Phenyl phosphorodichloridate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1997-05-16 to 1997-06-26

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Phenyl phosphorodichloridate

EC Number:

212-220-6

EC Name:

Phenyl phosphorodichloridate

Cas Number:

770-12-7

Molecular formula:

C6H5Cl2O2P

IUPAC Name:

phenyl dichlorophosphate

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Direct dispersion in water. An amount of test material (200 mg) was dispersed in reconstituted water and the volume adjusted to 2 litres to give the 100 mg/l test concentration from which dilutions were made to give the remainder of the test series of 1.0, 1.8, 3.2, 5.6, 10, 18,32 and 56 mg/i.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

No

Test conditions

Hardness:

270 mg/L as CaCO3

Test temperature:

No data

pH:

7.8 ± 0.2 adjusted if necessary with NaOH or HCl

Dissolved oxygen:

Do data

Salinity:

Not applicable

Conductivity:

< 5µS /cm

Nominal and measured concentrations:

range finding study: 0.1, 1, 10 and 100 mg/L (Nominal)
Definitive test: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L (Nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL containing 200 mL of test solution
- Aeration: No auxiliary aeration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1 in the range finding test and 2 in the definitive test
- No. of vessels per control (replicates): 1 in the range finding test and 2 in the definitive test

TEST MEDIUM / WATER PARAMETERS
Stock Solutions
a) CaCI2.2H2O: 11.76 g/l
b) MgSO4.7H2O: 4.93 g/l
c) NaHCO3: 2.59 g/l
d) KCI: 0.23 g/l

ii) Preparation
25 ml of each of solutions a-d were added to each litre (final volume) of deionised water (conductivity < 5 JlS cm-1) pH equal to 7.8 :t 0.2, adjusted (if necessary) with NaOH or HCI.
The reconstituted water was aerated until the dissolved oxygen concentration was approximately air-saturation value.
The reconstituted water has an approximate theoretical total hardness of 270 mg/l as CaCO3"

RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study:
0% mortality at 1 mg/L and 100% mortality at 10 and 100 mg/L after 48 exposure. 0% mortality at 10 mg/L after 24h exposure.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality of control: No mortality observed
- Other adverse effects control:
- Observations made on the test media throughout the study showed the control, 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/l test concentrations to be clear, colourless solutions.

Results with reference substance (positive control):

No applicable

Any other information on results incl. tables

Range finding

Concentration (mg/L)	Cumulative mortality (Initial population = 10)
	24 hours	48 hours
Control	0	0
0.10	0	0
1.0	0	0
10	0	10
100	10	10

 

Definitive study

Nominal concentration (mg/L)	Cumulative immobilized Daphnia (Initial population: 10 per replicate)
	24 hours				48 hours
	R1	R2	Total	%	R1	R2	Total	%
Control	0	0	0	0	0	0	0	0
1.0	0	0	0	0	0	0	0	0
1.8	0	0	0	0	0	0	0	0
3.2	0	0	0	0	0	0	0	0
5.6	0	0	0	0	2	3	5	25
10	0	0	0	0	8	9	17	85
18	0	0	0	0	10	9	19	95
32	0	0	0	0	10	10	20	100
56	7	5	12	60	10	10	20	100
100	10	10	20	100	10	10	20	100

R1: Repliacte 1 and R2: replicate 2

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The phenyl dichlorophospohate was considered to be toxic to aquatic invertebrate according to GHS UN criteria (EC50 48h = 7.4 mg/L) but not classified according to CLP criteria.

Executive summary:

The acute toxicty on aquatic invertebrates of phenyl dichlorophosphine was evaluated on Daphnia magna according to OECD 202 test guideline.

The 48h-EC50 was determinated to be 7.4 mg/L.