING AND ANALYSIS:
After 30 minutes of aeration at 21.5 °C, the first sample was taken for oxygen measurement over a period of about 10 minutes. Thereafter, in about 10-minute intervals the rest of the samples was submitted to measurement of oxygen concentration. The starting oxygen concentration was > 6.5 mg O2/l for all flasks.
Sampling was started with the control flask 1. Thereafter, the measurements were continued with the reference compound (flasks 2 - 6), control flask 7 (additional control), and the test article samples (flasks 8 - 12). Finally, the oxygen consumption of control flask 13 was determined. - Vehicle:
- no
- Details on test solutions:
- Please refer to "Details on test conditions" and tables in "Any other information in MM"
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: The test was performed with aerobic activated sludge from a domestic waste water treatment plant (ARA Ergolz II, Fullinsdorf /Switzerland).
- Preparation of sludge:
The sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. The latter procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (g) to its dry weight (g) determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 4 g dry material per litre, were made up with Soerensen buffer (pH 7). To this mixture, 50 ml OECD recommended synthetic sewage feed per litre was added daily, starting three days prior to use, and the sludge was aerated until use. Before use, the pH of the activated sludge was adjusted to 7.8 with 0.5 mol/l H2S04.
Synthetic sewage feed: 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O and 2.8 g K2HPO4 filled up to a final volume of 1 l with bidistilled water. - Test type:
- static
- Water media type:
- other: bidistilled water
- Limit test:
- no
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
- Hardness:
- Not specified.
- Test temperature:
- The test was performed at 21.5 °c.
- pH:
- Before use, the pH of the activated sludge was adjusted to 7.8 with 0.5 mol/l H2S04.
- Dissolved oxygen:
- Not specified.
- Salinity:
- Not specified.
- Conductivity:
- Not specified.
- Nominal and measured concentrations:
- The study examined nominal test article concentrations in the range from 3.2 mg/I to 100 mg/I.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL glass flasks
- Aeration: The test was carried out in glass flasks (500 ml) aerated with an air-flow of about 0.2 I/minute instead of 0.5 I/minute to prevent possible foam development
- Sludge concentration (weight of dry solids per volume): please refer to "Details on inoculum".
TEST CONCENTRATIONS
- Test concentrations: from 3.2 to 100 mg/L
For details on filling the flasks, please refer to section "Any other information on materails and methods incl. tables" - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol used as a reference compound.
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- The respiration rate of aerobic waste water microorganisms was practically not inhibited (-1.8 % to 2.1 %), when exposed to the test article concentrations in the range from 3.2 to 100 mg/I. Based on this the EC50 and EC20 (30 minutes) can be defined as
>100 mg/I. - Results with reference substance (positive control):
- The respiration rate of the activated sludge was only slightly inhibited (3.3 % and 5.3 %) at the two lowest concentrations of 1.0 and 3.2 mg/I. At 10, 32 and 50 mg/I, the respiration rate was inhibited by 11.5, 66.7 and 73.4 %, respectively.
The EC50 (30 minutes) determined by the Logit model (Ref. 1) was calculated to be 23.7 mg/I with the 95 % confidence limits of 23.1 - 24.4 mg/I.
The dose response line was: Logit = -4.37 + 1.380 x log (dose). The 95 % confidence limits for the slope were 1.379 - 1.380. - Validity criteria fulfilled:
- yes
- Conclusions:
- When SCARLET RN 1165 was assessed for its toxicity to waste water bacteria, the following result was obtained:
EC50 (30 minutes): >100 mg/I
EC20 (30 minutes): >100 mg/I - Executive summary:
The inhibitory effect of SCARLET RN 1165 on aerobic waste water microorganisms of activated sludge was investigated in a respiration test.
This study examined nominal test article concentrations in the range from 3.2 mg/l to 100 mg/l.
The respiration rate of aerobic waste water microorganisms was practically not inhibited (-1.8 to 2.1 %) when exposed to the test article concentrations in the range from 3.2 to 100 mg/I. The EC50 and EC20 (30 minutes) were therefore determined to be >100 mg/l.
