Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-130-8 | CAS number: 103-63-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 1987
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 1-bromo-2-phenylethane
- EC Number:
- 203-130-8
- EC Name:
- 1-bromo-2-phenylethane
- Cas Number:
- 103-63-9
- Molecular formula:
- C8H9Br
- IUPAC Name:
- (2-bromoethyl)benzene
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- dihydrogen oxide
- Test material form:
- liquid
- Details on test material:
- colourless liquid
Constituent 1
impurity 1
- Specific details on test material used for the study:
- Abbreviation of test material: BEB
purity 98.7%
Test animals
- Species:
- rat
- Strain:
- CD-1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River(UK) Limited
- Females (if applicable) nulliparous and non-pregnant: [yes/no]
- Age at study initiation: five week old
- Weight at study initiation: male: 100 - 160 g, female; 100-143g
- Fasting period before study: one day
- Housing:54x33x20cm
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum):ad libitum
- Acclimation period:at least 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18-25
- Humidity (%): 40-70
- Air changes (per hr): 15 complete air change without re-circulating
- Photoperiod (hrs dark / hrs light): 12 hrs dark/12hrs light
IN-LIFE DATES: From: 1 day To:15 day
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- maize oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: The trest material was prepared at appropriate concentration in maize oil to permit administration at a constant volume-dosage of the m10 ml/kg bodyweight.
- Purity: 98.7%
MAXIMUM DOSE VOLUME APPLIED: 10 mf/kg body weight
- Doses:
- 200, 800, 2000 mg/kg bodyweight
- No. of animals per sex per dose:
- five male and five female rats per dose
- Control animals:
- not specified
- Details on study design:
- Preliminary study was carried out using three groups of one male and one female rat given a single oral administration of BEB at dosages in the range 200 - 800 mg/kg bodyweight, at a volume-dosage of 10 ml/kg in maize oil.
Dosage Mortality
(mg/kg ) Males Females
200 0/1 0/1
400 0/1 0/1
800 0/1 0/1
On the basis of this result the main study was carried out using four groups of five male and five female rats given a single oral administration of BEB within the range 800 - 2000 mg/kg - Statistics:
- Prohibit analysis by the method of Finney(1952) was used to determine the acute median lethal dosage, 95% confidence interval and slope of the dose response curve of the test materila for both sexes. The calculations were performed by the GLIM statics program (Baker and Nelder, 1978) using a special macro program developed by Baker (Baker, 1980).
An adaption of the classification of Hodge and Sterner (1949) was used in assessing the toxicity rating of the test material and the acute toxicity is expressed descriptively according to the LD50 value, as follows:
Classification of acute toxicity
LD50 (mg/kg) Classification
< 5 extreme toxicity
5 - 50 high toxicity
50 - 500 moderate toxicity
500 - 2000 slightly toxicity
>2000 low toxicity
Results and discussion
- Preliminary study:
- Preliminary study was carried out using three groups of one male and one female rat given a single oral administration of BEB at dosages in the range 200 - 800 mg/kg bodyweight, at a volume-dosage of 10 ml/kg in maize oil.
Dosage Mortality
(mg/kg ) Males Females
200 0/1 0/1
400 0/1 0/1
800 0/1 0/1
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 642 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 247 - <= 2 036
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 340 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 122 - <= 1 557
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 476 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 271 - <= 1 680
- Mortality:
- Animal died at dosage of 1086 mg/kg or above. The deathe occurred on Days 2, 3, 4, 5 or 12.
- Clinical signs:
- other: Ante mortem signs comprised lethargy, decreased motor activiity, prone posture, blanching, ataxia, muscle spasticity, bradypnoea, hyperpnoea, gasping, piloerection, ungroomed appearannce, pigmented staining of the snout, pigmented orbital secretion, saliv
- Gross pathology:
- Necropsy of the decedents revealed body surface staining, altered stomach and small intestine contents and occasional or single cases of red fluid and clotted blood on the brain, dark thymic lymph nodes, liver or lungs, dark areas on thegladular muicosa of the stomach and green fluid in the urinary bladder.
Necropsy of the surviving animalswas unremarkable.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Under the conditions of this study the acute oral median lethal dosage (LD50) were:
Male LD50 1642 mg/kg
Female LD50 1340 mg/kg
combined LD50 1476 mg/kg
Accordingly, BEB was assigned to the class 'slightly oral toxiciy', and correpond to category 4 as GHS classification. - Executive summary:
Under the conditions of this study the acute oral median lethal dosage (LD50) were:
Male LD50 1642 mg/kg
Female LD50 1340 mg/kg
combined LD50 1476 mg/kg
Accordingly, 1 -bromo-2 -phenylethane was assigned to the class 'slightly oral toxiciy', and correpond to category 4 as GHS classification.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.