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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003-04-14 to 2003-04-18
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD and GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate
EC Number:
228-528-9
EC Name:
Dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate
Cas Number:
6289-46-9
Molecular formula:
C10H12O6
IUPAC Name:
1,4-dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate
Details on test material:
- Name of test material (as cited in study report): DIMETHYLSUCCINYLOSUCCINATE

Test animals

Species:
rabbit
Strain:
New Zealand White

Test system

Type of coverage:
semiocclusive
Preparation of test site:
shaved
Vehicle:
water
Controls:
no
Amount / concentration applied:
0.5 g of the test substance moistened with 1.0 mL deionised water
Duration of treatment / exposure:
4 hours, residual test substance was wiped off with wet Kleenex-tissues
Observation period:
animals were examined for erythema/eschar and oedema as well as for other local alterations approximately 1, 24, 48 and 72 hours after patch removal. No further examinations were performed thereafter.
The animals were examined once daily for other than local changes of the skin.
Number of animals:
3 rabbits

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 24, 48 and 72 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
mean
Time point:
other: 24, 48 and 72 h
Score:
0
Max. score:
0
Irritant / corrosive response data:
Mean of 24, 48 and 72h for Animal #: 11/12/13
Erythema / Eschar scores: 0.0/0.0/0.0
Oedema scores: 0.0/0.0/0.0
Other effects:
No general toxic effects of the test substance were observed.
All exposed skin sites were normal at each examination term.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
DIMETHYLSUCCINYLOSUCCINATE is neither irritant nor corrosive to skin.
Executive summary:

The aim of this study was to investigate possible irritation or corrosion by the test substance following a single application to the intact skin of rabbits.

Methods and investigations were performed in accordance with the OECD Guideline 404

0.5 g "DIMETHYLSUCCINYLOSUCCINATE", moistened with deionised water, was applied via a patch to a site of 2.5 cm x 2.5 cm of the intact skin of each of 3 rabbits and covered by a semi-occlusive dressing. The duration of the exposure was 4 hours.

Investigations included: Body weights: at the start and at the termination of the test. General signs of toxicity: once daily. Skin examinations: 1, 24, 48 and 72 h after patch removal (p.a.).

No general toxic effects of the test substance were observed. All exposed skin sites were normal at each examination term.

Based on this findings DIMETHYLSUCCINYLOSUCCINATE is neither irritant nor corrosive to skin.