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Diss Factsheets
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EC number: 204-368-5 | CAS number: 120-07-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Test start: 05 Dec 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- only basic data given; legally binding classification (KBwS, WGK 2)
- Qualifier:
- according to guideline
- Guideline:
- other: Water quality; fresh water algal growth inhibition test with Scenedesmus subspicatus and Selenastrum capricornutum
- Version / remarks:
- ISO 8692:1989; German version EN 28692:1993
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL- Source and lot/batch No.of test material: Merck (Name in KBWS database: N,N-Di(2hydroxyethyl)anilin)- Expiration date of the lot/batch: no data- Purity test date: no data
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: addition of 625 mg/L test item to 500 mL test medium, stirred for 0.5 h
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM - Strain: Scenedesmus subspicatus CHODAT - Source (laboratory, culture collection): laboratory culture - Age of inoculum (at test initiation): - Method of cultivation: cultured in 100-mL Erlenmeyer flasks in temperature-controlled room with uniform lighting (fluorescent tubes), pre-culture started with 7-d old algae from stock culture, pre-culture medium according to guideline
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 23± °C
- pH:
- stock solution (1250 mg/L): 7.26
- Nominal and measured concentrations:
- nominal: tested range: 156.25 to 1000 mg/L
- Details on test conditions:
- TEST SYSTEM - Test vessel: algae test apparatus with quasi-continuous determination of optical density (Abimed) - Initial cells density: 0.02 exteinction units = 10000 cells/mL - Control end cells density: not reported - No. of vessels per concentration (replicates): not reported - No. of vessels per control (replicates): not reported GROWTH MEDIUM - Standard medium used: yes - Detailed composition if non-standard medium was used: TEST MEDIUM / WATER PARAMETERS - Source/preparation of dilution water: test medium according to guideline OTHER TEST CONDITIONS - Sterile test conditions: yes - Adjustment of pH: no - Photoperiod: continuous - Light intensity and quality: 17 W/m² (fluorescent lamps) EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : - Determination of cell concentrations: fluorimeter (400-500 nm; emission: 685 nm) TEST CONCENTRATIONS - Spacing factor for test concentrations: - Justification for using less concentrations than requested by guideline: - Range finding study - Test concentrations: - Results used to determine the conditions for the definitive study:
- Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 418 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 393 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 172 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 156 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Validity criteria fulfilled:
- yes
Reference
Description of key information
With high probability acutely not harmful to algae.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 313 mg/L
- EC10 or NOEC for freshwater algae:
- 246 mg/L
Additional information
For the assessment of toxicity of the substance to algae experimental and calculated data available. The trend analysis was performed with QSAR Toolbox v4.4. The 72 -h ErC10 was determined to be 246 mg/L and the 72 -h EC50 was estimated to be 313 mg/L (based on growth rate BASF SE, 2021).
In addition experimental data are available from the “Commission for the Evaluation of Substances Hazardous to Waters” (KBWS) data sheet no. 1385, which is part of the German Federal Water Act (Article 19g). Based on the collected data a substance is classified according to its water-hazardous properties (WGK 1 to 3). 2,2 -phenyliminodiethanol was classified as WGK 2, which indicates a hazard to waters. As the list with the classified substances was published in the Bundesanzeiger (Federal Ministry of Justice, 2005. Bundesanzeiger No. 142a),
the classification is legally binding for the industry.
The KBWS contains an abstract on the algae test according to DIN EN 28692 (= ISO 8692). The test species wasDesmodesmus subspicatus. Test item concentrations were not analytically verified, but can be assumed to be stable based on the analytical results from an acute toxicity study to daphnids (BASF AG, 1998). The 72 -h ErC50 was determined to be 418 mg/L based on growth rate (72 -h EbC50 = 393 mg/L). The EC10 was only determined for biomass (72 -h EbC10 = 172 mg/L; KBWS, 1995). As the endpoint biomass is generally somewhat lower than the growth rate and can therefore be considered as a conservative value. Since there is only an abstract of the algae test available, the information on the test metods and substance details is limited. Therefore, the QSAR Toolbox calculations were marked as key studies and their endpoints were used as key values for the toxcity assessment and farther aquatic PNECs calculation.
Based on the available calculated and experimental data, 2,2 -phenyliminodiethanol is assessed to be not acutely harmful to algae with high probability. Chronic effects are not expected as well.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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