Octahydro-4,7-methano-1H-inden-5-yl acetate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2 June 1992 to 5 June 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to ISO 8192 in compliance with GLP. The reliability was set on 2 as no blank was included and results of reference substance are not stated.

Justification for type of information:

The information is used for read across to Cyclacet Dihydro.

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Source: Laboratory Waste Water purification plant (according to OECD confirmatory test for surfactants). Concentration of inoculum was 6 g/L dry matter.

Test type:

other: respiration measurement (O2-decrease) within the testing period

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Nominal and measured concentrations:

100, 180, 320, 560, 1000 mg/l

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Activated sludge was mixed with an artificial, standardized wastewater. The diluted test material is added.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The decrease of respiration is measured.

Reference substance (positive control):

yes

Remarks:

3,5 Dichlorophenol (CAS 25167-81-1)

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

245 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Key result

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

53 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: read from concentration/effect curve

Details on results:

Test concentration [mg/L] versus (vs) Inhibition [%]
100 vs 26.2
180 vs 35.1
320 vs 57.4
560 vs 77.1
1000 vs 88.7

Results with reference substance (positive control):

Test data have been confirmed by the reference substance (raw data).

Reported statistics and error estimates:

The EC 50 value was determined graphically from the relation between concentration and inhibition effect (semilogarithmic scale)

purity of the test substance not stated, test period of results not stated, results of reference substance not stated, no blank included

Validity criteria fulfilled:

no

Remarks:

no blank included, results of reference substance not stated, test period of results not stated

Conclusions:

An ASRI EC50 value of 245 mg/L was determined in a study conducted according to an appropriate test protocol, and in compliance with GLP.

Executive summary:

An activated sludge respiration inhibition test with Cyclacet has been performed, according to ISO 8192, which is similar to OECD TG 209. The sludge concentration was 6 g/l. The nominal test concentrations were 100, 180, 320, 560 and 1000 mg/l. A dose related increase in inhibition was seen from 26 to 88 mg/l. The 30min-EC50 and 30min-EC10 were found to be 245 and 53 mg/l respectively.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The information is based on read across

Justification for type of information:

The information on Cyclacet Dihydro is derived from the analogue Cyclacet. The full read across is presented in the Endpoint summary. The accompaning file is also attached there.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

245 mg/L

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Key result

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

53 mg/L

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Validity criteria fulfilled:

yes

Description of key information

The acute, EC50, and chronic, EC10, for micro-organisms is 245 and 53 mg/l, based on read across information of Cyclacet tested in an OECD TG 209 study.

Key value for chemical safety assessment

EC50 for microorganisms:

245 mg/L

EC10 or NOEC for microorganisms:

53 mg/L