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EC number: 200-090-3 | CAS number: 51-34-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-01-28 to 2008-02-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- The read-across justification is included as attachmant to Iuclid section 13.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- For measurement of the respiration rate a well-mixed sample of each test medium was poured into a BOD-flask after three hours incubation time, and was not further aerated.
- Details on test solutions:
- Based on the results of a pre-test, the test item was soluble at a concentration of at least 5 g/L in tap water.A stock solution with a nominal concentration of 5 g/L was prepared by dissolving 1250.1 mg of the test item in 250 mL tap water by intense stirring for fifteen minutes. Adequate volumes of this stock solution were diluted with tap water to prepare the test media with the lower test item concentrations. The final nominal concentrations tested were 10, 32, 100, 320 and 1000 mg/L.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Sampling site: ARA Ergolz II, Füllingsdorf, Switzerland- Concentration of suspended solid: 3 g per liter- Method of cultivation: prior to use the sludge was fed daily with 50 mL synthetic wastewater per liter and kept at room temperature under continuous aeration.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Hardness:
- no data
- Test temperature:
- 20+-2°C
- pH:
- 8.1 to 8.4
- Dissolved oxygen:
- 8.2 to 9.4 mg O2/L
- Salinity:
- no data
- Nominal and measured concentrations:
- The final nominal concentrations tested were 10, 32, 100, 320 and 1000 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: >500 ml- Volume of the test solution: 500 ml
- Test temperature: 20+-2°C
- Dissolved oxygen analyzer: oxygen electrode (WTW TriOxmatic 300) oxygen meter (WTW Oxi 539)
- Aeration: yes
- Preparation of synthetic sewage (filled up to 1 liter deionized water)16 g peptone11 g meat extract3 g urea0.7 g NaCl0.4 g CaCl2*2H200.2 g MgSO4*7H202.8 g K2HPO4
-Preparation of activated sludge: The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
-Preparation of stock solution of test item: A stock solution with a nominal concentration of 5 g/L was prepared by dissolving 1250.1 mg of the test item in 250 ml tap water by intense stirring for fifteen minutes. In this way a clear solution was obtained. Adequate volumes of this stock solution were diluted with tap water to prepare test media with the lower test item concentrations. Then, 16 ml synthetic wastewater and 200 ml activated sludge inoculum were added.
- Preparation of stock solution of reference control: 0.5 g 3,5-dichlorophenol was dissolved in 10 ml 1M NaOH and diluted to about 30 mL with purified water. Excess NaOH was neutralized with 0.5 M H2SO4 to the point of incipient precipitation. Thereafter, the mixture was diluted to 1 liter with purified water. The final pH was determined and to be in the range of 7 to 8.
TEST CONCENTRATIONS
- Test concentrations: 10, 32, 100, 320, 1000 mg/L
- Test concentrations reference control: 5, 16, 50 mg/L
- Replicates: Duplicates - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 933 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL = 913 to 947 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 847 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: determined as the calculated 3-hour EC15; 95% CL = 805 to 874 mg/L
- Results with reference substance (positive control):
- EC50 = 20 mg/L (3 hours)
- Validity criteria fulfilled:
- yes
- Conclusions:
- It is considered that the respiration inhibition of (-)-scopolamine butylbromide is low because EC50 was > 1000 mg/L and the NOEC is 847 mg/L under this test conditions.
Reference
Influence of Hyoscine Butylbromide and 3,5-Dichlorophenol on the Oxygen Consumption of Activated Sludge
Nominal Concentration (mg/L) | Oxygen consumption rate (mg O2 L-1 min-1) | Inhibition (%) | |
Test item | 10 | 1.365 | 3.2 |
32 | 1.423 | -0.9 | |
100 | 1.409 | 0.1 | |
320 | 1.420 | -0.7 | |
1000 | 1.069 | 24.2 | |
Reference control | 5 | 1.517 | -7.6 |
16 | 0.836 | 40.7 | |
50 | 0.169 | 88.0 | |
Blank control | 0 | 1.373 | |
0 | 1.446 | ||
Mean | 1.410 | ||
Deviation | 5.3 |
Up to and including the concentration of 320 mg/L the test item had no significant inhibitory effect (=15%) on the respiration rate of activated sludge after the incubation period of 3 hours. At the highest test item concentration of 1000 mg/L the inhibitory effect increased only slightly to 24%.
Description of key information
The read-across substance Hyoscine butylbromide was assessed for its toxicological potential to activated sludge in an aerobic respiration inhibition test over 3 hours according to OECD 209. Under this test conditions an EC50 of > 1000.0 mg/L was determined. A 3-hours NOEC of 847 mg/L was determined.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 847 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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