The EC50 (30 minutes) for the reference compound 3,5-dichlorophenol, which was tested under the same conditions as the test article, was found to be at 23.7 mg/l and therefore lies within the range of 5 - 30 mg/l recommended by the test guidelines.
Reference
Flasks were filled up to 500 ml final volume according to the scheme given below:
Flask |
Identification |
Synth. sewage feed (ml) |
Activ. sludge1 (ml) |
Stock solution |
Water
(ml) |
Final conc.
(mg/l) |
|
I* (ml) |
II** (ml) |
||||||
1 |
Control |
16 |
200 |
- |
- |
284 |
- |
2 |
3,5-dichlorophenol |
16 |
200 |
- |
50 |
234 |
50 |
3 |
3,5-dichlorophenol |
16 |
200 |
- |
32 |
252 |
32 |
4 |
3,5-dichlorophenol |
16 |
200 |
- |
10 |
274 |
10 |
5 |
3,5-dichlorophenol |
16 |
200 |
- |
-3.2 |
280.8 |
3.2 |
6 |
3,5-dichlorophenol |
16 |
200 |
- |
1.0 |
283 |
1.0 |
7 |
Control |
16 |
200 |
- |
- |
284 |
- |
8 |
SCARLET RN 1165 |
16 |
200 |
100 |
|
184 |
100 |
9 |
SCARLET RN 1165 |
16 |
200 |
50 |
|
234 |
50 |
10 |
SCARLET RN 1165 |
16 |
200 |
32 |
|
252 |
32 |
11 |
SCARLET RN 1165 |
16 |
200 |
10 |
|
274 |
10 |
12 |
SCARLET RN 1165 |
16 |
200 |
3.2 |
|
280.8 |
3.2 |
13 |
Control |
16 |
200 |
- |
- |
284 |
- |
Influence of SCARLET RN 1165 on oxygen consumption of activated sludge.
Flask No. |
Chemical |
Concentration of test article (mg/l) |
Oxygen consumption (mg O2/l min.) |
Inhibition (%) |
Starting pH |
1 |
Control |
|
0.391 |
|
7.9 |
13 |
Control |
|
0.420 |
|
8.1 |
Mean Deviation (%) |
0.406 |
|
|
||
7.4 |
|
|
|||
8 |
SCARLET RN 1165 |
100 |
0.407 |
-0.4 |
8.0 |
9 |
SCARLET RN 1165 |
50 |
0.397 |
2.1 |
8.1 |
10 |
SCARLET RN 1165 |
32 |
0.407 |
-0.4 |
8.1 |
11 |
SCARLET RN 1165 |
10 |
0.410 |
-1.1 |
8.1 |
12 |
SCARLET RN 1165 |
3.2 |
0.413 |
-1.8 |
8.1 |
Influence of 3,5-dichlorophenol on oxygen consumption of activated sludge.
Flask No. |
Chemical |
Concentration of test article (mg/l) |
Oxygen consumption (mg O2/l min.) |
Inhibition (%) |
Starting pH |
1 |
Control |
|
0.391 |
|
7.9 |
13 |
Control |
|
0.420 |
|
8.1 |
Mean Deviation (%) |
0.406 |
|
|
||
7.4 |
|
|
|||
8 |
3,5-dichlorophenol |
50 |
0.108 |
73.41 |
7.9 |
9 |
3,5-dichlorophenol |
32 |
0.135 |
66.71 |
7.9 |
10 |
3,5-dichlorophenol |
10 |
0.359 |
11.51 |
7.9 |
11 |
3,5-dichlorophenol |
3.2 |
0.384 |
5.31 |
8.0 |
12 |
3,5-dichlorophenol |
1 |
0.392 |
3.31 |
8.0 |
1 Used for Logit estimation
Description of key information
When SCARLET RN 1165 was assessed for its toxicity to waste water bacteria, the following result was obtained:
EC50 (30 minutes) >100 mg/I
EC20 (30 minutes) >100 mg/I
Key value for chemical safety assessment
Additional information
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